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10 次
Code No. 3288
| Human Cell-Free Protein Expression Master Mix for mRNA | 180 μl(10次反应量) |
Human Cell-Free Protein Expression Master Mix for mRNA是使用mRNA进行无细胞蛋白质合成的试剂。本制品含有人细胞来源的核糖体、翻译起始/延伸因子和tRNA等所有蛋白质合成的必需因子。只需在一个反应管中向本制品添加编码蛋白质的加帽mRNA*1,在32℃下孵育数小时即可完成蛋白质合成的反应,操作十分简单。与传统的基于细胞的实验相比,本制品不仅是目标蛋白质生产的快速方法,也是mRNA构建体评估的快速方法。因此,适用于筛选疫苗开发等应用的mRNA构建体。
* 1:蛋白质表达需要在mRNA上添加帽子结构。一般情况下,可使用帽类似物进行共转录加帽或者使用加帽酶,比如Vaccinia virus或Faustovirus来源的加帽酶,以及2’-O-Methyltransferase。
-80℃
※收到后,请立即在-80℃下保存。将该制品置于-80℃以上可能导致性能下降。
※如果不能一次用完,建议在第一次融化时分装保存,以避免反复冻融导致性能下降。
1. mRNA构建体设计的筛选
2. 无细胞合成毒性蛋白质
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文献和实验An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources
-untranslated region (UTR) sequence from a baculovirus polyhedrin gene as a translational enhancer, was also developed to obtain maximum performance from the insect cell-free protein synthesis system. This combination of insect cell extract and expression vector
Advances in Genome-Wide Protein Expression Using the Wheat Germ Cell-Free System
, and when programmed with any mRNA in the presence of energy sources and amino acids, can synthesize the respective protein in vitro. Among the cell-free systems, the wheat germ-based translation system is of special interest due to its eukaryotic nature and robustness
Expression of cDNA-Encoded Proteins by Cell-Free Transcription and Translation
, and does not need to be physically handled itself to permit the recovery of cognate cDNA clones; in this way, this approach differs radically from strategies based on microscale sequencing. However, other investigators may wish to use cell-free expression to monitor mutagenesis
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