小鼠免疫球蛋白A ELISA定量试剂盒(IgA)

产品概览

英文名称：Mouse IgA ELISA Kit
中文名称：小鼠免疫球蛋白A ELISA定量试剂盒 (IgA)
货号：KTE3020-1
产品分类：核心产品 → 细胞分析 → ELISA

适用范围

本套件专为小鼠免疫球蛋白A (IgA)的定量检测而设计，适用于以下样本类型：

• 细胞培养上清
• 血清
• 血浆

典型应用场景包括粘膜免疫研究、抗体分泌分析以及IgA相关的功能性实验。

检测技术

采用双抗体夹心ELISA（夹心法）实现高灵敏度定量。检测流程概述：

1. 板孔预包被抗小鼠IgA捕获抗体。
2. 加入样品或标准品，小鼠IgA与捕获抗体形成复合物。
3. 洗涤除去未结合组分。
4. 加入HRP标记的检测抗体，形成“捕获‑抗原‑检测”三元复合物。
5. 加入TMB底物，HRP催化产生蓝色显色，终止液使颜色转为黄色。
6. 在450 nm波长读取OD值，依据标准曲线计算样品中的IgA浓度。

关键性能指标

• 检测范围：0.39 ng/mL – 25 ng/mL
• 灵敏度（最低检测限）：0.39 ng/mL

产品组成

• Mouse IgA Microplate
• Mouse IgA Standard (lyophilized)
• Assay Buffer (10×)
• HRP‑conjugated Mouse IgA Detect Antibody (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

使用注意事项

• 不同批号或过期的试剂不能混用。
• 使用前请将试剂在室温平衡30 分钟。
• 实验过程中建议使用一次性吸头，防止交叉污染。
• 未使用的板条需放回包装、密闭保存，推荐于4 ℃短期保存。
• 充分混匀对结果至关重要，推荐使用低速振荡或每10 分钟手动摇匀。
• 样品和标准品建议采用双孔或三孔复测以提高可靠性。

存储与运输

未开封试剂盒：避光保存于2‑8 ℃，保质期24 个月。
开启后：请参照说明书对各组分进行分装或冷藏保存。
运输条件：使用蓝色冰袋（冰袋运输）保持低温。

警示声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本套件进行的任何研究均应遵守相关专利、许可证及伦理规定。本公司对因使用本产品可能产生的专利侵权或其他违规行为不承担责任。

背景信息

免疫球蛋白A（IgA）是粘膜免疫的关键抗体，主要以分泌型IgA（sIgA）二聚体形式存在于呼吸道、肠道等黏膜分泌物中。sIgA 能抵御蛋白水解酶的降解，维持在胃肠道等严苛环境中的稳定性，并抑制微生物的黏附与增殖。相较于IgG，IgA 对补体系统的活化较弱，但在抑制炎症与维持黏膜屏障方面发挥重要作用。

规格与报价

规格	单价（元）	货期
48 T	1598.0	期货（5 天内发货）
96 T	2558.0	期货（5 天内发货）

技术支持

如需帮助进行实验设计、标准曲线建立或结果解析，请联系您所在地区的销售代表或技术支持团队。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Mouse IgA ELISA Kit
Chinese Name: Mouse Immunoglobulin A ELISA Quantitative Kit (IgA)
Catalog Number: KTE3020-1
Product Category: Core Products → Cell Analysis → ELISA

Intended Use

This kit is specifically designed for the quantitative detection of Mouse Immunoglobulin A (IgA) in the following sample types:

• Cell Culture Supernatant
• Serum
• Plasma

Typical applications include mucosal immunity research, antibody secretion analysis, and IgA-related functional assays.

Assay Principle

Utilizes a Sandwich ELISA format for high-sensitivity quantitative detection. Assay procedure overview:

1. Wells are pre-coated with an anti-mouse IgA capture antibody.
2. Add samples or standards; mouse IgA binds to the capture antibody forming a complex.
3. Wash to remove unbound components.
4. Add HRP-conjugated detection antibody, forming a "capture-antibody–antigen–detection-antibody" ternary complex.
5. Add TMB substrate; HRP catalyzes a colorimetric reaction producing a blue color, which turns yellow upon addition of stop solution.
6. Measure the OD at 450 nm and calculate the IgA concentration in samples based on the standard curve.

Key Performance Parameters

• Detection Range: 0.39 ng/mL – 25 ng/mL
• Sensitivity (Minimum Detectable Concentration): 0.39 ng/mL

Kit Components

• Mouse IgA Microplate
• Mouse IgA Standard (lyophilized)
• Assay Buffer (10×)
• HRP-conjugated Mouse IgA Detection Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Precautions for Use

• Do not mix reagents from different lots or expired kits.
• Equilibrate all reagents to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the procedure to prevent cross-contamination.
• Return unused strips to the foil pouch, seal tightly, and store short-term at 4°C.
• Thorough mixing is critical; use low-speed orbital shaking or manual shaking every 10 minutes.
• It is recommended to assay samples and standards in duplicate or triplicate for reliability.

Storage and Shipping

Unopened Kit: Store protected from light at 2-8°C; stable for 24 months.
After Opening: Refer to the manual for aliquotting or refrigeration instructions for individual components.
Shipping Conditions: Shipped with blue ice packs to maintain a cool temperature.

Cautions

This product is for research use only. Not intended for human or clinical diagnostic use. Any research conducted using this kit must comply with relevant patents, licenses, and ethical regulations. The company assumes no responsibility for patent infringement or other violations that may arise from the use of this product.

Background

Immunoglobulin A (IgA) is a key antibody in mucosal immunity, primarily existing as a secretory IgA (sIgA) dimer in mucosal secretions such as those in the respiratory tract and gut. sIgA resists degradation by proteolytic enzymes, maintaining stability in harsh environments like the gastrointestinal tract, and inhibits microbial adhesion and proliferation. Compared to IgG, IgA has weaker complement system activation but plays a significant role in suppressing inflammation and maintaining the mucosal barrier.

Specifications and Pricing

Specification	Unit Price (RMB)	Lead Time
48 T	1598.0	Stock (Ships within 5 days)
96 T	2558.0	Stock (Ships within 5 days)

Technical Support

For assistance with experimental design, standard curve establishment, or result interpretation, please contact your local sales representative or technical support team.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.