Insulin/INS ELISA KIT 胰岛素ELISA

Mouse Insulin ELISA Kit (One Step Method) – Insulin/INS ELISA 试剂盒

产品概述

本套装为EliKine™系列核心产品，专为小鼠胰岛素（Insulin）的定量检测设计，采用双抗体夹心法实现单步操作。适用于血清、血浆、组织匀浆、细胞上清等多种液体样本，兼容标准96孔酶标板读取仪，满足细胞分析中心的ELISA需求。

关键特性 & 优势

• 高灵敏度：检测下限 0.3 mU/L，覆盖 1.5–120 mU/L 宽范围。
• 特异性强：对小鼠胰岛素的类似物未出现显著交叉反应，背景噪声低。
• 一键式工作流程：从样本加样到显色仅需一步混合，显著降低手工操作时间。
• 兼容性：适配常规酶标仪（450 nm），支持自动化洗板系统。
• 样本通用：血清、血浆、组织提取液、细胞培养上清均可直接检测。

检测范围与灵敏度

检测范围	1.5 mU/L – 120 mU/L
灵敏度 (LOD)	0.3 mU/L

试剂盒组成

每套完整的试剂盒包括：

• Mouse Insulin Microplate
• Mouse Insulin Standard (240 mU/L)
• Standard Diluent
• Mouse Insulin Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

使用注意事项

• 不同批号或过期的试剂不能混用。
• 使用前请在室温下平衡 30 分钟。
• 操作期间使用一次性吸头，防止交叉污染。
• 未使用的板条需立即放回原包装，密封后在 4 ℃保存。
• 充分混匀对结果至关重要，推荐低速摇床或每 10 分钟手动摇匀一次。
• 建议对样品和标准进行双孔或三孔复测，以提高数据可靠性。

保存与运输

• 未开封试剂盒：避光保存于 2–8 ℃，有效期 6 个月。
• 启用后各组分按说明书要求冷链或冷藏保存。
• 运输条件：使用蓝色冰袋进行冷链运输。

规格与采购信息

包装规格	价格（人民币）	交付周期
48 T	1,998.00	期货（3 天内发货）
96 T	3,198.00	期货（3 天内发货）

法律声明 & 适用范围

本产品仅限于科研用途，不适用于人类或临床诊断。使用本产品不应违背任何专利或许可证，若因使用产生的专利侵权或其他违规行为，我方不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Insulin ELISA Kit (One‑Step Method) – Insulin/INS ELISA Reagent Kit

Product Overview

This kit is a core product of the EliKine™ series, specifically designed for the quantitative detection of mouse insulin (Insulin). It employs a double‑antibody sandwich format to achieve a one‑step workflow. Suitable for serum, plasma, tissue homogenates, cell supernatants and other liquid samples, it is compatible with standard 96‑well plate readers, meeting the ELISA needs of cell‑analysis facilities.

Key Features & Advantages

• High Sensitivity: Lower limit of detection 0.3 mU/L, covering a broad range of 1.5–120 mU/L.
• Strong Specificity: No significant cross‑reactivity with mouse insulin analogues; low background noise.
• One‑Click Workflow: From sample addition to color development requires only a single mixing step, dramatically reducing manual handling time.
• Compatibility: Works with conventional plate readers (450 nm) and supports automated plate‑washing systems.
• Universal Sample Types: Serum, plasma, tissue extracts, and cell culture supernatants can be assayed directly.

Detection Range and Sensitivity

Detection Range	1.5 mU/L – 120 mU/L
Sensitivity (LOD)	0.3 mU/L

Kit Composition

Each complete kit includes:

• Mouse Insulin Microplate
• Mouse Insulin Standard (240 mU/L)
• Standard Diluent
• Mouse Insulin Detection Antibody
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

Precautions for Use

• Do not mix reagents from different batches or expired lots.
• Allow all components to equilibrate at room temperature for 30 minutes before use.
• Use disposable tips during the procedure to prevent cross‑contamination.
• Unused strips must be returned to their original packaging immediately, sealed, and stored at 4 °C.
• Thorough mixing is critical for accurate results; a low‑speed shaker or manual vortex every 10 minutes is recommended.
• Perform duplicate or triplicate measurements of samples and standards to enhance data reliability.

Storage and Shipping

• Unopened kits: store protected from light at 2–8 °C; shelf life 6 months.
• After opening, each component should be kept under the temperature conditions specified in the instructions (cold chain or refrigeration).
• Shipping: transport on a blue ice pack under cold‑chain conditions.

Specifications and Purchasing Information

Package Specification	Price (CNY)	Delivery Lead Time
48 T	1,998.00	Futures (shipped within 3 days)
96 T	3,198.00	Futures (shipped within 3 days)

Legal Disclaimer & Scope of Application

This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Use of this product must not infringe any patents or licenses; we assume no liability for patent infringement or other violations arising from its use.

Note: The information above is subject to change due to product optimization or upgrades. For the most current details, please contact Yakeyin Co., Ltd.