pLV3-CMV-RBM22-3×FLAG-CopGFP-P

pLV3-CMV-RBM22-3×FLAG-CopGFP-Puro plasmid

PVT90488

pLV3-CMV-RBM22-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-RBM22(human)-3×FLAG-CopGFP-Puro
Alias: NM_018047.2;Cwc2; fSAP47; ZC3H16;
Gene lenght: 1260bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-RBM22-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-RBM22-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-RBM22-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgacctctct gggttccaac 2341 acctacaaca ggcagaactg ggaggatgcg gacttcccca ttctgtgcca gacatgtctt 2401 ggagaaaacc catatatccg aatgaccaaa gaaaagtatg ggaaggaatg caaaatctgt 2461 gccaggccat tcacagtgtt tcgctggtgc cctggagtcc gcatgcgttt caagaagact 2521 gaagtgtgcc aaacctgcag taaattgaag aatgtctgtc agacctgcct cttagaccta 2581 gagtatggcc tgcccatcca ggttcgtgac gcaggattgt cttttaaaga tgacatgcca 2641 aagtcagatg tcaacaaaga gtactataca cagaatatgg agagagagat ttctaactct 2701 gatggaacac ggccagttgg catgctgggg aaagccacat ctaccagtga catgctgctc 2761 aaactggccc ggaccacacc ctactacaaa aggaatcgac cccacatttg ctccttctgg 2821 gtgaaaggag agtgtaagag aggagaggaa tgtccataca gacatgagaa gcctacagat 2881 ccagatgacc cccttgctga tcagaatatt aaagaccgtt attacggaat caatgatcct 2941 gtagctgaca agcttctaaa gcgggcttca acaatgcctc ggctggaccc accagaggat 3001 aaaactatca ccacactata tgttggtggt ctaggtgata ccattactga gacagattta 3061 agaaatcatt tctaccagtt cggagagatc cggacgatca ctgttgtgca gagacagcag 3121 tgtgctttca tccagtttgc cacacggcag gctgcagaag tggctgctga gaagtccttt 3181 aataagttga ttgtaaatgg ccgcagactg aatgtgaaat ggggaagatc ccaggcagcc 3241 agaggaaaag aaaaagagaa agatggaact acagactctg ggatcaaact agaacctgtt 3301 ccaggattgc caggagctct tcctcctcct cctgcagcag aagaagaagc ctctgccaac 3361 tacttcaact tgcccccaag tggtcctcca gctgtggtga acattgctct gccaccgccc 3421 cctggcattg ctccaccccc acccccaggt tttgggccac acatgttcca cccaatggga 3481 ccaccccctc ctttcatgcg ggctccagga ccaatccact atccttctca ggaccctcag 3541 aggatgggag ctcatgctgg aaaacacagc agccccggat ccGACTACAA AGACCATGAC 3601 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGta gcggccgcGa 3661 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 3721 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 3781 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 3841 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 3901 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 3961 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 4021 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 4081 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 4141 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 4201 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 4261 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 4321 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 4381 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 4441 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 4501 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 4561 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 4621 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 4681 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 4741 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 4801 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 4861 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 4921 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 4981 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 5041 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5101 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 5161 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 5221 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5281 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5341 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5401 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 5461 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 5521 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 5581 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 5641 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5701 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5761 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5821 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 5881 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5941 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 6001 aattccgtgg tgttgtcggg gaaATCATCG TCCTTTCCTt ggctgctcgc ctgtgttgcc 6061 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 6121 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctTcg ccttcgccct 6181 cagacgagtc ggatctccct ttggGccgcc tccccgcctg gtacctttaa gaccaatgac 6241 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 6301 aattcactcc caacgaaGat aagatctgct ttttgcttgt actgggtctc tctggttaga 6361 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 6421 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 6481 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 6541 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 6601 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 6661 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 6721 tgtctggctc tagctaTCCC GCCCCTAACT CCGCCCAtcc cgcccctaac tccgcccagt 6781 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 6841 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 6901 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 6961 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7021 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 7081 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 7141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 7321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 7501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 7561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 7621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 7681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 7741 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 7801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 7861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8101 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8161 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8221 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8281 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8341 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8401 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8461 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 8521 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 8581 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 8641 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 8701 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 8761 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8821 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8881 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 8941 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9061 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 9121 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 9181 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 9241 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 9301 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 9361 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 9421 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 9481 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 9541 tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.