pLV3-CMV-GTF2I-3×Myc-EF1a-CopG

pLV3-CMV-GTF2I-3×Myc-EF1a-CopGFP-Puro plasmid

PVT86686

pLV3-CMV-GTF2I-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GTF2I(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_032999.4;BAP135; BTKAP1; DIWS; GTFII-I
Gene lenght: 2994bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×Myc-EF1a-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GTF2I-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-GTF2I-3×Myc-EF1a-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The CMV strong promoter can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of a 3×Myc tag, which facilitates recognition and tracking of the Myc-tagged target protein by anti-Myc antibodies in experiments such as Western blotting, immunoprecipitation, or immunofluorescence, thereby studying the expression level and interactions of the target protein with other proteins. The plasmid carries a CopGFP green fluorescent protein driven by the EF1a promoter for detecting the expression and localization of the target gene. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-GTF2I-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTTAACTTT 1801 TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC 1861 AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTATCGA 1921 TACTAGTATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC 1981 GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA 2041 TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG 2101 TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG 2161 CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC 2221 CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ATTCTAGAGC 2281 TAGCGAATTC GCCACCATGG CCCAAGTTGC AATGTCCACC CTCCCCGTTG AAGATGAGGA 2341 GTCCTCGGAG AGCAGGATGG TGGTGACATT CCTCATGTCA GCTCTCGAGT CCATGTGTAA 2401 AGAACTGGCC AAGTCCAAAG CCGAAGTGGC CTGCATTGCA GTGTATGAAA CAGACGTGTT 2461 TGTCGTCGGA ACTGAAAGAG GACGTGCTTT TGTCAATACC AGAAAGGATT TTCAAAAAGA 2521 TTTTGTAAAA TATTGTGTTG AAGAAGAAGA AAAAGCTGCA GAGATGCATA AAATGAAATC 2581 TACAACCCAG GCAAATCGGA TGAGTGTAGA TGCTGTAGAA ATTGAAACAC TCAGAAAAAC 2641 AGTTGAGGAC TATTTCTGCT TTTGCTATGG GAAAGCTTTA GGCAAATCCA CAGTGGTACC 2701 TGTACCATAT GAGAAGATGC TGCGAGACCA GTCGGCTGTG GTAGTGCAGG GGCTTCCGGA 2761 AGGTGTTGCC TTTAAACACC CCGAGAACTA TGATCTTGCA ACCCTGAAAT GGATTTTGGA 2821 GAACAAAGCA GGGATTTCAT TCATCATTAA GAGACCTTTT TTAGAGCCAA AGAAGCATGT 2881 AGGTGGTCGT GTGATGGTAA CAGATGCTGA CAGGTCAATA CTATCTCCAG GTGGAAGTTG 2941 TGGCCCCATC AAAGTGAAAA CTGAACCCAC AGAAGATTCT GGCATTTCCC TGGAAATGGC 3001 AGCTGTGACA GTAAAGGAAG AATCAGAAGA TCCTGATTAT TATCAATATA ACATTCAAGC 3061 AGGCCCTTCT GAAACTGATG ATGTTGATGA AAAACAGCCC CTATCGAAGC CTTTGCAAGG 3121 AAGCCACCAT TCTTCAGAGG GCAATGAAGG CACAGAAATG GAAGTACCAG CAGAAGATTC 3181 TACTCAACAT GTCCCTTCAG AAACAAGTGA GGACCCTGAA GTTGAGGTGA CTATTGAAGA 3241 TGATGATTAT TCTCCACCGT CTAAGAGACC AAAGGCCAAT GAGCTACCGC AGCCACCAGT 3301 CCCGGAACCC GCCAATGCTG GGAAGCGGAA AGTGAGGGAG TTCAACTTCG AGAAATGGAA 3361 TGCTCGCATC ACTGATCTAC GTAAACAAGT TGAAGAATTG TTTGAAAGGA AATATGCTCA 3421 AGCCATAAAA GCCAAAGGTC CGGTGACGAT CCCGTACCCT CTTTTCCAGT CTCATGTTGA 3481 AGATCTTTAT GTAGAAGGAC TTCCTGAAGG AATTCCTTTT AGAAGGCCAT CTACTTACGG 3541 AATTCCTCGC CTGGAGAGGA TATTACTTGC AAAGGAAAGG ATTCGTTTTG TGATTAAGAA 3601 ACATGAGCTT CTGAATTCAA CACGTGAAGA TTTACAGCTT GATAAGCCAG CTTCAGGAGT 3661 AAAGGAAGAA TGGTATGCCA GAATCACTAA ATTAAGAAAG ATGGTGGATC AGCTTTTCTG 3721 CAAAAAATTT GCGGAAGCCT TGGGGAGCAC TGAAGCCAAG GCTGTACCGT ACCAAAAATT 3781 TGAGGCACAC CCGAATGATC TGTACGTGGA AGGACTGCCA GAAAACATTC CTTTCCGAAG 3841 TCCCTCATGG TATGGAATCC CAAGGCTGGA AAAAATCATT CAAGTGGGCA ATCGAATTAA 3901 ATTTGTTATT AAAAGACCAG AACTTCTGAC TCACAGTACC ACTGAAGTTA CTCAGCCAAG 3961 AACGAATACA CCAGTCAAAG AAGATTGGAA TGTCAGAATT ACCAAGCTAC GGAAGCAAGT 4021 GGAAGAGATT TTTAATTTGA AATTTGCTCA AGCTCTTGGA CTCACCGAGG CAGTAAAAGT 4081 ACCATATCCT GTGTTTGAAT CAAACCCGGA GTTCTTGTAT GTGGAAGGCT TGCCAGAGGG 4141 GATTCCCTTC CGAAGCCCTA CCTGGTTTGG AATTCCACGA CTTGAAAGGA TCGTCCGCGG 4201 GAGTAATAAA ATCAAGTTCG TTGTTAAAAA ACCTGAACTA GTTATTTCCT ACTTGCCTCC 4261 TGGGATGGCT AGTAAAATAA ACACTAAAGC TTTGCAGTCC CCCAAAAGAC CACGAAGTCC 4321 TGGGAGTAAT TCAAAGGTTC CTGAAATTGA GGTCACCGTG GAAGGCCCTA ATAACAACAA 4381 TCCTCAAACC TCAGCTGTTC GAACCCCGAC CCAGACTAAC GGTTCTAACG TTCCCTTCAA 4441 GCCACGAGGG AGAGAGTTTT CCTTTGAGGC CTGGAATGCC AAAATCACGG ACCTAAAACA 4501 GAAAGTTGAA AATCTCTTCA ATGAGAAATG TGGGGAAGCT CTTGGCCTTA AACAAGCTGT 4561 GAAGGTGCCG TTCGCGTTAT TTGAGTCTTT CCCGGAAGAC TTTTATGTGG AAGGCTTACC 4621 TGAGGGTGTG CCATTCCGAA GACCATCGAC TTTTGGCATT CCGAGGCTGG AGAAGATACT 4681 CAGAAACAAA GCCAAAATTA AGTTCATCAT TAAAAAGCCC GAAATGTTTG AGACGGCGAT 4741 TAAGGAGAGC ACCTCCTCTA AGAGCCCTCC CAGAAAAATA AATTCATCAC CCAATGTTAA 4801 TACTACTGCA TCAGGTGTTG AAGACCTTAA CATCATTCAG GTGACAATTC CAGATGATGA 4861 TAATGAAAGA CTCTCGAAAG TTGAAAAAGC TAGACAGCTA AGAGAACAAG TGAATGACCT 4921 CTTTAGTCGG AAATTTGGTG AAGCTATTGG TATGGGTTTT CCTGTGAAAG TTCCCTACAG 4981 GAAAATCACA ATTAACCCTG GCTGTGTGGT AGTTGATGGC ATGCCCCCGG GGGTGTCCTT 5041 CAAAGCCCCC AGCTACCTGG AAATCAGCTC CATGAGAAGG ATCTTAGACT CTGCCGAGTT 5101 TATCAAATTC ACGGTCATTA GACCATTTCC AGGACTTGTG ATTAATAACC AGCTGGTTGA 5161 TCAGAGTGAG TCAGAAGGCC CCGTGATACA AGAATCAGCT GAACCAAGCC AGTTGGAAGT 5221 TCCAGCCACA GAAGAAATAA AAGAGACTGA TGGAAGCTCT CAGATCAAGC AAGAACCAGA 5281 CCCCACGTGG GGATCCGAAC AAAAACTAAT ATCAGAGGAA GATTTGGAAC AGAAACTAAT 5341 CAGTGAAGAA GATTTAGAGC AAAAGTTAAT TTCCGAGGAG GACTTATAGC GGCCGCGAAG 5401 GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA 5461 GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA 5521 CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA 5581 TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA 5641 GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG 5701 CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC 5761 GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC 5821 TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC TTGCTCAACT 5881 CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT GACCGGCGCC 5941 TACGCTAGAC GCCACCATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG 6001 CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC 6061 CCCCAAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG 6121 CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG 6181 CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT 6241 CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG 6301 CCGCGTGATC GGCGACTTCA AGGTGGTGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT 6361 CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA 6421 CGTGCTGGTG GGCAGCTTCG CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTT 6481 CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG 6541 GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGCTGCAC AGCAACACCG AGCTGGGCAT 6601 CGTGGAGTAC CAGCACGCCT TCAAGACCCC CATCGCCTTC GCCAGATCCC GCGCTCAGTC 6661 GTCCAATTCT GCCGTGGACG GCACCGCCGG ACCCGGCTCC ACCGGATCTC GCGAGGGCAG 6721 AGGAAGTCTT CTAACATGCG GTGACGTGGA GGAGAATCCC GGCCCTATGA CCGAGTACAA 6781 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC 6841 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG 6901 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG 6961 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG 7021 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC 7081 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT 7141 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT 7201 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC 7261 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC 7321 CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAAATC AACCTCTGGA 7381 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 7441 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT 7501 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG 7561 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC 7621 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA 7681 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA 7741 TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC 7801 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT 7861 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTCCGCC TTCGCCCTCA 7921 GACGAGTCGG ATCTCCCTTT GGCCGCCTCC CCGCCTGGTA CCTTTAAGAC CAATGACTTA 7981 CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT 8041 TCACTCCCAA CGAAAATAAG ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA 8101 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 8161 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 8221 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT 8281 TATTATTCAG TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT 8341 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 8401 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 8461 CTGGCTCTAG CTATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 8521 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 8581 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GACTTTTGCA GAGACGGCCC AAATTCGTAA 8641 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA 8701 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA 8761 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA 8821 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG 8881 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 8941 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 9001 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 9061 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 9121 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 9181 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 9241 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 9301 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 9361 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 9421 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 9481 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 9541 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 9601 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 9661 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 9721 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 9781 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 9841 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 9901 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 9961 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 10021 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 10081 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 10141 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 10201 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 10261 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 10321 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 10381 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 10441 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 10501 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 10561 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 10621 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 10681 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 10741 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 10801 GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC 10861 TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG 10921 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA 10981 GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG 11041 AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC 11101 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT 11161 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG 11221 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.