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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 检测范围:
31.25 pg/mL-2,000 pg/mL
- 检测方法:
夹心法
- 应用:
ELISA检测
- 适应物种:
小鼠
- 样本:
细胞培养上清#血清#血浆
- 灵敏度:
31.25 pg/mL
- 规格:
48 T/96 T
| 规格: | 48 T | 产品价格: | ¥1598.0 |
|---|---|---|---|
| 规格: | 96 T | 产品价格: | ¥2558.0 |
产品概述:EliKine™ 小鼠脂联素(Adiponectin) ELISA试剂盒
产品名称:脂联素酶联免疫试剂盒(小鼠) | 货号:KTE3079-3
产品类型:夹心法ELISA核心试剂盒 | 反应性:特异性针对小鼠样本
主要应用:精准定量检测小鼠血清、血浆及细胞培养上清液中的脂联素(Adiponectin)蛋白水平。
核心特性与检测性能
- 高灵敏度检测:最低检测浓度可达 31.25 pg/mL,能够有效识别样本中微量的脂联素。
- 宽广的线性范围:检测范围为 31.25 pg/mL - 2,000 pg/mL,适用于不同浓度水平的样本分析。
- 出色的特异性:基于EliKine™ 技术平台,该试剂盒对目标蛋白表现出高特异性,经测试与类似物无明显交叉反应或背景干扰。
试剂盒组分(完整配置)
- 预包被微孔板 (Mouse Adiponectin Microplate)
- 脂联素标准品 (冻干粉) (Mouse Adiponectin Standard)
- 10× Assay Buffer
- 100× 检测抗体 (Mouse Adiponectin Detect Antibody)
- 100× Streptavidin-HRP 偶联物
- HRP 显色底物 (TMB)
- 终止液 (Stop Solution)
- 20× 洗涤缓冲液 (Wash Buffer)
- 板盖 (Plate Covers)
背景介绍与研究意义
脂联素(Adiponectin/Acrp30)是一种由脂肪细胞特异性分泌的蛋白质激素,在血浆中含量丰富。它在调节葡萄糖稳态、脂肪酸氧化以及能量代谢中扮演着关键角色。研究证实,脂联素具有抗动脉粥样硬化、抗炎等重要生理功能,其血清水平的变化与2型糖尿病、肥胖、非酒精性脂肪肝等多种代谢性疾病密切相关。本试剂盒为研究小鼠模型中脂联素的生物学功能及其在代谢疾病中的作用提供了可靠的定量分析工具。
实验操作与保存指南
样本类型:适用于小鼠来源的血清、血浆及细胞培养上清液。
保存条件:未开封试剂盒请避光保存于2-8℃,保质期24个月。启用后,请参照说明书分别保存各组分。
运输条件:全程采用冰袋(蓝冰)低温运输,确保试剂活性。
重要注意事项
- 请勿混合使用不同批号或已过有效期的试剂。
- 使用前,所有试剂需室温平衡约30分钟。
- 为保障结果准确性,建议对样品和标准品进行复孔(双孔或三孔)检测。
- 实验过程中请使用一次性耗材,并确保充分混匀反应体系。
规格与价格
| 规格 | 价格 (人民币) | 货期 |
|---|---|---|
| 48 T | 1598.0 | 请咨询 |
| 96 T | 2558.0 | 请咨询 |
警告: 本产品仅供科学研究使用,不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Product Overview: EliKine™ Mouse Adiponectin ELISA Kit
Product Name: Adiponectin Enzyme-Linked Immunosorbent Assay Kit (Mouse) | Catalog Number: KTE3079-3
Product Type: Sandwich ELISA Core Kit | Reactivity: Specific for mouse samples
Primary Application: For the precise quantitative detection of Adiponectin protein levels in mouse serum, plasma, and cell culture supernatants.
Core Features & Assay Performance
- High Sensitivity Detection: Minimum detectable concentration reaches 31.25 pg/mL, enabling effective identification of trace amounts of Adiponectin in samples.
- Broad Linear Range: Detection range is 31.25 pg/mL - 2,000 pg/mL, suitable for analyzing samples with varying concentration levels.
- Excellent Specificity: Based on the EliKine™ technology platform, this kit demonstrates high specificity for the target protein, with tested negligible cross-reactivity or background interference with analogues.
Kit Components (Complete Configuration)
- Pre-coated Microplate (Mouse Adiponectin Microplate)
- Adiponectin Standard (Lyophilized Powder) (Mouse Adiponectin Standard)
- 10× Assay Buffer
- 100× Detection Antibody (Mouse Adiponectin Detect Antibody)
- 100× Streptavidin-HRP Conjugate
- HRP Color Development Substrate (TMB)
- Stop Solution
- 20× Wash Buffer
- Plate Covers
Background & Research Significance
Adiponectin (Adiponectin/Acrp30) is a protein hormone specifically secreted by adipocytes and is abundant in plasma. It plays a key role in regulating glucose homeostasis, fatty acid oxidation, and energy metabolism. Research has confirmed that Adiponectin possesses important physiological functions such as anti-atherosclerotic and anti-inflammatory effects. Changes in its serum levels are closely associated with various metabolic diseases including type 2 diabetes, obesity, and non-alcoholic fatty liver disease. This kit provides a reliable quantitative analysis tool for studying the biological functions of Adiponectin in mouse models and its role in metabolic diseases.
Experimental Procedure & Storage Guidelines
Sample Types: Suitable for mouse-derived serum, plasma, and cell culture supernatants.
Storage Conditions: Store the unopened kit protected from light at 2-8°C; shelf life is 24 months. After opening, store individual components separately according to the instructions.
Shipping Conditions: Shipped with ice packs (blue ice) throughout to ensure reagent activity.
Important Notes
- Do not mix reagents from different lot numbers or expired reagents.
- All reagents should be equilibrated to room temperature for approximately 30 minutes before use.
- For accurate results, it is recommended to test samples and standards in duplicate or triplicate.
- Use disposable consumables during the experiment and ensure thorough mixing of the reaction system.
Specifications & Pricing
| Specification | Price (RMB) | Lead Time |
|---|---|---|
| 48 T | 1598.0 | Please inquire |
| 96 T | 2558.0 | Please inquire |
Warning: This product is for research use only. Not intended for human or clinical diagnostic use. Users should ensure their application does not violate any patents or license agreements.
Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.
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