pLV3-CMV-Prkaa1-6×His-mCherry-

pLV3-CMV-Prkaa1-6×His-mCherry-Puro plasmid

PVT86181

pLV3-CMV-Prkaa1-6×His-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-Prkaa1(mouse)-6×His-mCherry-Puro
Alias: NM_001013367.3;AMPKalpha1; C130083N04Rik
Gene lenght: 1677bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-CHD1L(human)-6×His-mCherry-Puro
Forward primer:
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Prkaa1-6×His-mCherry-Puro plasmid Description

pLV3-CMV-Prkaa1-6×His-mCherry-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the mouse Prkaa1 gene, promoting the C-terminal fusion expression of the Prkaa1 gene with a 6×His tag, facilitating the affinity interaction of the fusion protein with nickel ions for protein purification. It can also be detected and identified using anti-His antibodies, aiding in the separation, purification, and detection of proteins. The plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid also carries the mCherry red fluorescent protein for detecting the expression and localization of the target gene. Additionally, the plasmid contains a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-Prkaa1-6×His-mCherry-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 1921 TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 1981 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 2041 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 2101 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 2161 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA 2221 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC 2281 TAGAGCTAGC GAATTCGCCA CCATGCGCAG ACTCAGTTCC TGGAGAAAGA TGGCGACGGC 2341 CGAGAAGCAG AAGCACGACG GGCGGGTGAA GATCGGCCAC TACATCCTGG GGGACACGCT 2401 TGGTGTCGGC ACCTTCGGGA AAGTGAAGGT GGGCAAGCAC GAGTTGACCG GACATAAAGT 2461 GGCTGTGAAG ATACTCAACC GGCAGAAGAT TCGGAGCCTT GACGTGGTGG GAAAAATCCG 2521 CCGGGAGATT CAGAACCTGA AGCTGTTCAG GCACCCTCAC ATCATCAAAC TGTACCAGGT 2581 CATCAGTACA CCATCTGATA TTTTCATGGT GATGGAATAT GTCTCTGGAG GAGAGCTATT 2641 TGATTATATC TGTAAAAATG GAAGGTTGGA CGAAAAGGAA AGCCGCCGTC TGTTCCAGCA 2701 GATCCTTTCC GGTGTGGATT ATTGTCACAG GCATATGGTG GTCCACAGAG ATTTGAAACC 2761 TGAGAACGTC CTGCTTGATG CACACATGAA TGCAAAGATA GCCGACTTTG GTCTTTCAAA 2821 CATGATGTCA GATGGTGAAT TTTTAAGAAC AAGCTGTGGC TCACCCAATT ATGCCGCACC 2881 AGAAGTCATT TCAGGAAGAT TGTACGCAGG CCCCGAGGTG GACATCTGGA GCAGCGGGGT 2941 CATTCTCTAT GCTTTGCTGT GTGGAACCCT CCCTTTTGAT GATGACCATG TGCCAACTCT 3001 TTTTAAGAAG ATATGTGATG GGATCTTTTA TACCCCTCAG TACTTAAACC CTTCAGTAAT 3061 CAGCCTTTTG AAACATATGC TGCAGGTGGA TCCCATGAAG AGGGCCGCAA TAAAAGATAT 3121 CAGGGAACAC GAGTGGTTTA AACAGGACCT TCCGAAGTAT CTCTTTCCTG AGGACCCATC 3181 TTATAGTTCA ACCATGATCG ATGACGAAGC CTTGAAAGAA GTGTGTGAGA AGTTCGAGTG 3241 TTCGGAGGAG GAGGTCCTCA GCTGCCTGTA CAACAGAAAC CACCAGGACC CACTAGCCGT 3301 CGCCTACCAC CTCATCATAG ACAACAGGAG AATAATGAAT GAAGCCAAAG ATTTCTACCT 3361 AGCAACCAGC CCACCTGACT CTTTCCTGGA CGACCACCAT TTAACTCGGC CTCACCCTGA 3421 AAGAGTACCG TTCTTGGTTG CCGAAACACC ACGGGCCCGG CACACCCTGG ATGAATTAAA 3481 CCCACAGAAA TCCAAACACC AAGGTGTACG GAAGGCAAAA TGGCATTTGG GAATTCGAAG 3541 TCAAAGCCGA CCCAATGATA TCATGGCAGA AGTTTGTAGA GCAATCAAGC AGTTGGATTA 3601 TGAATGGAAG GTTGTAAACC CCTATTATTT GCGTGTACGA AGGAAGAATC CTGTGACAAG 3661 CACATTTTCC AAAATGAGTC TACAGCTATA CCAAGTGGAT AGTAGGACTT ACTTGTTGGA 3721 TTTCCGTAGT ATTGATGATG AGATTACAGA AGCCAAATCA GGGACTGCTA CTCCACAGAG 3781 ATCGGGATCC ATCAGCAACT ATCGATCTTG CCAAAGGAGT GACTCTGATG CCGAAGCTCA 3841 AGGAAAGCCC TCAGACGTCT CCCTTACCTC ATCTGTCACC TCCCTCGACT CCTCCCCTGT 3901 CGACGTAGCT CCAAGACCAG GAAGTCATAC AATAGAATTT TTTGAAATGT GTGCAAATCT 3961 AATTAAAATT CTTGCACAGG GATCCCATCA TCATCATCAT CACTAGCGGC CGCGAAGGAT 4021 CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA TCGCCCACAG TCCCCGAGAA 4081 GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA AGGTGGCGCG GGGTAAACTG 4141 GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG GGTGGGGGAG AACCGTATAT 4201 AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG TTTGCCGCCA GAACACAGCT 4261 GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC CGCCCTACCT GAGGCCGCCA 4321 TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT GGTGCCTCCT GAACTGCGTC 4381 CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC CTTTGTCCGG CGCTCCCTTG 4441 GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG ACCCTGCTTG CTCAACTCTA 4501 CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC AAGCTGTGAC CGGCGCCTAC 4561 GCTAGACGCC ACCATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT 4621 CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG 4681 CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG 4741 TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC 4801 CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT 4861 CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC 4921 CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC 4981 CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA 5041 CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG 