pLV3-CMV-Tmed10-3×FLAG-TurboID

pLV3-CMV-Tmed10-3×FLAG-TurboID-CopGFP-Puro plasmid

PVT96519

pLV3-CMV-Tmed10-3×FLAG-TurboID-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Tmed10(mouse)-3×FLAG-TurboID-CopGFP-Puro
Alias: NM_026775.4;1110014C03Rik; p23; p24delta1; Tmp21;
Gene lenght: 657bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Tmed10-3×FLAG-TurboID-CopGFP-Puro plasmid Description

pLV3-CMV-Tmed10-3×FLAG-TurboID-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Tmed10-3×FLAG-TurboID-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc atgtctggtt tgtttggccc actctcccgg 2341 cctggccctc tcccgtccgc ctggctcttt ctgttgctgc tcggccccag ctcggtcctc 2401 ggcatctcct tccatctgcc cgtgaactct cgcaagtgtc tccgagagga gatccacaag 2461 gacctgctgg tgacgggcgc ctacgagatc accgaccagt ctgggggcgc tggcggcctg 2521 cgcacccacc tcaagatcac agattctgct ggccatattc tgtatgccaa agaggatgca 2581 accaagggga agtttgcctt taccacggaa gactatgaca tgtttgaagt gtgttttgag 2641 agcaagggaa cagggcggat acctgaccaa ctcgtgattc tagacatgaa gcatggagta 2701 gaggcgaaaa attatgaaga gattgcaaaa gtcgagaaac tcaaaccact ggaggtggag 2761 ttacgacggc tcgaggacct ttccgagtcc attgttaatg actttgccta catgaagaag 2821 cgggaagagg agatgaggga cactaatgag tccacgaaca cccgggtcct gtacttcagc 2881 atcttttcca tgttctgcct cattggacta gccacctggc aggtcttcta cctgcgtcgc 2941 ttcttcaagg ccaagaagtt gatagagGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3001 CATGACATCG ACTACAAGGA TGACGATGAC AAGGGATCCA AAGACAATAC TGTGCCTCTG 3061 AAGCTGATCG CTCTCCTGGC TAATGGCGAG TTCCATAGTG GCGAACAGCT GGGAGAAACC 3121 CTGGGCATGT CCAGGGCCGC TATCAACAAG CACATTCAGA CTCTGCGCGA CTGGGGCGTG 3181 GACGTGTTCA CCGTGCCCGG AAAGGGCTAC TCTCTGCCCG AGCCTATCCC GCTGCTGAAC 3241 GCTAAACAGA TTCTGGGACA GCTGGACGGC GGGAGCGTGG CAGTCCTGCC TGTGGTCGAC 3301 TCCACCAATC AGTACCTGCT GGATCGAATC GGCGAGCTGA AGAGTGGGGA TGCTTGCATT 3361 GCAGAATATC AGCAGGCAGG GAGAGGAAGC AGAGGGAGGA AATGGTTCTC TCCTTTTGGA 3421 GCTAACCTGT ACCTGAGTAT GTTTTGGCGC CTGAAGCGGG GACCAGCAGC AATCGGCCTG 3481 GGCCCGGTCA TCGGAATTGT CATGGCAGAA GCGCTGCGAA AGCTGGGAGC AGACAAGGTG 3541 CGAGTCAAAT GGCCCAATGA CCTGTATCTG CAGGATAGAA AGCTGGCAGG CATCCTGGTG 3601 GAGCTGGCCG GAATAACAGG CGATGCTGCA CAGATCGTCA TTGGCGCCGG GATTAACGTG 3661 GCTATGAGGC GCGTGGAGGA AAGCGTGGTC AATCAGGGCT GGATCACACT GCAGGAAGCA 3721 GGGATTAACC TGGACAGGAA TACTCTGGCC GCTACGCTGA TCCGAGAGCT GCGGGCAGCC 3781 CTGGAACTGT TCGAGCAGGA AGGCCTGGCT CCATATCTGC CACGGTGGGA GAAGCTGGAT 3841 AACTTCATCA ATAGACCCGT GAAGCTGATC ATTGGGGACA AAGAGATTTT CGGGATTAGC 3901 CGGGGGATTG ATAAACAGGG AGCCCTGCTG CTGGAACAGG ACGGAGTTAT CAAACCCTGG 3961 ATGGGCGGAG AAATCAGTCT GCGGTCTGCC GAAAAGTAGG CGGCCGCTCG AGTCTAGAGG 4021 GCCCcggccg cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc 4081 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag 4141 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 4201 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 4261 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg 4321 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg 4381 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct 4441 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac 4501 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa 4561 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc 4621 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg 4681 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg 4741 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt 4801 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta 4861 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta 4921 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga 4981 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca 5041 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg 5101 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag 5161 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa 5221 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag 5281 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg 5341 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc 5401 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 5461 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 5521 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 5581 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 5641 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 5701 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 5761 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 5821 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 5881 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 5941 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 6001 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 6061 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 6121 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6181 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6241 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6301 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6361 gttgggcact gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct 6421 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6481 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6541 Tcgccttcgc cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt 6601 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6661 gactggaagg gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt 6721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6901 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6961 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 7021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7081 atgtatctta tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct 7141 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 7201 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 7261 aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa 7321 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 7381 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 7441 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 7501 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 7561 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7621 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 7741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 7981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8101 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 8161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 8521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 8941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9421 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 9481 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 9541 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 9601 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 9661 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 9721 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 9781 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 9841 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 9901 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.