2L VACUUM FLASK W/CONICAL BOTT

Dropout plates for yeast

of a 10x master solution: Grab a 500 ml conical flask Figure out how much of your dropout powder you''re going to need and weigh it out For example, the CSM - TRP mix we have is 0.74g/L, so for a 200ml of a 10x solution

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture

Southern Analysis

of 0.1 M Tris, pH 7.6 to neutralize (20 min on rotator).Pour off and add 900 ml of 20 x SSC for 45min with gentle rotation.To keep gel immersed during above steps, can balance an inverted ddH2O filled 50ml conical on it, making sure that the top surface