IL-2 ELISA KIT 白介素-2ELISA试剂盒(小

产品概述

货号：KTE7006-2

产品名称：EliKine™ 小鼠白介素-2 (IL-2) ELISA试剂盒 (Mouse IL-2 ELISA Kit)

本试剂盒是一款基于经典夹心法原理的高灵敏度ELISA检测产品，专为精准定量小鼠来源样本（如细胞培养上清、血清、血浆等）中的白介素-2 (IL-2) 蛋白水平而设计。作为细胞分析领域的核心产品，它为您研究免疫调控、T细胞克隆扩增等关键生物学过程提供了可靠的工具。

主要特点与优势

• 高灵敏度与特异性：检测灵敏度达150 pg/mL，对目标蛋白类似物无明显交叉反应或背景干扰，确保结果的准确性。
• 优化的检测范围：线性检测范围为200 pg/mL - 3000 pg/mL，覆盖广泛的生理和病理条件下的小鼠IL-2浓度。
• 即用型组分：试剂盒包含经过优化的预包被板、标准品、检测抗体及全套检测试剂，操作流程简便，节省实验优化时间。
• 稳定的性能：采用优质的EliKine™ Streptavidin-HRP体系，信号稳定，重复性好。

产品详细信息

反应性：小鼠

样本类型：细胞培养上清、血清、血浆及其它生物液体

检测方法：夹心法ELISA

试剂盒组分（详细列表）

• 小鼠源IL-2抗体预包被微孔板
• 小鼠源IL-2蛋白标准品
• 小鼠源IL-2检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液
• 实验缓冲液
• HRP底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

背景信息

白细胞介素-2 (IL-2) 是一种分子量约为15 kDa的糖蛋白，是免疫系统中的关键细胞因子，主要由激活的T细胞产生。它通过调控淋巴细胞（尤其是T细胞）的活性、增殖和分化，在机体对抗微生物感染、区分外来与自身抗原的免疫应答中扮演核心角色。小鼠IL-2的基因ID为16183。

规格与价格

规格	价格 (元)	货期
48 T	1598.0	现货（次日发货）
96 T	2558.0	现货（次日发货）

保存与运输

保存建议：请将未开封的试剂盒储存于2-8℃。启用后，请参照说明书妥善保存各组分。

运输条件：冰袋（蓝冰）运输。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条需立即放回原包装并密封，于4℃保存。
• 充分混匀试剂对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对样品和标准品进行复孔（双孔或三孔）检测。

警告：本产品仅供研究使用，不适用于人类或临床诊断。

相关链接： 小鼠IL-2 (P04351) 蛋白序列信息可参考：UniProtKB - P04351

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Catalog No.：KTE7006-2

Product Name：EliKine™ Mouse Interleukin-2 (IL-2) ELISA Kit (Mouse IL-2 ELISA Kit)

This kit is a high‑sensitivity ELISA assay based on the classic sandwich principle, specifically designed for the precise quantification of interleukin‑2 (IL‑2) protein levels in mouse‑derived samples (e.g., cell culture supernatants, serum, plasma, etc.). As a core product in cellular analysis, it provides a reliable tool for investigating immune regulation, T‑cell clonal expansion, and other critical biological processes.

Key Features and Advantages

• High Sensitivity and Specificity：Detection limit of 150 pg/mL, with negligible cross‑reactivity or background interference from related proteins, ensuring accurate results.
• Optimized Detection Range：Linear range of 200 pg/mL – 3000 pg/mL, covering a broad spectrum of physiological and pathological mouse IL‑2 concentrations.
• Ready‑to‑Use Components：The kit includes optimized pre‑coated plates, standards, detection antibody, and a complete set of reagents, simplifying workflow and saving assay development time.
• Robust Performance：Utilizes high‑quality EliKine™ Streptavidin‑HRP system, providing stable signal and excellent repeatability.

Product Specifications

Reactivity：Mouse

Sample Types：Cell culture supernatants, serum, plasma, and other biological fluids

Assay Method：Sandwich ELISA

Kit Components (Detailed List)

• Mouse IL‑2 Antibody Pre‑coated Microplate
• Mouse IL‑2 Protein Standard
• Mouse IL‑2 Detection Antibody
• EliKine™ Streptavidin‑HRP
• Standard Diluent
• Assay Buffer
• HRP Substrate
• Stop Solution
• Wash Buffer
• Plate Sealing Film

Background Information

Interleukin‑2 (IL‑2) is a ~15 kDa glycoprotein that serves as a pivotal cytokine in the immune system, primarily secreted by activated T cells. By regulating lymphocyte (especially T‑cell) activity, proliferation, and differentiation, IL‑2 plays a central role in host defense against microbial infections and in discriminating foreign from self antigens. The mouse IL‑2 gene ID is 16183.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T	1598.0	In stock (next‑day shipment)
96 T	2558.0	In stock (next‑day shipment)

Storage and Shipping

Storage Recommendation：Store unopened kits at 2–8 °C. After opening, refer to the user manual for proper storage of individual components.

Shipping Conditions：Transport on ice packs (blue ice).

Critical Precautions

• Do not mix reagents from different lot numbers or past their expiration date.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the assay to avoid cross‑contamination.
• Unused strips should be immediately returned to their original packaging, sealed, and stored at 4 °C.
• Thorough mixing of reagents is critical for reliable results; use a low‑speed vortex or periodically invert manually.
• For greater data reliability, assay samples and standards in replicate wells (duplicate or triplicate).

Warning：This product is for research use only and is not intended for human or clinical diagnostic applications.

Related Link：Mouse IL‑2 (P04351) protein sequence information can be found at: UniProtKB - P04351

Note: The above information is subject to change due to product optimization or updates; please contact Yakein Biotechnology for the latest details.