IgG4/IGHG4 ELISA KIT 免疫球蛋白G4EL

EliKine™ 小鼠免疫球蛋白G4 (IgG4) ELISA试剂盒 (一步法) - 货号 KTE4014-2

本试剂盒专为精准定量检测小鼠血清、血浆、组织匀浆及细胞上清液等样本中的免疫球蛋白G4 (IgG4) 含量而设计。作为核心产品，它采用先进的双抗体夹心一步法原理，为生命科学研究与生物制药质控提供可靠的分析工具。

产品核心信息

• 产品名称：IgG4/IGHG4 ELISA KIT 免疫球蛋白G4 ELISA试剂盒(小鼠)
• 货号：KTE4014-2
• 反应性：小鼠
• 检测方法：双抗体夹心ELISA (一步法)
• 样本类型：血清、血浆、组织、细胞上清及相关液体样本
• 检测范围：50 pg/mL - 4000 pg/mL
• 灵敏度：10 pg/mL

产品特点与优势

EliKine™ ELISA系列试剂盒以其卓越的性能著称。本试剂盒经过优化，对目标蛋白IgG4表现出极高的灵敏度和特异性。严格的交叉反应测试表明，对目标蛋白的类似物检测，尚未发现明显的交叉反应或背景干扰，确保了实验数据的准确性与可靠性。

试剂盒组分 (以96T规格为例)

• Mouse IgG4 预包被微孔板
• Mouse IgG4 标准品 (8,000 pg/mL)
• 标准品稀释液
• Mouse IgG4 检测抗体 (生物素标记)
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP 底物A / HRP 底物B (TMB)
• 终止液
• 洗涤缓冲液 (浓缩液)
• 板盖

注：48T规格试剂盒组分相应减少。

技术规格

规格	价格 (人民币)	货期
48 T	1,998.0	期货 (预计3个工作日内发货)
96 T	3,198.0	期货 (预计3个工作日内发货)

检测原理简介

本试剂盒采用双抗体夹心ELISA法。预包被于微孔板上的特异性抗体捕获样本中的小鼠IgG4，随后依次加入生物素化的检测抗体和链霉亲和素-HRP，形成免疫复合物。加入TMB底物显色，颜色的深浅与样本中小鼠IgG4的浓度成正比，通过酶标仪在450 nm波长下测定吸光度(OD值)即可进行定量分析。

重要注意事项

• 请勿混合使用不同批号或已过期的试剂。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 充分的混匀对结果至关重要，建议使用微量振荡器或定期手动摇匀。
• 为保证结果精度，推荐对样品和标准品进行复孔检测（双孔或三孔）。

保存与运输

• 保存建议：未开封的试剂盒请避光保存于2-8℃，有效期6个月。启用后，请参照说明书分别保存各组分。
• 运输条件：全程蓝冰（冰袋）运输，以确保试剂活性。

警告： 本产品仅供科研使用，不适用于人类或临床诊断。使用者应确保其应用不违反相关专利或许可规定。对于因使用本产品可能导致的专利侵权或其他违规行为，制造商不承担任何责任。

别名信息

Immunoglobulin G4; IgG4; IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Immunoglobulin G4 (IgG4) ELISA Kit (One-Step) - Cat. No. KTE4014-2

This kit is specifically designed for the precise quantitative detection of Immunoglobulin G4 (IgG4) levels in samples such as mouse serum, plasma, tissue homogenates, and cell culture supernatants. As a core product, it utilizes the advanced double-antibody sandwich one-step method, providing a reliable analytical tool for life science research and biopharmaceutical quality control.

Core Product Information

• Product Name: IgG4/IGHG4 ELISA KIT Immunoglobulin G4 ELISA Kit (Mouse)
• Catalog Number: KTE4014-2
• Reactivity: Mouse
• Detection Method: Double-Antibody Sandwich ELISA (One-Step)
• Sample Types: Serum, Plasma, Tissue, Cell Supernatant and related liquid samples
• Detection Range: 50 pg/mL - 4000 pg/mL
• Sensitivity: 10 pg/mL

Features and Advantages

The EliKine™ ELISA series kits are renowned for their excellent performance. This kit is optimized to exhibit high sensitivity and specificity towards the target protein, IgG4. Rigorous cross-reactivity tests indicate no significant cross-reactivity or background interference with analogs of the target protein, ensuring the accuracy and reliability of experimental data.

Kit Components (Example for 96T)

• Mouse IgG4 Pre-coated Microplate
• Mouse IgG4 Standard (8,000 pg/mL)
• Standard Diluent
• Mouse IgG4 Detection Antibody (Biotin-labeled)
• Streptavidin-HRP
• HRP Substrate A / HRP Substrate B (TMB)
• Stop Solution
• Wash Buffer (Concentrate)
• Plate Sealer

Note: Components are proportionally reduced for the 48T kit size.

Technical Specifications

Size	Price (RMB)	Lead Time
48 T	1,998.0	Future Goods (Estimated shipment within 3 business days)
96 T	3,198.0	Future Goods (Estimated shipment within 3 business days)

Detection Principle Overview

This kit employs the double-antibody sandwich ELISA method. The specific antibody pre-coated onto the microplate captures mouse IgG4 from the sample. Subsequently, the biotinylated detection antibody and Streptavidin-HRP are added sequentially to form an immune complex. After adding the TMB substrate for color development, the color intensity is proportional to the concentration of mouse IgG4 in the sample. Quantitative analysis is performed by measuring the absorbance (OD value) at 450 nm using a microplate reader.

Important Notes

• Do not mix reagents from different lots or use expired reagents.
• All reagents should be equilibrated to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the procedure to avoid cross-contamination.
• Unused strips should be returned to the original foil pouch immediately and sealed for storage at 4°C.
• Thorough mixing is crucial for accurate results; using a microplate shaker or periodic manual shaking is recommended.
• For optimal precision, it is recommended to perform duplicate or triplicate measurements for both samples and standards.

Storage and Shipping

• Storage Recommendation: Store the unopened kit protected from light at 2-8°C; it is stable for 6 months. After opening, store individual components according to the instructions.
• Shipping Conditions: Shipped with blue ice (ice packs) throughout to ensure reagent activity.

Warning: This product is for research use only. It is not intended for human or clinical diagnostic use. The user is responsible for ensuring that its application does not infringe relevant patents or licensing regulations. The manufacturer assumes no liability for patent infringement or other violations that may result from the use of this product.

Alias Information

Immunoglobulin G4; IgG4; IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yaken Company.