IL-6Rα ELISA KIT 白细胞介素6受体αELIS

Mouse IL-6Rα ELISA Kit (小鼠白细胞介素6受体αELISA试剂盒)

货号：KTE3123-2

产品分类：核心产品 - 细胞分析

产品类型：ELISA定量试剂盒

产品概述

本试剂盒是一款基于双抗体夹心法原理的高性能ELISA检测试剂盒，专门用于定量检测小鼠来源样本中的白细胞介素6受体α（IL-6Rα）蛋白。作为EliKine™系列的核心产品，该试剂盒经过优化设计，适用于细胞培养上清、血清及血浆等多种样本类型，为科研人员在免疫学、炎症机制及细胞信号转导等研究领域提供可靠的分析工具。

技术特点与优势

• 高灵敏度：最低检测浓度达125 pg/mL，可准确识别低丰度目标蛋白。
• 优异特异性：经严格验证，对目标蛋白类似物无明显交叉反应或背景干扰。
• 宽检测范围：125 pg/mL至8,000 pg/mL的线性范围，满足不同浓度样本的检测需求。
• 稳定可靠：试剂盒组分经过严格质控，批间差小，确保实验结果的可重复性。
• 操作便捷：优化的实验流程与即用型试剂，缩短实验准备时间，提升工作效率。

试剂盒组分

• Mouse IL-6Rα预包被微孔板
• Mouse IL-6Rα标准品（冻干）
• 标准品稀释液
• 10× Assay Buffer
• 100× Mouse IL-6Rα Detect Antibody（生物素化检测抗体）
• 100× Streptavidin-HRP（链霉亲和素-HRP）
• HRP底物（TMB）
• 终止液
• 20× Wash Buffer
• 板盖

实验参数

反应性	小鼠	检测方法	夹心法ELISA
样本类型	细胞培养上清、血清、血浆	检测指标	IL-6Rα
灵敏度	125 pg/mL	检测范围	125 - 8,000 pg/mL
规格选项	48 T（货期请咨询）	规格选项	96 T（期货，5天内发货）

背景信息

白细胞介素6受体（IL-6R）是由IL-6受体α亚基（IL-6Rα）和信号转导蛋白gp130组成的复合物。可溶性IL-6Rα（约50 kDa）通过膜受体蛋白水解产生，能在溶液中与IL-6结合，进而与膜gp130作用，增强IL-6活性。其在炎症、自身免疫等病理过程中的调控机制尚在研究中，该试剂盒为探索IL-6/IL-6Rα信号通路提供了有效的定量工具。

保存与运输

保存建议：未开封试剂盒避光保存于2-8℃，保质期24个月；启用后请参照说明书保存各组分。

运输条件：冰袋（蓝冰）运输，确保试剂活性。

注意事项

• 请勿混合不同批号或过期试剂使用。
• 使用前请室温平衡30分钟，并按标签说明储存各组分。
• 实验中使用一次性吸头，避免交叉污染。
• 未使用的板条需立即密封，4℃保存。
• 推荐使用低速振荡器或定期手动混匀，确保反应充分。
• 为提高准确性，建议对样品和标准品进行复孔检测。

警告：本品仅供科研使用，不适用于人类或临床诊断。使用者应确保应用不违反相关专利或许可规定，厂家不对因使用本品导致的专利侵权或其他违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse IL-6Rα ELISA Kit (Mouse Interleukin-6 Receptor α ELISA Kit)

Item No.:KTE3123-2

Product Category:Core Products - Cell Analysis

Product Type:ELISA Quantitative Kit

Product Overview

This kit is a high‑performance ELISA detection kit based on the sandwich immunoassay principle, specifically designed for quantitative measurement of interleukin‑6 receptor α (IL‑6Rα) protein in mouse‑derived samples. As a core product of the EliKine™ series, the kit has been optimized for use with a variety of sample types, including cell culture supernatants, serum, and plasma, providing researchers with a reliable analytical tool for studies in immunology, inflammatory mechanisms, and cellular signal transduction.

Technical Features and Advantages

• High Sensitivity: Minimum detection limit of 125 pg/mL, enabling accurate identification of low‑abundance target protein.
• Excellent Specificity: Rigorously validated to exhibit no significant cross‑reaction or background interference with related proteins.
• Wide Dynamic Range: Linear range from 125 pg/mL to 8,000 pg/mL, meeting detection needs across various concentrations.
• Stable and Reliable: Kit components undergo stringent quality control with minimal inter‑batch variation, ensuring reproducibility of results.
• User‑Friendly Operation: Optimized workflow and ready‑to‑use reagents reduce preparation time and increase efficiency.

Kit Components

• Mouse IL-6Rα pre‑coated microplate
• Mouse IL-6Rα standard (lyophilized)
• Standard diluent
• 10× Assay Buffer
• 100× Mouse IL-6Rα Detection Antibody (biotinylated detection antibody)
• 100× Streptavidin‑HRP (streptavidin‑HRP)
• HRP substrate (TMB)
• Stop solution
• 20× Wash Buffer
• Plate seal

Assay Parameters

Species Reactivity	Mouse	Detection Method	Sandwich ELISA
Sample Types	Cell culture supernatant, serum, plasma	Analyte	IL-6Rα
Sensitivity	125 pg/mL	Detection Range	125 - 8,000 pg/mL
Package Options	48T (lead time on inquiry)	Package Options	96T (future stock, shipped within 5 days)

Background

Interleukin‑6 receptor (IL‑6R) consists of the IL‑6Rα subunit and the signal‑transducing protein gp130. Soluble IL‑6Rα (≈50 kDa) is generated by proteolytic cleavage of the membrane receptor and can bind IL‑6 in solution, subsequently interacting with membrane‑bound gp130 to enhance IL‑6 activity. Its regulatory mechanisms in inflammation, autoimmunity and other pathological processes are still under investigation. This kit provides an effective quantitative tool for exploring the IL‑6/IL‑6Rα signaling pathway.

Storage and Shipping

Storage Recommendation: Unopened kits should be stored protected from light at 2–8 °C, with a shelf life of 24 months; after opening, follow the manual for storage of individual components.

Shipping Conditions: Transport on ice packs (blue ice) to maintain reagent activity.

Precautions

• Do not mix reagents from different lot numbers or use expired reagents.
• Allow to equilibrate to room temperature for 30 minutes before use, and store each component as indicated on the label.
• Use disposable pipette tips during the assay to avoid cross‑contamination.
• Seal any unused plates immediately and store at 4 °C.
• Recommend gentle shaking or periodic manual mixing to ensure complete reactions.
• To improve accuracy, perform duplicate wells for samples and standards.

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that use does not infringe any patents or licensing agreements; the manufacturer assumes no responsibility for patent infringement or other violations arising from use of this product.

Note: The above information may change due to product optimization or upgrades; please contact Yakein Co. for the most current details.