小鼠脂联素 ELISA定量试剂盒(Adiponectin)

小鼠脂联素 ELISA 定量试剂盒 (Mouse Adiponectin ELISA Kit) – KTE3079-1

产品概述

本套件为EliKine™ 核心产品，专为细胞分析与ELISA 应用设计，采用双抗体夹心法对小鼠脂联素（Adiponectin）进行高灵敏度定量检测。适用于细胞培养上清、血清及血浆样本，帮助科研人员在代谢、炎症及心血管疾病研究中获取可靠的数据支持。

主要技术参数

项目	内容
检测类型	ELISA（夹心法）
反应物种	小鼠
检测范围	31.25 pg/mL – 2,000 pg/mL
检测灵敏度	31.25 pg/mL
样本类型	细胞培养上清、血清、血浆
货号	KTE3079-1
保存条件（未开封）	2 ℃ – 8 ℃，避光，保质期 24 个月
运输方式	冰袋（蓝冰）

试剂盒组成

• Mouse Adiponectin Microplate
• Mouse Adiponectin Standard（冻干）
• Assay Buffer (10×)
• Mouse Adiponectin Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

特点 & 优势

• 极高的灵敏度（31.25 pg/mL）和宽检测范围。
• 特异性抗体对脂联素同系物的交叉反应极低，背景噪声可控。
• 采用专有链霉亲和素‑HRP复合物，提升信号强度。
• 适用于单孔、双孔或三孔重复检测，提高实验可重复性。

使用注意事项

• 试剂仅限同批次使用，严禁混用不同批号或过期试剂。
• 使用前请在室温（约20‑25 ℃）平衡30 分钟。
• 实验操作建议使用一次性吸头，防止交叉污染。
• 未使用的板条应立即放回包装，密封保存于4 ℃。
• 每10 分钟轻轻摇匀一次（推荐低速振荡器），确保反应均匀。
• 样品与标准建议做双孔或三孔检测（复孔），以获得更可靠的定量结果。

保存与运输

• 未开封试剂盒：避光保存于2 ℃‑8 ℃，有效期24个月。
• 启用后：请参照产品说明书分别存放各组分。
• 运输：全程使用蓝冰冷链包装，确保试剂活性。

安全警示 & 免责声明

本产品仅限科研用途，不用于人类或临床诊断。使用本套件时请遵循所在机构的安全规程。对因专利侵权或其他违规使用导致的后果，供应方不承担任何责任。

产品背景概述

脂联素（Adiponectin，亦称Acrp30）是由脂肪组织及胎盘分泌的蛋白质激素，参与葡萄糖调节、脂肪酸氧化及抗炎等多种代谢过程。其在血浆中的浓度相对较高，且可形成三聚体、六聚体及更高阶寡聚体。临床与动物模型研究表明，脂联素水平与2型糖尿病、肥胖、动脉粥样硬化及非酒精性脂肪肝等代谢疾病密切相关。通过本ELISA试剂盒定量小鼠血清或细胞培养上清中的脂联素，可为疾病模型的代谢状态评估提供重要参考。

规格与价格

规格	单价（人民币）	交货期
48 T	1,598.0	请咨询
96 T	2,558.0	请咨询

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Adiponectin ELISA Quantification Kit (Mouse Adiponectin ELISA Kit) – KTE3079-1

Product Overview

This kit is an EliKine™ core product, specifically designed for cellular analysis and ELISA applications. It employs a sandwich ELISA format for highly sensitive quantification of mouse adiponectin (Adiponectin). Suitable for cell culture supernatants, serum, and plasma samples, it enables researchers to obtain reliable data in studies of metabolism, inflammation, and cardiovascular diseases.

Key Technical Specifications

Parameter	Detail
Assay Type	ELISA (sandwich)
Species	Mouse
Detection Range	31.25 pg/mL – 2,000 pg/mL
Detection Sensitivity	31.25 pg/mL
Sample Types	Cell culture supernatant, serum, plasma
Catalog No.	KTE3079-1
Storage Conditions (unopened)	2 °C – 8 °C, protected from light, shelf life 24 months
Shipping Method	Ice packs (blue ice)

Kit Components

• Mouse Adiponectin Microplate
• Mouse Adiponectin Standard (lyophilized)
• Assay Buffer (10×)
• Mouse Adiponectin Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Features & Benefits

• Ultra‑high sensitivity (31.25 pg/mL) and a wide detection range.
• Highly specific antibodies exhibit minimal cross‑reactivity with adiponectin homologs, resulting in low background noise.
• Utilizes a proprietary streptavidin‑HRP complex to enhance signal intensity.
• Compatible with single, duplicate, or triplicate assays to improve experimental reproducibility.

Usage Precautions

• Reagents must be used only within the same batch; mixing different lot numbers or expired reagents is prohibited.
• Equilibrate at room temperature (approximately 20‑25 °C) for 30 minutes before use.
• Use disposable pipette tips to avoid cross‑contamination.
• Unused plate strips should be promptly returned to their packaging and stored sealed at 4 °C.
• Gently shake the plate every 10 minutes (low‑speed orbital shaker recommended) to ensure uniform reaction.
• Perform samples and standards in duplicate or triplicate wells for more reliable quantification.

Storage & Shipping

• Unopened kits: store protected from light at 2 °C‑8 °C, shelf life 24 months.
• After opening: refer to the product manual for storage conditions of individual components.
• Shipping: transport in blue‑ice cold chain packaging to preserve reagent activity.

Safety Warning & Disclaimer

This product is for research use only and is not intended for human or clinical diagnostics. Users must follow institutional safety procedures when handling this kit. The supplier assumes no liability for any consequences arising from patent infringement or other unauthorized use.

Product Background Overview

Adiponectin (also known as Acrp30) is a protein hormone secreted by adipose tissue and placenta, involved in glucose regulation, fatty‑acid oxidation, and anti‑inflammatory processes. It circulates in plasma at relatively high concentrations and can exist as trimers, hexamers, and higher‑order oligomers. Clinical and animal model studies have demonstrated strong associations between adiponectin levels and metabolic disorders such as type 2 diabetes, obesity, atherosclerosis, and non‑alcoholic fatty liver disease. Quantification of adiponectin in mouse serum or cell culture supernatant using this ELISA kit provides critical insight for evaluating the metabolic status of disease models.

Specifications & Pricing

Specification	Unit Price (CNY)	Lead Time
48 T	1,598.0	Please inquire
96 T	2,558.0	Please inquire

Note: The above information may be subject to change due to product improvements or updates; please contact Yakeyin Co. for the most current details.