5101 CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG 5161 CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT 5221 GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA 5281 GGGATCTCGC GAGGGCAGAG GAAGTCTTCT AACATGCGGT GACGTGGAGG AGAATCCCGG 5341 CCCTATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC 5401 CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC 5461 GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT 5521 CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC 5581 GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG 5641 CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA 5701 GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT 5761 GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT 5821 CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC 5881 CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC 5941 CTGAAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT 6001 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC 6061 CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA 6121 GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC 6181 CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT 6241 CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG 6301 GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT 6361 GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC 6421 CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG 6481 TCTCCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG CCGCCTCCCC GCCTGGTACC 6541 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 6601 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAAATAAGAT CTGCTTTTTG CTTGTACTGG 6661 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 6721 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 6781 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 6841 TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA 6901 GAGTGAGAGG AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 6961 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 7021 CAATGTATCT TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 7081 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 7141 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGA CTTTTGCAGA 7201 GACGGCCCAA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 7261 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 7321 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 7381 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 7441 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 7501 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 7561 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 7621 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 7681 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 7741 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 7801 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 7861 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 7921 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 7981 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 8041 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 8101 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 8161 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 8221 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 8281 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 8341 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 8401 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 8461 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 8521 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 8581 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 8641 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 8701 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 8761 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 8821 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 8881 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 8941 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 9001 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 9061 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 9121 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 9181 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 9241 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 9301 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 9361 CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT 9421 AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA 9481 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA 9541 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA 9601 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA 9661 AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG 9721 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG 9781 CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT 9841 GCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.