pLV3-CMV-Slc26a2-3×FLAG-CopGFP

pLV3-CMV-Slc26a2-3×FLAG-CopGFP-Puro plasmid

PVT86685

pLV3-CMV-Slc26a2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Slc26a2(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_007885.2;Dtd; ST-OB
Gene lenght: 2217bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Slc26a2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Slc26a2-3×FLAG-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of a 3×FLAG tag, which facilitates detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid carries the CopGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid also carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-Slc26a2-3×FLAG-CopGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 1921 TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 1981 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 2041 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 2101 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 2161 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA 2221 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC 2281 TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGT CTTCAGAAAA TAAAGAGCAG 2341 CATGACCTCT CACCGAGGGA CTTACCTGAA GAAGCCTTTG GTTTCCCATC TGAGCTCCCC 2401 CTGGAGACTC AAAGAAGGTC AGGCACTGAC TTGAGGCAGT CTGAGACCGG TCATGGCCGG 2461 AGAGCTTTCC GTAGGATCCA CATGGAGCTC CGTGAGAAAC CAGACACAGA CATCAAACAG 2521 TTTGTCATCA GAGAGCTCCA GAAGAGTTGC CAGTGCAGCG CAGCCAAAGT CAGAGACGGG 2581 GCTTTTGATT TCTTTCCTGT TTTGCGGTGG CTCCCAAAAT ATGACCTGAA GAAAAACATT 2641 TTAGGTGATG TGATGTCTGG CCTGATTGTG GGTATCCTGT TGGTGCCCCA GTCCATTGCT 2701 TACTCCCTGC TGGCTGGCCA GGAGCCTATA TATGGTCTAT ATACATCATT TTTTGCCAGC 2761 ATTATTTATT TCCTGTTTGG TACATCCCGC CACATCTCTG TGGGCATTTT TGGAATACTG 2821 TGCCTTATGA TTGGTGAGGT TGTTGACCGA GAACTACATA AAGCCTGCCC TGACACTGAT 2881 GCTACGTCAT CTTCGATAGC AGTGTTTTCA AGTGGATGTG TGGTTGTAAA CCATACATTA 2941 GACGGACTCT GTGATAAAAG CTGCTATGCA ATTAAAATTG GCAGCACTGT GACCTTCATG 3001 GCTGGAGTTT ATCAGGTAGC AATGGGCTTC TTTCAAGTGG GCTTTGTCTC TGTCTACCTC 3061 TCCGACGCCT TGCTGAGCGG GTTTGTCACT GGCGCCTCCT TCACCATCCT CACGTCTCAG 3121 GCCAAGTACC TCCTTGGGCT GAGCCTTCCT CGGAGTCATG GTGTCGGCTC AGTCATTACT 3181 ACCTGGATCC ACATCTTCAG AAACATCCGT AACACCAACA TCTGTGATCT CATCACCAGC 3241 CTTTTATGTC TTCTGGTCCT CGTGCCATCC AAAGAACTCA ATGAGCACTT CAAGGACAAG 3301 CTCAAGGCTC CAATTCCGGT CGAGCTCATT GTCGTTGTGG CGGCCACATT GGCTTCTCAT 3361 TTTGGAAAAC TAAATGGGAA TTACAATTCC AGTATTGCTG GACACATTCC CACTGGGTTT 3421 ATGCCACCCA AAGCACCAGA CTGGAGCCTC ATTCCTAATG TGGCTGTAGA TGCAATAGCT 3481 ATTTCTATCA TTGGTTTTGC TATCACTGTA TCACTTTCTG AGATGTTTGC CAAGAAACAT 3541 GGCTACACGG TCAAAGCAAA CCAAGAAATG TATGCCATTG GCTTTTGCAA TATCATACCT 3601 TCCTTCTTCC ACTGCATAAC TACTAGTGCA GCTCTTGCAA AGACATTGGT TAAAGAGTCA 3661 ACAGGCTGCC AGACGCAGCT GTCAGCTATA GTGACAGCCC TTGTCCTTTT GTTGGTCCTT 3721 CTGGTAATAG CTCCTTTATT CTATTCCCTT CAAAAATGTG TCCTTGGTGT GATCACTATT 3781 GTAAATCTCC GGGGCGCACT TCTGAAATTT AGAGACCTGC CAAAGATGTG GAGGCTAAGC 3841 AGAATGGATA CAGTTATCTG GTTTGTGACT ATGCTGTCCT CCGCTCTGTT AAGCACTGAA 3901 ATAGGCCTGC TGGTTGGGGT TTGTTTTTCA ATGTTCTGTG TGATCCTCCG TACTCAGAAG 3961 CCAAAGAATT CACTGCTTGG TTTAGAAGAA GAATCTGAAA CCTTTGAATC CATTTCCACT 4021 TACAAGAACC TTCGGAGCAA GTCCGGCATC AAGGTTTTCC GCTTCATAGC CCCTCTCTAC 4081 TACATAAACA AAGAGTGCTT TAAATCAGCT TTGTACAAGA AAGCTCTTAA CCCAGTCTTG 4141 GTAAAGGCAG CTTGGAAAAA GGCAGCAAAG AGAAAACTCA AAGAAGAAAT GGTGACCTTT 4201 CGTGGGGACC CGGATGAAGT TTCAATGCAG CTTTCCCATG ATCCCTTGGA GGTGCACACT 4261 ATCGTGATCG ACTGTAGTGC CATACAGTTT TTAGACACAG CAGGAATCCA CACACTGAAA 4321 GAAGTTCGCC GGGATTATGA AGCCGTTGGG ATCCAGGTTT TACTGGCGCA GTGCAATCCT 4381 TCTGTGAGGG ATTCCTTGGC CAGAGGAGAA TATTGCAAAA AGGAAGAGGA AACCCTTCTC 4441 TTCTACAGTC TGTCTGAAGC AGTAGCCTTT GCAGAAGACT CTCAGAATCA GAAAGGGGTG 4501 TGTGTTGTCA ACGGTCTGAG TCTCTCTGGT GACGGATCCG ACTACAAAGA CCATGACGGT 4561 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGCG GCCGCGAAGG 4621 ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG 4681 AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC 4741 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT 4801 ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG 4861 CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC GCCGCCCTAC CTGAGGCCGC 4921 CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT GTGGTGCCTC CTGAACTGCG 4981 TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG GCCTTTGTCC GGCGCTCCCT 5041 TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC TGACCCTGCT TGCTCAACTC 5101 TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT CCAAGCTGTG ACCGGCGCCT 5161 ACGCTAGACG CCACCATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC 5221 CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC 5281 CCCAAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC 5341 CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC 5401 GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC 5461 GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC 5521 CGCGTGATCG GCGACTTCAA GGTGGTGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC 5581 ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC 5641 