pLV3-CMV-AARS1-3×HA-Neo plasmi

pLV3-CMV-AARS1-3×HA-Neo plasmid

PVT86694

pLV3-CMV-AARS1-3×HA-Neo plasmid Information

Plasmid name: pLV3-CMV-AARS1(human)-3×HA-Neo
Alias: NM_001605.3;AARS; CMT2N; DEE29; EIEE29; HDLS2; TTD8
Gene lenght: 2904bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pCDH-CMV-MCS-EF1-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-AARS1-3×HA-Neo plasmid Description

pLV3-CMV-AARS1-3×HA-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of a 3×HA tag, facilitating experiments such as protein immunoprecipitation and co-immunoprecipitation. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pLV3-CMV-AARS1-3×HA-Neo plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC CATGGACTCT ACTCTAACAG CAAGTGAAAT CCGGCAGCGA 2341 TTTATAGATT TCTTCAAGAG GAACGAGCAT ACGTATGTTC ACTCGTCTGC CACCATCCCA 2401 TTGGATGACC CCACTTTGCT CTTTGCCAAT GCAGGCATGA ACCAGTTTAA ACCCATTTTC 2461 CTGAACACAA TTGACCCATC TCACCCCATG GCAAAGCTGA GCAGAGCTGC CAATACCCAG 2521 AAGTGCATCC GGGCTGGGGG CAAACATAAT GACCTGGACG ATGTGGGCAA GGATGTCTAT 2581 CATCACACCT TCTTCGAGAT GCTGGGCTCT TGGTCTTTTG GAGATTACTT TAAGGAATTG 2641 GCATGTAAGA TGGCTCTGGA ACTCCTCACC CAAGAGTTTG GCATTCCCAT TGAAAGACTT 2701 TATGTTACTT ACTTTGGCGG GGATGAAGCA GCTGGCTTAG AAGCAGATCT GGAATGCAAA 2761 CAGATCTGGC AAAATTTGGG GCTGGATGAC ACCAAAATCC TCCCAGGCAA CATGAAGGAT 2821 AACTTCTGGG AGATGGGTGA CACGGGCCCC TGTGGTCCTT GCAGTGAGAT CCACTACGAC 2881 CGGATTGGTG GTCGGGACGC CGCACATCTT GTCAACCAGG ACGACCCTAA TGTGCTGGAG 2941 ATCTGGAACC TTGTGTTCAT CCAGTATAAC AGGGAAGCTG ATGGCATTCT GAAACCTCTT 3001 CCCAAGAAAA GCATTGACAC AGGGATGGGC CTGGAACGAC TGGTATCTGT GCTGCAGAAT 3061 AAGATGTCCA ACTATGACAC TGACCTTTTT GTCCCTTACT TTGAAGCCAT TCAGAAGGGC 3121 ACAGGTGCCC GACCATACAC TGGGAAAGTT GGTGCTGAGG ATGCCGATGG GATTGACATG 3181 GCCTACCGGG TGCTGGCTGA CCACGCTCGG ACCATCACTG TGGCACTGGC TGATGGTGGC 3241 CGGCCTGACA ACACAGGGCG TGGATATGTG TTGAGACGGA TTCTCCGCCG AGCTGTCCGA 3301 TACGCCCATG AAAAGCTCAA TGCCAGCAGG GGCTTCTTTG CTACGTTAGT GGATGTTGTC 3361 GTCCAGTCCC TGGGAGATGC ATTTCCTGAG CTGAAGAAGG ACCCAGACAT GGTGAAGGAC 3421 ATCATTAATG AAGAAGAGGT GCAGTTTCTC AAGACTCTCA GCAGAGGGCG TCGCATCCTG 3481 GACAGGAAAA TTCAGAGCCT GGGAGACAGC AAGACCATTC CCGGAGACAC TGCTTGGCTC 3541 CTCTATGACA CCTATGGGTT TCCAGTGGAT CTGACTGGAC TGATTGCTGA AGAGAAGGGC 3601 CTGGTGGTAG ACATGGATGG CTTTGAAGAG GAGAGGAAAC TGGCCCAGCT GAAATCACAG 3661 GGCAAGGGAG CTGGTGGGGA AGACCTCATT ATGCTGGACA TTTACGCTAT CGAAGAGCTC 3721 CGGGCACGGG GTCTGGAGGT CACAGATGAT TCCCCAAAGT ACAATTACCA TTTGGACTCC 3781 AGTGGTAGCT ATGTATTTGA GAACACAGTG GCTACGGTGA TGGCTCTGCG CAGGGAGAAG 3841 ATGTTCGTGG AAGAGGTGTC CACAGGCCAG GAGTGTGGAG TGGTGCTGGA CAAGACCTGT 3901 TTCTATGCTG AGCAAGGAGG CCAGATCTAT GACGAAGGCT ACCTGGTGAA GGTGGATGAC 3961 AGCAGTGAAG ATAAAACAGA GTTTACAGTG AAGAATGCTC AGGTCCGAGG AGGGTATGTG 4021 CTACACATTG GAACCATCTA CGGTGACCTG AAAGTGGGGG ATCAGGTCTG GCTGTTTATT 4081 GATGAGCCCC GACGAAGACC CATCATGAGC AACCACACAG CTACGCACAT TCTGAACTTC 4141 GCCCTGCGCT CAGTGCTTGG GGAAGCTGAC CAGAAAGGCT CATTGGTTGC TCCTGACCGC 4201 CTCAGATTTG ACTTTACTGC CAAGGGAGCC ATGTCCACCC AACAGATCAA GAAGGCTGAA 4261 GAGATTGCTA ATGAGATGAT TGAGGCAGCC AAGGCCGTCT ATACCCAGGA TTGCCCCCTG 4321 GCAGCAGCGA AAGCCATCCA GGGCCTACGG GCTGTGTTTG ATGAGACCTA TCCTGACCCT 4381 GTGCGAGTCG TCTCCATTGG GGTCCCGGTG TCCGAGTTGC TGGATGACCC CTCTGGGCCT 4441 GCTGGCTCCC TGACTTCTGT TGAGTTCTGT GGGGGAACGC ACCTGCGGAA CTCGAGTCAT 4501 GCAGGAGCTT TTGTGATCGT GACGGAAGAA GCCATTGCCA AGGGTATCCG GAGGATTGTG 4561 GCTGTCACAG GTGCCGAGGC CCAGAAGGCC CTCAGGAAAG CAGAGAGCTT GAAGAAATGT 4621 CTCTCTGTCA TGGAAGCCAA AGTGAAGGCT CAGACTGCTC CAAACAAGGA TGTGCAGAGG 4681 GAGATCGCTG ACCTTGGAGA GGCCCTGGCC ACTGCAGTCA TCCCCCAGTG GCAGAAGGAT 4741 GAATTGCGGG AGACTCTCAA ATCCCTAAAG AAGGTCATGG ATGACTTGGA CCGAGCCAGC 4801 AAAGCCGATG TCCAGAAACG AGTGTTAGAG AAGACGAAGC AGTTCATCGA CAGCAACCCC 4861 AACCAGCCTC TTGTCATCCT GGAGATGGAG AGCGGCGCCT CAGCCAAGGC CCTGAATGAA 4921 GCCTTGAAGC TCTTCAAGAT GCACTCCCCT CAGACTTCTG CCATGCTCTT CACGGTGGAC 4981 AATGAGGCTG GCAAGATCAC GTGCCTGTGT CAAGTTCCCC AGAATGCAGC CAATCGGGGC 5041 TTAAAAGCCA GCGAGTGGGT GCAGCAGGTG TCAGGCTTGA TGGACGGTAA AGGTGGTGGC 5101 AAGGATGTGT CTGCACAGGC CACAGGCAAG AACGTTGGCT GCCTGCAGGA GGCGCTGCAG 5161 CTGGCCACTT CCTTCGCCCA GCTGCGCCTC GGGGATGTAA AGAACCTCGA GTACCCATAC 5221 GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT 5281 GATGTCCCAG ACTACGCATA GGATCCGCGG CCGCGAAGGA TCTGCGATCG CTCCGGTGCC 5341 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC 5401 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC 5461 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG 5521 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC 5581 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC 5641 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA 5701 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC 5761 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT 5821 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CGCTAGGCCA