小鼠免疫球蛋白G1 ELISA定量试剂盒(IgG1)

小鼠免疫球蛋白G1 ELISA定量试剂盒 (IgG1) – KTE3022-1

产品概述

英文名称：Mouse IgG1 ELISA Kit
中文名称：小鼠免疫球蛋白G1 ELISA定量试剂盒(IgG1)
货号：KTE3022-1
检测类型：ELISA（双抗体夹心法）
适用反应种属：小鼠
样本类型：细胞培养上清、血清、血浆

核心技术与优势

• 采用EliKine™ 专利夹心技术，实现 3.13 ng/mL 的极低检测限。
• 检测范围宽广：3.13 ng/mL – 200 ng/mL，覆盖大多数研究需求。
• 高特异性抗体阵列，未观察到显著交叉反应或背景干扰。
• 双抗体体系保证了信号与目标浓度的线性关系，适用于定量分析。

试剂盒组成（每套）

组件	说明
Mouse IgG1 Microplate	预包被抗体的96孔酶标板
Mouse IgG1 Standard (lyophilized)	冻干标准品，提供浓度梯度
Assay Buffer (10×)	工作缓冲液，稀释至使用浓度
HRP‑conjugated Mouse IgG1 Detect Antibody (100×)	酶标二抗，需稀释后使用
HRP Substrate (TMB)	显色底物，HRP 酶催化呈蓝色
Stop Solution	终止反应，使颜色转为黄色
Wash Buffer (20×)	洗涤缓冲，去除未结合组分
Plate Covers	防尘盖，保持板面洁净

关键实验步骤（概览）

1. 将冻干标准品复溶于Assay Buffer，配制标准曲线。
2. 加入待测样本或标准品至预包被微孔板中，37 °C孵育30 min。
3. 加入HRP‑conjugated 检测抗体，继续孵育。
4. 使用Wash Buffer 进行三次洗涤，去除非特异结合。
5. 加入TMB显色液，待颜色充分发展后加入Stop Solution。
6. 使用酶标仪在 450 nm 测定OD值，依据标准曲线计算样本中IgG1浓度。

使用建议

• 每个样本建议设置双孔或三孔复测以提高可靠性。
• 实验前所有试剂在室温平衡30 分钟。
• 混匀步骤建议使用低速振荡器或每10 分钟手动轻摇。
• 使用一次性吸头，防止交叉污染。
• 未使用的酶标板请立即回装包装，密封后在4 °C保存。

存储与运输

未开封存储：避光保存于2‑8 °C，保质期24 个月。
启用后：请参照说明书中各组分的具体保存条件。
运输方式：冰袋（蓝冰）冷链运输。

警示声明

本产品仅限于科研使用，不用于人类或临床诊断。使用本产品不应违反任何专利或许可协议，若因使用产生的专利侵权或其他法律风险，我司不承担责任。

产品规格与采购信息

包装规格	单价（CNY）	交货期
48 T	1,598.00	期货（5天内发货）
96 T	2,558.00	期货（5天内发货）

技术背景

IgG是血浆B细胞产生的主要抗体，在体液免疫中承担关键防御功能。小鼠IgG1是常用于免疫学研究的亚型之一，其水平变化可作为炎症、自身免疫或疫苗应答的指示指标。EliKine™ 小鼠IgG1 ELISA 通过双抗体夹心法，实现对样本中IgG1的高灵敏度定量，为细胞分析、药物研发及基础免疫学研究提供可靠的数据支持。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Immunoglobulin G1 ELISA Quantitation Kit (IgG1) – KTE3022-1

Product Overview

English Name: Mouse IgG1 ELISA Kit
Chinese Name: Mouse Immunoglobulin G1 ELISA Quantitation Kit (IgG1)
Catalog Number: KTE3022-1
Assay Type: ELISA (Sandwich ELISA)
Reactivity: Mouse
Sample Type: Cell culture supernatant, Serum, Plasma

Core Technology & Advantages

• Utilizes EliKine™ patented sandwich technology, achieving an extremely low detection limit of 3.13 ng/mL.
• Broad detection range: 3.13 ng/mL – 200 ng/mL, covering most research requirements.
• High-specificity antibody array, with no significant cross-reactivity or background interference observed.
• The dual-antibody system ensures a linear relationship between signal and target concentration, suitable for quantitative analysis.

Kit Components (Per Set)

Component	Description
Mouse IgG1 Microplate	Pre-coated 96-well microplate
Mouse IgG1 Standard (lyophilized)	Lyophilized standard, provided for concentration gradient
Assay Buffer (10×)	Working buffer, dilute to the use concentration
HRP‑conjugated Mouse IgG1 Detect Antibody (100×)	HRP-conjugated detection antibody, requires dilution before use
HRP Substrate (TMB)	Chromogenic substrate, turns blue upon HRP enzyme catalysis
Stop Solution	Stops the reaction, turning the color yellow
Wash Buffer (20×)	Wash buffer, removes unbound components
Plate Covers	Dust covers, maintain plate cleanliness

Key Experimental Steps (Overview)

1. Reconstitute the lyophilized standard in Assay Buffer to prepare the standard curve.
2. Add test samples or standards to the pre-coated microplate wells and incubate at 37 °C for 30 min.
3. Add the HRP‑conjugated detection antibody and continue incubation.
4. Wash three times using Wash Buffer to remove non-specifically bound components.
5. Add TMB substrate solution. After sufficient color development, add the Stop Solution.
6. Measure the OD at 450 nm using a microplate reader and calculate the IgG1 concentration in samples based on the standard curve.

Usage Recommendations

• It is recommended to set up duplicate or triplicate wells for each sample to improve reliability.
• Allow all reagents to equilibrate to room temperature for 30 minutes before the experiment.
• For mixing steps, use a low-speed shaker or gently shake manually every 10 minutes.
• Use disposable pipette tips to prevent cross-contamination.
• Immediately return unused microplate strips to the pouch, seal, and store at 4 °C.

Storage & Shipping

Unopened Storage: Store protected from light at 2‑8 °C. Shelf life: 24 months.
After Opening: Please refer to the specific storage conditions for each component in the manual.
Shipping Method: Shipped with ice packs (blue ice) under cold chain conditions.

Warning Statement

This product is for research use only, not for human or clinical diagnostic use. Use of this product should not violate any patent or licensing agreements. The company assumes no liability for patent infringement or other legal risks arising from the use of this product.

Specifications & Ordering Information

Package Size	Unit Price (CNY)	Delivery Time
48 T	1,598.00	Stock (Ships within 5 days)
96 T	2,558.00	Stock (Ships within 5 days)

Technical Background

IgG is the primary antibody produced by plasma B cells and plays a key defensive role in humoral immunity. Mouse IgG1 is one of the commonly studied subtypes in immunological research, and its level changes can serve as an indicator for inflammation, autoimmunity, or vaccine response. The EliKine™ Mouse IgG1 ELISA utilizes a sandwich ELISA method to achieve high-sensitivity quantitation of IgG1 in samples, providing reliable data support for cellular analysis, drug development, and basic immunology research.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.