GTGCTGGTGG GCAGCTTCGC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTTC 5701 GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG 5761 GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGCTGCACA GCAACACCGA GCTGGGCATC 5821 GTGGAGTACC AGCACGCCTT CAAGACCCCC ATCGCCTTCG CCAGATCCCG CGCTCAGTCG 5881 TCCAATTCTG CCGTGGACGG CACCGCCGGA CCCGGCTCCA CCGGATCTCG CGAGGGCAGA 5941 GGAAGTCTTC TAACATGCGG TGACGTGGAG GAGAATCCCG GCCCTATGAC CGAGTACAAG 6001 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC 6061 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG 6121 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG 6181 GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG 6241 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG 6301 CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG 6361 GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC 6421 CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC 6481 CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC 6541 GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAAATCA ACCTCTGGAT 6601 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 6661 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 6721 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 6781 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 6841 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 6901 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 6961 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC 7021 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 7081 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 7141 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGTA CCTTTAAGAC CAATGACTTA 7201 CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT 7261 TCACTCCCAA CGAAGATAAG ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA 7321 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 7381 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 7441 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT 7501 TATTATTCAG TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT 7561 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 7621 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 7681 CTGGCTCTAG CTATCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 7741 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 7801 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC 7861 AGAGACCAAA TTCGTAATCA TGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 7921 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 7981 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 8041 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 8101 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 8161 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 8221 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 8281 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 8341 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 8401 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 8461 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 8521 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 8581 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 8641 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 8701 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 8761 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 8821 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 8881 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 8941 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 9001 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 9061 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 9121 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 9181 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 9241 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 9301 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 9361 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 9421 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 9481 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 9541 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 9601 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 9661 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 9721 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 9781 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 9841 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 9901 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 9961 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 10021 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG 10081 GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 10141 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 10201 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 10261 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 10321 GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG 10381 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG 10441 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC 10501 CAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.