CCATGGCTGA 5881 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA 5941 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG 6001 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA 6061 GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT 6121 TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT 6181 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT 6241 GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG 6301 AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA 6361 GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA 6421 TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT 6481 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT 6541 GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT 6601 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT 6661 CTTCTGAGTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 6721 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 6781 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 6841 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 6901 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 6961 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 7021 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT 7081 TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 7141 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 7201 TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC 7261 GCCTGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA 7321 AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAAATAAGAT CTGCTTTTTG 7381 CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG 7441 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC 7501 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA 7561 TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT 7621 GAATATCAGA GAGTGAGAGG AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 7681 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 7741 CCAAACTCAT CAATGTATCT TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC 7801 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 7861 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGA 7921 CTTTTGCAGA GACGGCCCAA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 7981 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 8041 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 8101 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 8161 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 8221 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 8281 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 8341 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 8401 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 8461 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 8521 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 8581 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 8641 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 8701 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 8761 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 8821 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 8881 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 8941 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 9001 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 9061 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 9121 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 9181 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 9241 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 9301 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 9361 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 9421 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 9481 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 9541 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 9601 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 9661 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 9721 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 9781 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 9841 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 9901 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 9961 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 10021 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 10081 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC 10141 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 10201 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 10261 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 10321 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 10381 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG 10441 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG 10501 TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC 10561 GACGGCCAGT GCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.