pMIGR1-FLAG-RARA-HA-IRES-EGFP

pMIGR1-FLAG-RARA-HA-IRES-EGFP plasmid

PVT86133

pMIGR1-FLAG-RARA-HA-IRES-EGFP plasmid Information

Plasmid name: pMIGR1-FLAG-RARA(human)-HA-IRES-EGFP
Alias: NM_001024809.4;BC071733.1;NR1B1; RAR
Gene lenght: 1371bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: MESC
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pMIGR1-EGFP
Forward primer: PLXSN-F
Reverse primer:
Copy number: High
Induction:

pMIGR1-FLAG-RARA-HA-IRES-EGFP plasmid Description

pMIGR1-FLAG-RARA-HA-IRES-EGFP This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the human RARA gene, promoting the C-terminal fusion expression of the HA tag and the N-terminal fusion expression of the FLAG tag, facilitating experiments such as protein immunoprecipitation and co-immunoprecipitation. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid carries GFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid also carries a Puro resistance gene for selecting host cells expressing the target gene.

pMIGR1-FLAG-RARA-HA-IRES-EGFP plasmid Sequence

1 AGATCTGCCA CCATGGATTA CAAGGACGAC GATGACAAGA TGTACGAGAG TGTAGAAGTG 61 GGGGGTCCCA CCCCTAATCC CTTCCTAGTG GTGGATTTTT ATAACCAGAA CCGGGCCTGT 121 TTGCTCCCAG AGAAGGGGCT CCCCGCCCCG GGTCCGTACT CCACCCCGCT CCGGACTCCG 181 CTTTGGAATG GCTCAAACCA CTCCATTGAG ACCCAGAGCA GCAGTTCTGA AGAGATAGTG 241 CCCAGCCCTC CCTCGCCACC CCCTCTACCC CGCATCTACA AGCCTTGCTT TGTCTGTCAG 301 GACAAGTCCT CAGGCTACCA CTATGGGGTC AGCGCCTGTG AGGGCTGCAA GGGCTTCTTC 361 CGCCGCAGCA TCCAGAAGAA CATGGTGTAC ACGTGTCACC GGGACAAGAA CTGCATCATC 421 AACAAGGTGA CCCGGAACCG CTGCCAGTAC TGCCGACTGC AGAAGTGCTT TGAAGTGGGC 481 ATGTCCAAGG AGTCTGTGAG AAACGACCGA AACAAGAAGA AGAAGGAGGT GCCCAAGCCC 541 GAGTGCTCTG AGAGCTACAC GCTGACGCCG GAGGTGGGGG AGCTCATTGA GAAGGTGCGC 601 AAAGCGCACC AGGAAACCTT CCCTGCCCTC TGCCAGCTGG GCAAATACAC TACGAACAAC 661 AGCTCAGAAC AACGTGTCTC TCTGGACATT GACCTCTGGG ACAAGTTCAG TGAACTCTCC 721 ACCAAGTGCA TCATTAAGAC TGTGGAGTTC GCCAAGCAGC TGCCCGGCTT CACCACCCTC 781 ACCATCGCCG ACCAGATCAC CCTCCTCAAG GCTGCCTGCC TGGACATCCT GATCCTGCGG 841 ATCTGCACGC GGTACACGCC CGAGCAGGAC ACCATGACCT TCTCGGACGG GCTGACCCTG 901 AACCGGACCC AGATGCACAA CGCTGGCTTC GGCCCCCTCA CCGACCTGGT CTTTGCCTTC 961 GCCAACCAGC TGCTGCCCCT GGAGATGGAT GATGCGGAGA CGGGGCTGCT CAGCGCCATC 1021 TGCCTCATCT GCGGAGACCG CCAGGACCTG GAGCAGCCGG ACCGGGTGGA CATGCTGCAG 1081 GAGCCGCTGC TGGAGGCGCT AAAGGTCTAC GTGCGGAAGC GGAGGCCCAG CCGCCCCCAC 1141 ATGTTCCCCA AGATGCTAAT GAAGATTACT GACCTGCGAA GCATCAGCGC CAAGGGGGCT 1201 GAGCGGGTGA TCACGCTGAA GATGGAGATC CCGGGCTCCA TGCCGCCTCT CATCCAGGAA 1261 ATGTTGGAGA ACTCAGAGGG CCTGGACACT CTGAGCGGAC AGCCGGGGGG TGGGGGGCGG 1321 GACGGGGGTG GCCTGGCCCC CCCGCCAGGC AGCTGTAGCC CCAGCCTCAG CCCCAGCTCC 1381 AACAGAAGCA GCCCGGCCAC CCACTCCCCG TACCCATACG ATGTTCCAGA TTACGCTTGA 1441 GAATTCGATT ACAAGGATGA CGACGATAAG GATTACAAGG ATGACGACGA TAAGTAGCAA 1501 TTCCGCCCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT GGAATAAGGC CGGTGTGCGT 1561 TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG CAATGTGAGG GCCCGGAAAC 1621 CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC CCCTCTCGCC AAAGGAATGC 1681 AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA AGCTTCTTGA AGACAAACAA 1741 CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC TGGCGACAGG TGCCTCTGCG 1801 GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC ACAACCCCAG TGCCACGTTG 1861 TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC AAGCGTATTC AACAAGGGGC 1921 TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA TCTGGGGCCT CGGTGCACAT 1981 GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG 2041 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG CAAGGGCGAG 2101 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC 2161 AAGTTCAGCG TGTCTGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG 2221 TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC 2281 TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG 2341 TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC 2401 TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG 2461 AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC 2521 AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGCGAACTTC 2581 AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC 2641 ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC 2701 GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC 2761 GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT AATGAATTAA TTAAGAATTA 2821 TCAAGCTTAT CGATACCGTC GACTAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG 2881 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA 2941 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA 3001 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC 3061 CAAGAACAGA TGGTCCCCAG ATGCGGTCCC GCCCTCAGCA GTTTCTAGAG AACCATCAGA 3121 TGTTTCCAGG GTGCCCCAAG GACCTGAAAA TGACCCTGTG CCTTATTTGA ACTAACCAAT 3181 CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC GAGCTCAATA AAAGAGCCCA 3241 CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATAGACTGCG TCGCCCGGGT ACCCGTGTAT 3301 CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT 3361 CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAG AATTGGTAAT CATGGTCATA 3421 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 3481 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 3541 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 3601 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 3661 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 3721 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 3781 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 3841 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 3901 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 3961 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 4021 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 4081 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 4141 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 4201 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC 4261 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 4321 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 4381 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 4441 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 4501 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 4561 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 4621 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 4681 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 4741 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 4801 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 4861 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 4921 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 4981 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 5041 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 5101 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 5161 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 5221 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 5281 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 5341 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 5401 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 5461 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 5521 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC 5581 CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC 5641 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 5701 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 5761 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 5821 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC 5881 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG 5941 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC 6001 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCACGCTC TCCCTTATGC GACTCCTGCA 6061 TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG 6121 CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC 6181 CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG 6241 CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG 6301 CGTAGAGTGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC 6361 AAGGCATGGA AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA 6421 GACAGCAGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG 6481 GCCAAGAACA GATGGTCCCC AGATGCGGTC CCGCCCTCAG CAGTTTCTAG AGAACCATCA 6541 GATGTTTCCA GGGTGCCCCA AGGACCTGAA AATGACCCTG TGCCTTATTT GAACTAACCA 6601 ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC 6661 CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATAGACTG CGTCGCCCGG GTACCCGTAT 6721 TCCCAATAAA GCCTCTTGCT GTTTGCATCC GAATCGTGGA CTCGCTGATC CTTGGGAGGG 6781 TCTCCTCAGA TTGATTGACT GCCCACCTCG GGGGTCTTTC ATTTGGAGGT TCCACCGAGA 6841 TTTGGAGACC CCTGCCTAGG GACCACCGAC CCCCCCGCCG GGAGGTAAGC TGGCCAGCGG 6901 TCGTTTCGTG TCTGTCTCTG TCTTTGTGCG TGTTTGTGCC GGCATCTAAT GTTTGCGCCT 6961 GCGTCTGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG GAACTGACGA 7021 GTTCTGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTTGGGG GCCGTTTTTG 7081 TGGCCCGACC TGAGGAAGGG AGTCGATGTG GAATCCGACC CCGTCAGGAT ATGTGGTTCT 7141 GGTAGGAGAC GAGAACCTAA AACAGTTCCC GCCTCCGTCT GAATTTTTGC TTTCGGTTTG 7201 GAACCGAAGC CGCGCGTCTT GTCTGCTGCA GCGCTGCAGC ATCGTTCTGT GTTGTCTCTG 7261 TCTGACTGTG TTTCTGTATT TGTCTGAAAA TTAGGGCCAG ACTGTTACCA CTCCCTTAAG 7321 TTTGACCTTA GGTCACTGGA AAGATGTCGA GCGGATCGCT CACAACCAGT CGGTAGATGT 7381 CAAGAAGAGA CGTTGGGTTA CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG 7441 GCCGCGAGAC GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC 7501 ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG AAGCCTTGGC 7561 TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT AAGCCTCCGC CTCCTCTTCC 7621 TCCATCCGCC CCGTCTCTCC CCCTTGAACC TCCTCGTTCG ACCCCGCCTC GATCCTCCCT 7681 TTATCCAGCC CTCACTCCTT CTCTAGGCGC CGGAATT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.