IL-21 ELISA KIT 白介素21ELISA试剂盒(

Mouse IL-21 ELISA Kit (小鼠白介素21ELISA试剂盒)

货号：KTE3043-2 | 产品类型：EliKine™ 双抗体夹心法ELISA试剂盒

产品概述

本试剂盒采用双抗体夹心ELISA原理，专为精准定量检测小鼠来源样本（细胞培养上清、血清、血浆）中的白介素21（IL-21）而设计。作为细胞分析核心产品，该试剂盒在科研应用中表现出卓越的重复性与线性关系，适用于免疫调节、肿瘤免疫及自身免疫性疾病等相关领域的研究。

核心特性与技术优势

• 高灵敏度：最低检测浓度达31.25 pg/mL，可捕捉低丰度IL-21的表达变化
• 优异特异性：经严格验证，与小鼠IL-21类似物无交叉反应，背景干扰低
• 宽检测范围：31.25 pg/mL – 2,000 pg/mL，满足不同浓度样本的检测需求
• 稳定性强：试剂盒组分经优化配制，批间差小，结果可靠

试剂盒组分

• Mouse IL-21 Microplate（预包被酶标板）
• Mouse IL-21 Standard（冻干标准品）
• Standard Diluent（标准品稀释液）
• Assay Buffer (10×)（检测缓冲液）
• Mouse IL-21 Detect Antibody (100×)（检测抗体）
• Streptavidin-HRP (100×)（链霉亲和素-HRP）
• HRP Substrate (TMB)（显色底物）
• Stop Solution（终止液）
• Wash Buffer (20×)（洗涤缓冲液）
• Plate Covers（板盖）

检测参数

反应种属	小鼠
样本类型	细胞培养上清、血清、血浆
	夹心法ELISA
检测指标	IL-21

保存与运输

未开封试剂盒避光保存于2-8℃，有效期24个月；开封后请参照说明书保存各组分。运输采用冰袋（蓝冰）条件，保障试剂活性。

注意事项（关键步骤提示）

• 请勿混用不同批号或过期试剂
• 使用前需室温平衡30分钟，保证试剂活性稳定
• 推荐使用复孔检测（双孔或三孔），提高数据准确性
• 充分混匀是关键，建议使用低速振荡器或定期手动摇匀
• 实验过程中请使用一次性吸头，避免交叉污染

产品规格与价格

规格	价格（元）	货期
48 T	1598.0	期货（5天内发货）
96 T	2558.0	期货（5天内发货）

背景信息

IL-21是一种多效性细胞因子，主要由活化的CD4+ T细胞、Th2、Th17及滤泡性T细胞分泌，在调节NK细胞、细胞毒性T细胞功能中发挥关键作用。其在肿瘤免疫、自身免疫疾病及炎症反应中具有重要病理与治疗意义。本试剂盒为深入研究IL-21在小鼠模型中的表达与功能提供了可靠工具。

警告：本品仅供科研使用，不适用于临床诊断或人体应用。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse IL-21 ELISA Kit

Catalog Number: KTE3043-2 | Product Type: EliKine™ Sandwich ELISA Kit

Product Overview

This kit employs a sandwich ELISA principle and is specifically designed for the accurate quantitative detection of Interleukin-21 (IL-21) in mouse-derived samples (cell culture supernatants, serum, plasma). As a core cell analysis product, this kit demonstrates excellent reproducibility and linearity in research applications, making it suitable for studies in immune regulation, cancer immunology, autoimmune diseases, and related fields.

Core Features and Technical Advantages

• High Sensitivity: Minimum detectable concentration reaches 31.25 pg/mL, enabling detection of low-abundance IL-21 expression changes.
• Excellent Specificity: Rigorously validated, showing no cross-reactivity with mouse IL-21 analogs and low background interference.
• Wide Detection Range: 31.25 pg/mL – 2,000 pg/mL, meeting the detection needs for samples of varying concentrations.
• Strong Stability: Kit components are optimally formulated with low inter-assay variation, ensuring reliable results.

Kit Components

• Mouse IL-21 Microplate (Pre-coated)
• Mouse IL-21 Standard (Lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse IL-21 Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Assay Parameters

Reaction Species	Mouse
Sample Types	Cell culture supernatants, serum, plasma
Detection Method	Sandwich ELISA
Target	IL-21

Storage and Shipping

The unopened kit should be stored protected from light at 2-8°C and is stable for 24 months. After opening, please refer to the instructions for storage of individual components. The kit is shipped under ice pack (blue ice) conditions to ensure reagent activity.

Precautions (Key Step Reminders)

• Do not mix reagents from different lot numbers or use expired reagents.
• Equilibrate all reagents to room temperature for 30 minutes before use to ensure stable activity.
• Replicate testing (duplicate or triplicate wells) is recommended to improve data accuracy.
• Thorough mixing is critical; use a low-speed plate shaker or periodic manual shaking is advised.
• Use disposable pipette tips throughout the procedure to avoid cross-contamination.

Specifications and Pricing

Size	Price (RMB)	Availability
48 T	1598.0	Stock (ships within 5 business days)
96 T	2558.0	Stock (ships within 5 business days)

Background Information

IL-21 is a pleiotropic cytokine primarily secreted by activated CD4+ T cells, Th2, Th17, and follicular T cells. It plays a key role in regulating the functions of NK cells and cytotoxic T cells. IL-21 holds significant pathological and therapeutic importance in cancer immunology, autoimmune diseases, and inflammatory responses. This kit provides a reliable tool for in-depth investigation of IL-21 expression and function in mouse models.

Warning: This product is for research use only. Not intended for clinical diagnosis or human use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact YaKeyin Company for specific details.