HiScribe® T7 ARCA mRNA 试剂盒 | N

品牌	货号	产品中文名称	产品英文名称
NEB	E2065	HiScribe® T7 ARCA mRNA 试剂盒	HiScribe®T7 ARCA mRNA Kit

货号	产品中文名称	产品英文名称	规格
E2065S	HiScribe® T7 ARCA mRNA 试剂盒	HiScribe T7 ARCA mRNA Kit	20 reactions

产品特点

• 单次反应可产生多达 20 μg 加帽 mRNA
• 2 小时内完成 mRNA 加帽、DNA 去除、mRNA 加尾和纯化。同类产品需要更多的移液步骤和独立组分。
• 可部分掺入 5mCTP、Pseudo-UTP 及其它经修饰的 CTP 和 UTP
• 超高品质组分确保 mRNA 完整性
• 基于 ARCA 的正确方向加帽确保了高翻译效率
• 包含模板去除和 mRNA 纯化试剂
• HiScribe^®; 试剂盒的反应次数是同类产品的两倍
• 也提供含加尾试剂的产品

Most eukaryotic mRNAs require a 7-methyl guanosine (m7G) cap structure at the 5´ end and a Poly(A) tail at the 3´ end to be efficiently translated. By using a DNA template encoding a poly(A) tail, the HiScribe T7 ARCA mRNA Kit can be used to synthesize capped and tailed mRNAs. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA) (NEB #S1411) using T7 RNA Polymerase. Poly(A) tail is incorporated during the transcription reaction. The kit also includes DNase I and LiCl for DNA template removal and quick mRNA purification.
Additionally, the kit is capable of partial incorporation of modified UTP and CTP (up to 50% each) without affecting the mRNA yield significantly. By using a DNA template encoding a poly(A) tail, capped and tailed modified mRNA can be synthesized in a single reaction in 30 minutes. mRNAs synthesized with the kit can be used for cell transfection, microinjection, in vitro translation and RNA vaccines.
ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated. Standard cap analogs can be incorporated in either direction resulting in only 50% of capped mRNA that is functional in protein translation.
Figure 1: Structure of Anti-Reverse Cap Analog (ARCA, NEB #S1411)

Methylation at the 3´ position of 7mG forces the cap structure to be attached to mRNA in the correct orientation.
Figure 2: T7 RNA Polymerase co-transcriptionally caps RNA with anti-reverse cap analog (ARCA)

Figure 3: Overview of mRNA synthesis workflow with the HiScribe T7 ARCA mRNA Kit

产品类别:

RNA 加帽,

RNA 合成体外转录（IVT）

备注：以上仅为 E2065 的部分产品信息，如需NEB货号 E2065 的完整产品信息及相关的实验操作手册等等，请通过NEB官网查找对应的货号获取。