pLV2-CMV-TIGAR-FLAG-Puro plasm

pLV2-CMV-TIGAR-FLAG-Puro plasmid

PVT86673

pLV2-CMV-TIGAR-FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-TIGAR(human)-FLAG-Puro
Alias: NM_020375.3;C12orf5;FR2BP
Gene lenght: 810bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLVX-FLAG-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-TIGAR-FLAG-Puro plasmid Description

pLV2-CMV-TIGAR-FLAG-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid carries a CMV strong promoter that can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of a 3×FLAG tag, which facilitates detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV2-CMV-TIGAR-FLAG-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA 2221 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 2281 TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 2341 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 2401 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 2461 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 2521 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 2581 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 2641 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 2701 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 2761 TCCATAGAAG ACACCGACTC TACTAGAGGA TCGCTAGCGC TACCGGACTC AGATCTCGAG 2821 GCCACCATGG CCGAGGAGCT GGTCTTAGAG AGGTGTGATC TGGAGCTGGA GACCAATGGC 2881 CGAGACCACC ACACGGCCGA CCTGTGCCGG GAGAAGCTGG TGGTGCGACG GGGCCAGCCC 2941 TTCTGGCTGA CCCTGCACTT TGAGGGCCGC AACTACGAGG CCAGTGTAGA CAGTCTCACC 3001 TTCAGTGTCG TGACCGGCCC AGCCCCTAGC CAGGAGGCCG GGACCAAGGC CCGTTTTCCA 3061 CTAAGAGATG CTGTGGAGGA GGGTGACTGG ACAGCCACCG TGGTGGACCA GCAAGACTGC 3121 ACCCTCTCGC TGCAGCTCAC CACCCCGGCC AACGCCCCCA TCGGCCTGTA TCGCCTCAGC 3181 CTGGAGGCCT CCACTGGCTA CCAGGGATCC AGCTTTGTGC TGGGCCACTT CATTTTGCTC 3241 TTCAACGCCT GGTGCCCAGC GGATGCTGTG TACCTGGACT CGGAAGAGGA GCGGCAGGAG 3301 TATGTCCTCA CCCAGCAGGG CTTTATCTAC CAGGGCTCGG CCAAGTTCAT CAAGAACATA 3361 CCTTGGAATT TTGGGCAGTT TGAAGATGGG ATCCTAGACA TCTGCCTGAT CCTTCTAGAT 3421 GTCAACCCCA AGTTCCTGAA GAACGCCGGC CGTGACTGCT CCCGCCGCAG CAGCCCCGTC 3481 TACGTGGGCC GGGTGGTGAG TGGCATGGTC AACTGCAACG ATGACCAGGG TGTGCTGCTG 3541 GGACGCTGGG ACAACAACTA CGGGGACGGC GTCAGCCCCA TGTCCTGGAT CGGCAGCGTG 3601 GACATCCTGC GGCGCTGGAA GAACCACGGC TGCCAGCGCG TCAAGTATGG CCAGTGCTGG 3661 GTCTTCGCCG CCGTGGCCTG CACAGTGCTG AGGTGCCTGG GCATCCCTAC CCGCGTCGTG 3721 ACCAACTACA ACTCGGCCCA TGACCAGAAC AGCAACCTTC TCATCGAGTA CTTCCGCAAT 3781 GAGTTTGGGG AGATCCAGGG TGACAAGAGC GAGATGATCT GGAACTTCCA CTGCTGGGTG 3841 GAGTCGTGGA TGACCAGGCC GGACCTGCAG CCGGGGTACG AGGGCTGGCA GGCCCTGGAC 3901 CCAACGCCCC AGGAGAAGAG CGAAGGGACG TACTGCTGTG GCCCAGTTCC AGTTCGTGCC 3961 ATCAAGGAGG GCGACCTGAG CACCAAGTAC GATGCGCCCT TTGTCTTTGC GGAGGTCAAT 4021 GCCGACGTGG TAGACTGGAT CCAGCAGGAC GATGGGTCTG TGCACAAATC CATCAACCGT 4081 TCCCTGATCG TTGGGCTGAA GATCAGCACT AAGAGCGTGG GCCGAGACGA GCGGGAGGAT 4141 ATCACCCACA CCTACAAATA CCCAGAGGGG TCCTCAGAGG AGAGGGAGGC CTTCACAAGG 4201 GCGAACCACC TGAACAAACT GGCCGAGAAG GAGGAGACAG GGATGGCCAT GCGGATCCGT 4261 GTGGGCCAGA GCATGAACAT GGGCAGTGAC TTTGACGTCT TTGCCCACAT CACCAACAAC 4321 ACCGCTGAGG AGTACGTCTG CCGCCTCCTG CTCTGTGCCC GCACCGTCAG CTACAATGGG 4381 ATCTTGGGGC CCGAGTGTGG CACCAAGTAC CTGCTCAACC TCAACCTGGA GCCTTTCTCT 4441 GAGAAGAGCG TTCCTCTTTG CATCCTCTAT GAGAAATACC GTGACTGCCT TACGGAGTCC 4501 AACCTCATCA AGGTGCGGGC CCTCCTCGTG GAGCCAGTTA TCAACAGCTA CCTGCTGGCT 4561 GAGAGGGACC TCTACCTGGA GAATCCAGAA ATCAAGATCC GGATCCTTGG GGAGCCCAAG 4621 CAGAAACGCA AGCTGGTGGC TGAGGTGTCC CTGCAGAACC CGCTCCCTGT GGCCCTGGAA 4681 GGCTGCACCT TCACTGTGGA GGGGGCCGGC CTGACTGAGG AGCAGAAGAC GGTGGAGATC 4741 CCAGACCCCG TGGAGGCAGG GGAGGAAGTT AAGGTGAGAA TGGACCTGCT GCCGCTCCAC 4801 ATGGGCCTCC ACAAGCTGGT GGTGAACTTC GAGAGCGACA AGCTGAAGGC TGTGAAGGGC 4861 TTCCGGAATG TCATCATTGG CCCCGCCGAA TTCGATTACA AGGATGACGA CGATAAGTGA 4921 GGATCCCGCG ACTCTAGATA ATTCTACCGG GTAGGGGAGG CGCTTTTCCC AAGGCAGTCT 4981 GGAGCATGCG CTTTAGCAGC CCCGCTGGGC ACTTGGCGCT ACACAAGTGG CCTCTGGCCT 5041 CGCACACATT CCACATCCAC CGGTAGGCGC CAACCGGCTC CGTTCTTTGG TGGCCCCTTC 5101 GCGCCACCTT CTACTCCTCC CCTAGTCAGG AAGTTCCCCC CCGCCCCGCA GCTCGCGTCG 5161 TGCAGGACGT GACAAATGGA AGTAGCACGT CTCACTAGTC TCGTGCAGAT GGACAGCACC 5221 GCTGAGCAAT GGAAGCGGGT AGGCCTTTGG GGCAGCGGCC AATAGCAGCT TTGCTCCTTC 5281 GCTTTCTGGG CTCAGAGGCT GGGAAGGGGT GGGTCCGGGG GCGGGCTCAG GGGCGGGCTC 5341 AGGGGCGGGG CGGGCGCCCG AAGGTCCTCC GGAGGCCCGG CATTCTGCAC GCTTCAAAAG 5401 CGCACGTCTG CCGCGCTGTT CTCCTCTTCC TCATCTCCGG GCCTTTCGAC CTGCAGCCCA 5461 AGCTTACCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA 5521 GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG 5581 ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG 5641 GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA 5701 CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT 5761 TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC 5821 CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG 5881 GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG 5941 CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG 6001 TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG 6061 GTGCCTGACC GCGTCTGGAA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 6121 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 6181 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 6241 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 6301 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 6361 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 6421 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG 6481 ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 6541 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 6601 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 6661 GCCTCCCCGC CTGGAATTAA TTCTGCAGTC GAGACCTAGA AAAACATGGA GCAATCACAA 6721 GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG 6781 AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG 6841 TAGATCTTAG CCACTTTTTA AAAGAAAAGA GGGGACTGGA AGGGCTAATT CACTCCCAAC 6901 GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTAGC 6961 AGAACTACAC ACCAGGGCCA GGGGTCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC 7021 TAGTACCAGT TGAGCCAGAT AAGGTAGAAG AGGCCAATAA AGGAGAGAAC ACCAGCTTGT 7081 TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG AGAAGTGTTA GAGTGGAGGT 7141 TTGACAGCCG CCTAGCATTT CATCACGTGG CCCGAGAGCT GCATCCGGAG TACTTCAAGA 7201 ACTGCTGATA TCGAGCTTGC TACAAGGGAC TTTCCGCTGG GGACTTTCCA GGGAGGCGTG 7261 GCCTGGGCGG GACTGGGGAG TGGCGAGCCC TCAGATCCTG CATATAAGCA GCTGCTTTTT 7321 GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA 7381 GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC 7441 CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA 7501 ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT TGCAAAGAAA 7561 TGAATATCAG AGAGTGAGAG GCCTTGACAT TGCTAGCGTT TTACCGTCGA CCTCTAGCTA 7621 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 7681 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 7741 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 7801 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 7861 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 7921 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 7981 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 8041 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 8101 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 8161 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 8221 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 8281 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 8341 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 8401 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 8461 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 8521 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 8581 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 8641 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 8701 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 8761 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 8821 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 8881 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 8941 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 9001 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 9061 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 9121 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 9181 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 9241 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 9301 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 9361 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 9421 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 9481 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 9541 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 9601 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 9661 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 9721 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 9781 AGTGCCACCT GACGTCGACG GATCGGGAGA TCAACTTGTT TATTGCAGCT TATAATGGTT 9841 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 9901 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCAAC TGGATAACTC 9961 AAGCTAACCA AAATCATCCC AAACTTCCCA CCCCATACCC TATTACCACT GCCAATTACC 10021 TGTGGTTTCA TTTACTCTAA ACCTGTGATT CCTCTGAATT ATTTTCATTT TAAAGAAATT 10081 GTATTTGTTA AATATGTACT ACAAACTTAG TAGTTTTTAA AGAAATTGTA TTTGTTAAAT 10141 ATGTACTACA AACTTAGTAG T

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.