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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Staurosporine
- 供应商:
上海汇谱仪器有限公司
- CAS号:
459130-BR
- 规格:
500 µg
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文献和实验with any kinase inhibitor as selectivity function can be synthesized. We here used staurosporine as the selectivity function because it targets and, therefore, allows profiling a broad range of kinases (Romano and Giordano, Cell Cycle 7:3364–3668, 2008
原代心肌细胞的培养及实验方案 取出生后0~24 h C57BL/6cr小鼠心脏,按改良的Lader方法进行细胞的分离。 获取博动的心脏迅速放置于无Ca2+、Mg2+的Hank’s平衡液中。剪碎心脏,放置在4℃条件下100 g/L的Trypsin溶液中消化16~18 h,用Trypsin抑制剂中止消化。然后在37℃下,用胶原酶Ⅱ于CO2培养箱内,在摇床上继续孵化消化45~60 min,最后用70 μm直径尼龙过滤网过滤获取心肌细胞,用含有25 mmol/L HCO3-,100 mL/L
(GIBCO-BRL) according to the manufacturer's recommendations. Following transfection, the cells were allowed to recover in complete medium for 16 h and were then either left untreated or an apoptotic stimulus (800 ng/ml staurosporine; Sigma) (22 ,23
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