pT7-KLHL20-6×His plasmid

pT7-KLHL20-6×His plasmid

PVT86314

pT7-KLHL20-6×His plasmid Information

Plasmid name: pT7-KLHL20(human)-6×His
Alias: NM_014458.4;KHLHX; KLEIP; KLHLX
Gene lenght: 1827bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pET-28a
Forward primer: T7
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-KLHL20-6×His plasmid Description

pT7-KLHL20-6×His This is a prokaryotic inducible low-copy expression plasmid. The plasmid contains a T7-LacO promoter system that, under the induction of IPTG or lactose and its analogs, initiates the expression of the human KLHL20 gene. The N-terminus of the KLHL20 gene in the plasmid carries His, Thrombin, and T7 protein tags, while the C-terminus has a His tag, which facilitates subsequent studies on the detection, purification, and localization of the expressed protein.

pT7-KLHL20-6×His plasmid Sequence

1 TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG 61 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC 121 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG 181 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC 241 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 301 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC 361 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 421 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT 481 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 541 TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT 601 TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA 661 ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC 721 GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA 781 AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC 841 AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC 901 CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC 961 AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT 1021 TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG 1081 TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA 1141 TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC 1201 CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG 1261 TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA 1321 TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC 1381 CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA 1441 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 1501 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 1561 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 1621 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 1681 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 1741 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 1801 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 1861 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 1921 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 1981 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 2041 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 2101 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 2161 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC 2221 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG 2281 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA 2341 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG 2401 GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT 2461 GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG 2521 GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC 2581 GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG 2641 AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT 2701 GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA 2761 ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG 2821 TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG 2881 TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC 2941 TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA 3001 CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA 3061 GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC 3121 CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC 3181 CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA 3241 GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC 3301 GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC 3361 GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA 3421 CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA 3481 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 3541 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 3601 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA 3661 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA 3721 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT 3781 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG 3841 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA 3901 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA 3961 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG 4021 AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT 4081 GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT 4141 GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG 4201 CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT 4261 TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC 4321 TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA 4381 GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG 4441 CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT 4501 TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG 4561 CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT 4621 CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA 4681 TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG 4741 CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC 4801 CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG 4861 CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG 4921 GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA 4981 AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA 5041 TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC 5101 AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGCA TGACTGGTGG ACAGCAAATG 5161 GGTCGCGGAT CCATGGAAGG AAAGCCAATG CGCAGGTGTA CCAACATTCG ACCAGGAGAG 5221 ACTGGAATGG ATGTAACAAG CCGCTGCACC CTTGGAGACC CCAACAAACT GCCAGAAGGG 5281 GTTCCCCAAC CTGCCCGCAT GCCCTATATC TCAGACAAGC ACCCTCGACA AACCTTGGAA 5341 GTGATTAACC TTCTGAGAAA GCACCGGGAG CTATGTGATG TGGTGCTAGT TGTGGGCGCC 5401 AAGAAGATAT ATGCCCATCG AGTCATTTTG TCAGCCTGTA GTCCCTACTT CCGAGCTATG 5461 TTTACAGGAG AATTGGCAGA GAGCCGTCAG ACAGAAGTAG TGATCCGAGA CATTGACGAG 5521 AGGGCTATGG AATTACTGAT TGACTTTGCG TATACCTCCC AGATAACAGT AGAAGAGGGC 5581 AATGTTCAGA CTCTTCTGCC AGCTGCTTGC CTCCTCCAGC TGGCAGAAAT ACAGGAAGCC 5641 TGCTGTGAAT TCTTAAAGAG ACAATTAGAT CCTTCTAACT GCCTGGGCAT TCGGGCTTTT 5701 GCTGACACAC ATTCATGTCG TGAGTTGCTA AGGATAGCAG ACAAGTTCAC CCAACATAAC 5761 TTTCAAGAGG TAATGGAGAG TGAAGAGTTC ATGTTGCTTC CAGCCAATCA ACTCATTGAT 5821 ATAATATCCA GTGATGAGCT AAACGTTCGC AGTGAAGAAC AAGTGTTCAA TGCAGTGATG 5881 GCCTGGGTCA AATACAGTAT TCAGGAAAGA CGTCCTCAAT TACCCCAGGT GCTGCAGCAT 5941 GTTCGTTTGC CTTTGCTTAG TCCCAAGTTC CTGGTCGGCA CAGTAGGCTC TGATCCCCTC 6001 ATCAAAAGTG ATGAAGAATG CAGAGACTTG GTAGATGAGG CTAAAAACTA CCTCCTATTG 6061 CCGCAAGAAC GACCACTAAT GCAAGGACCA AGGACGAGAC CACGGAAACC TATCCGATGT 6121 GGGGAAGTAC TCTTTGCAGT TGGTGGTTGG TGCAGTGGAG ATGCCATTTC CAGTGTTGAA 6181 CGATATGATC CACAGACCAA TGAATGGAGA ATGGTGGCTT CAATGAGCAA AAGGAGATGC 6241 GGAGTTGGGG TCAGTGTTCT TGATGATCTG TTATATGCAG TAGGAGGCCA TGATGGATCC 6301 TCTTATCTCA ATAGTGTTGA AAGGTATGAC CCCAAAACAA ACCAGTGGAG CAGTGATGTG 6361 GCCCCTACAA GCACCTGCAG GACAAGTGTT GGTGTAGCAG TACTTGGAGG CTTTCTTTAT 6421 GCTGTGGGTG GCCAGGATGG TGTGTCTTGC CTCAACATTG TTGAGAGGTA TGATCCGAAG 6481 GAGAACAAGT GGACTCGGGT AGCTTCTATG AGTACCAGAA GACTAGGTGT GGCTGTGGCT 6541 GTGTTAGGAG GGTTCTTATA TGCTGTAGGT GGCTCTGACG GGACATCTCC TCTCAACACA 6601 GTGGAACGTT ACAATCCTCA GGAAAACAGA TGGCACACTA TAGCCCCTAT GGGGACCCGG 6661 AGGAAACACC TAGGCTGTGC AGTATATCAG GACATGATCT ATGCTGTAGG AGGTAGAGAT 6721 GACACTACAG AGCTGAGCAG TGCTGAGAGA TACAACCCCA GAACCAACCA GTGGTCTCCA 6781 GTGGTGGCCA TGACATCACG CCGTAGTGGA GTTGGCCTGG CAGTGGTCAA TGGACAGCTC 6841 ATGGCAGTAG GAGGTTTTGA TGGCACAACA TACTTGAAGA CCATAGAAGT TTTTGATCCT 6901 GATGCCAATA CATGGAGGTT ATATGGCGGG ATGAATTACC GTCGGCTAGG GGGTGGCGTA 6961 GGAGTTATTA AAATGACACA TTGTGAATCC CATATTTGGC TCGAGCACCA CCACCACCAC 7021 CACTGAGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT 7081 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG 7141 AAAGGAGGAA CTATATCCGG AT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.