相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
| 品牌 | 货号 | 产品中文名称 | 产品英文名称 |
|---|---|---|---|
| NEB | P7719 | 彩色预染蛋白 Standard,宽范围(10-250 kDa) | Color Prestained Protein Standard, Broad Range (10-250 kDa) |
| 货号 | 产品中文名称 | 产品英文名称 | 规格 |
|---|---|---|---|
| P7719L | 彩色预染蛋白 Standard,宽范围(10-250 kDa) | Color Prestained Protein Standard, Broad Range (10-250 kDa) | 750 gel lanes |
| P7719S | 彩色预染蛋白 Standard,宽范围(10-250 kDa) | Color Prestained Protein Standard, Broad Range (10-250 kDa) | 150 gel lanes |
产品特点
The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed.
- Allows approximate molecular weight determination when performing SDS-PAGE analysis.
- Applications include verification of Western transfer efficiency on membranes and fluorescent imaging of SDS-PAGE.
- Direct loading, additional loading buffer and heat incubation not required
- Recombinant proteins with no detectable protease contaminating activities
- Optimal stability for up to 24 months

The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed. The Color Prestained Protein Standard is designed for observing protein separation during SDS-PAGE, verification of western transfer efficiency on membranes, and for approximating the size of proteins (1-2). The Color Prestained Protein Standard may also be used in fluorescent imaging of SDS-PAGE.
备注:以上仅为 P7719 的部分产品信息,如需NEB货号 P7719 的完整产品信息及相关的实验操作手册等等,请通过NEB官网查找对应的货号获取。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验效率对WB结果的影响都问题不大 (300KDa蛋白如何,没有尝试过)。 电泳一般采用恒流,45mA-55mA(应根据电泳仪器适当调整,要注意仪器最高限压;此条件适合gibco modelV16型,胶宽20cm)。采用恒流的优点,保证最快速度完成电泳(电压会逐渐增大),省时且减少扩散;但是由于电泳速度过快时会发热而溶胶,所以你必须想办法散热。散热不好,条带出现波浪状。 注意事项: 1.聚丙烯酰胺的30%母液会降解,要4度避光保存。 2.APS会失效,10%APS一般保质期才一个
Buffer) 用于PCR的标准缓冲液见PCR操作范例。于72℃时,反应体系的pH值将下降1个单位,接近于7.2。二价阳离子的存在至关重要,影响PCR的特异性和产量。实验表明,Mg2+ 优于Mn2+ ,而Ca2+ 无任何作用。 1.Mg2+ 浓度Mg2+ 的最佳浓度为1.5mmol/L(当各种dNTP浓度为200mmol/L时),但并非对任何一种模板与引物的结合都是最佳的。首次使用靶序列和引物结合时,都要把Mg2+ 浓度调到最佳,其浓度变化范围为1~10mmol/L。Mg2+ 过量易生
.ncbi.nlm.nih.gov/UniGene/ Non-redundant, gene-oriented clusters l Comparative Genomics 10. Clusters of Orthologous Groups (COG) http://www.ncbi.nlm.nih.gov/COG Phylogenetic classification of proteins from 43 complete genomes 11. CORG http
技术资料暂无技术资料 索取技术资料







