Monarch® 磁珠法 PCR & DNA 纯化试剂盒（5

品牌	货号	产品中文名称	产品英文名称
NEB	T4130	Monarch® 磁珠法 PCR & DNA 纯化试剂盒（5 μg）	Monarch®Mag PCR & DNA Cleanup Kit (5 μg)

货号	产品中文名称	产品英文名称	规格
T4130L	Monarch® 磁珠法 PCR & DNA 纯化试剂盒（5 μg）	Monarch Mag PCR & DNA Cleanup Kit (5 µg)	400 preps
T4130S	Monarch® 磁珠法 PCR & DNA 纯化试剂盒（5 μg）	Monarch Mag PCR & DNA Cleanup Kit (5 µg)	100 preps
T4130V	Monarch® 磁珠法 PCR & DNA 纯化试剂盒（5 μg）	Monarch Mag PCR & DNA Cleanup Kit (5 µg)	10 preps

产品特点

• Magnetic bead-based kit for reliable, high-throughput PCR and DNA cleanup and purification
• Purify up to 5 μg of DNA using a standard bind-wash-elute workflow
• Compatible with manual and automated systems to support workflow flexibility
• No pH monitoring needed, with our optimized buffer chemistry
• Modified protocols available for purifying oligonucleotides, genomic DNA, and RCA products
• Additional protocol supports varying DNA binding capacities and sample volumes

The Monarch Mag PCR & DNA Cleanup Kit (5 μg) enables rapid and reliable purification and concentration of up to 5 μg of DNA, including both single- and double- stranded forms, from common enzymatic reactions such as PCR, restriction digestion, ligation, reverse transcription, and rolling circle amplification (RCA). Using a streamlined bind-wash-elute workflow with silica-coated magnetic beads, the kit delivers highly pure, inhibitor-free DNA ready for immediate use in downstream applications such as in vitro transcription (IVT), sequencing, restriction digestion, library construction, labeling, and other enzymatic manipulations. The magnetic bead format offers flexibility and scalability, supporting both manual and automated workflows. Additional modified protocols support varying DNA input amounts and sample volumes and enable purification of oligos, genomic DNA, and RCA products.

The kit includes magnetic beads, optimized buffers, and reagents, all compactly packaged. The magnetic bead technology in this kit eliminates the need for centrifugation or vacuum-based processing and requires no pH monitoring, with the optimized buffer chemistry.

Monarch kits are manufactured to ensure high quality and consistency. As with all Monarch kits, this product is designed with sustainability in mind, reducing plastic usage where possible and in the design of buffer bottles and other plastic components, while maintaining performance and reliability across a wide range of applications.

Properties
Purification Format	Magnetic bead
DNA Sample Type Compatibility	DNA from various enzymatic reactions (restriction digestion, ligation, reverse transcription, PCR, RCA, etc.)
	dsDNA, ssDNA, circular/linear DNA, oligonucleotides, gDNA, and RCA
Typical Recovery	70 - 90%
DNA Purity	A260/280 > 1.8 and A260/230 > 1.8
Binding Capacity	Up to 5 µg using a standard protocol. The binding capacity can be scaled up or down as needed to accommodate different sample requirements.
Elution Volume	25–50 µl
DNA Size Range	Standard protocol: 50 bp – 25 kb
	Oligonucleotide Cleanup protocol: ssDNA ≥18 nt and dsDNA ≥14 nt
	gDNA cleanup protocol: ≥25 kb
Compatible Downstream Applications	Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing, in vitro transcription template

备注：以上仅为 T4130 的部分产品信息，如需NEB货号 T4130 的完整产品信息及相关的实验操作手册等等，请通过NEB官网查找对应的货号获取。