NGFB/ Beta-NGF/ HSAN5/ NGF-B E

产品概览

中文名称：NGFB/ Beta-NGF/ HSAN5/ NGF-B ELISA 试剂盒（小鼠）
英文名称：Mouse NGF ELISA Kit (One Step Method)
货号：KTE4031-2
产品分类：核心产品 → 细胞分析 → ELISA

适用范围

本试剂盒专为 小鼠神经生长因子（NGF） 的定量检测设计，适用于蛋白类研究中的 ELISA 应用，尤其在以下样本类型中表现可靠：

• 血清
• 血浆
• 组织匀浆
• 细胞上清液
• 其他相关液体样本

检测原理

采用双抗体夹心技术（双抗体夹心法），关键步骤如下：

1. 采用特异性抗小鼠 NGF 抗体预包被酶标板孔。
2. 加入样本或标准品，使 NGF 与捕获抗体结合。
3. 加入生物素化检测抗体形成抗原-检测抗体复合物。
4. 加入链霉亲和素‑HRP，使酶标记定位于抗体复合物。
5. 洗涤除去未结合组分后，加入 HRP 底物（TMB），产生蓝色显色。
6. 加入终止液后显黄色，在 450 nm 读取 OD 值，OD 与 NGF 浓度呈正比。

试剂盒组成

• Mouse NGF Microplate
• Mouse NGF Standard (48 ng/mL)
• Standard Diluent
• Mouse NGF Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A
• HRP Substrate B
• Stop Solution
• Wash Buffer
• Plate Covers

检测范围与灵敏度

检测范围	0.3 ng/mL – 24 ng/mL
灵敏度（LOD）	0.06 ng/mL

特点 & 优势

• 极高的灵敏度与特异性，适用于低丰度 NGF 检测。
• 经验证对 NGF 类似物无显著交叉反应，背景噪声低。
• 一次性“一步法”操作流程，降低操作误差。
• 提供完整的配套试剂，完成全流程无需额外试剂。

操作要点

• 不同批号或超过有效期的试剂请勿混用。
• 使用前在室温放置 30 分钟，使试剂平衡。
• 建议使用一次性吸头，防止交叉污染。
• 未使用的板条请立即放回包装，密闭后置于 4 ℃保存。
• 充分混匀对结果影响大，推荐低速振荡或每 10 分钟手动摇匀。
• 样品和标准品建议设双孔或三孔复孔以提高数据可靠性。

保存与运输

保存建议：未开封的试剂盒请避光保存于 2‑8 ℃，保质期 6 个月。打开后请依据说明书分别保存各组分。

运输条件：使用蓝色冰袋（冷链）运输，确保全程低温。

法规声明 & 警告

本产品仅供研究使用，不适用于人类或临床诊断。使用本试剂盒进行的任何专利或许可证相关操作，风险由用户自行承担。

规格与采购信息

规格	单价（人民币）	交货期
48 T	1998.00	期货（3 天内发货）
96 T	3198.00	期货（3 天内发货）

关键字与检索信息

NGFB、Beta‑NGF、HSAN5、NGF‑B、Beta‑Nerve Growth Factor、Mouse NGF、ELISA、双抗体夹心法、神经生长因子、蛋白定量。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Chinese Name: NGFB/ Beta-NGF/ HSAN5/ NGF-B ELISA Kit (Mouse)
English Name: Mouse NGF ELISA Kit (One Step Method)
Catalog No.: KTE4031-2
Product Category: Core Products → Cell Analysis → ELISA

Applicable Scope

This kit is specifically designed for the quantitative detection of mouse nerve growth factor (NGF), suitable for ELISA applications in protein research, especially reliable in the following sample types:

• Serum
• Plasma
• Tissue homogenate
• Cell culture supernatant
• Other related liquid samples

Assay Principle

Utilizing a sandwich immunoassay (double‑antibody sandwich method), the key steps are as follows:

1. Coat microplate wells with a specific anti‑mouse NGF antibody.
2. Add samples or standards to allow NGF to bind the capture antibody.
3. Add biotinylated detection antibody to form antigen–detection antibody complex.
4. Add streptavidin‑HRP, which localizes the enzyme label to the antibody complex.
5. Wash to remove unbound components, then add HRP substrate (TMB) to generate a blue color.
6. Add stop solution to turn the color yellow; read absorbance at 450 nm, where OD is proportional to NGF concentration.

Kit Components

• Mouse NGF Microplate
• Mouse NGF Standard (48 ng/mL)
• Standard Diluent
• Mouse NGF Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A
• HRP Substrate B
• Stop Solution
• Wash Buffer
• Plate Covers

Detection Range and Sensitivity

Detection Range	0.3 ng/mL – 24 ng/mL
Sensitivity (LOD)	0.06 ng/mL

Features & Benefits

• Exceptionally high sensitivity and specificity, suitable for low‑abundance NGF detection.
• Validated to have no significant cross‑reactivity with NGF analogs, low background noise.
• One‑step workflow reduces procedural errors.
• Complete reagent set provided; full assay completed without additional reagents.

Critical Operational Points

• Do not mix reagents from different lots or past their expiration date.
• Allow reagents to equilibrate at room temperature for 30 minutes before use.
• Use disposable pipette tips to avoid cross‑contamination.
• Return unused plate strips to their packaging promptly, seal, and store at 4 °C.
• Thorough mixing greatly impacts results; recommend gentle orbital shaking or manual inversion every 10 minutes.
• Set samples and standards in duplicate or triplicate wells to improve data reliability.

Storage and Shipping

Storage Recommendation: Unopened kits should be stored protected from light at 2‑8 °C, with a shelf life of 6 months. Once opened, store each component according to the manual.

Shipping Conditions: Ship using blue ice packs (cold chain) to ensure temperature control throughout transit.

Regulatory Statement & Warning

This product is for research use only and is not intended for human or clinical diagnostic applications. Any patent or license‑related activities performed with this kit are undertaken at the user's own risk.

Specifications and Purchasing Information

Specification	Unit Price (CNY)	Lead Time
48 T	1998.00	Spot (ship within 3 days)
96 T	3198.00	Spot (ship within 3 days)

Keywords and Retrieval Information

NGFB, Beta‑NGF, HSAN5, NGF‑B, Beta‑Nerve Growth Factor, Mouse NGF, ELISA, sandwich immunoassay, nerve growth factor, protein quantification.

Note: The above information may change due to product optimization or upgrades; for the most current details, contact Yakein Co.