血小板衍生生长因子BB酶联免疫试剂盒(小鼠) PDGF-BB

Mouse PDGF-BB ELISA Kit (One Step Method) - 小鼠血小板衍生生长因子BB酶联免疫试剂盒

货号: KTE4053-3 | 产品分类: 核心产品 | 产品中心: 细胞分析

产品概述

EliKine™ 小鼠血小板衍生生长因子BB (PDGF-BB) ELISA定量试剂盒是一款基于双抗体夹心法原理的高性能检测工具，专门设计用于精准定量小鼠血清、血浆、组织匀浆及细胞上清等液体样本中的PDGF-BB含量。该试剂盒凭借其极高的灵敏度（5 pg/mL）和宽广的检测范围（25 pg/mL - 2000 pg/mL），为血管生成、肿瘤微环境、骨骼代谢及组织修复等领域的科研工作提供了可靠的数据支持。

核心优势与特点

• 高灵敏度与特异性： EliKine™ 专利技术确保了对目标蛋白的精准识别，经严格验证，与类似物无明显交叉反应或背景干扰。
• 优化的“一步法”操作： 简化了传统ELISA流程，缩短了孵育时间，提升了实验效率，同时保证了结果的稳定性和可重复性。
• 即用型预制组分： 试剂盒内含预包被微孔板、标准品、检测抗体及全套缓冲液，开盒即用，减少了配制误差。
• 广泛的样本兼容性： 适用于血清、血浆、组织匀浆、细胞培养上清等多种生物样本类型。

技术参数

检测方法	双抗体夹心法ELISA
反应种属	小鼠
检测范围	25 pg/mL - 2000 pg/mL
灵敏度	5 pg/mL
样本类型	血清、血浆、组织匀浆、细胞上清及相关液体样本
孵育时间	优化后的“一步法”流程，总孵育时间显著缩短

试剂盒组分（详细列表）

• 预包被Mouse PDGF-BB微孔板 (48T或96T)
• Mouse PDGF-BB标准品 (4,000 pg/mL)
• 标准品稀释液
• Mouse PDGF-BB生物素化检测抗体
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP底物A液与B液 (TMB)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (Wash Buffer, 可按需稀释)
• 板盖 (Plate Covers)

注：所有组分均经过严格的质量控制，确保批次间稳定性。

背景信息

血小板衍生生长因子BB (PDGF-BB) 是PDGF家族中的重要成员，作为一种有效的促有丝分裂原和趋化因子，在血管生成、创伤愈合、炎症反应及骨骼发育等生理和病理过程中扮演关键角色。特别是在肿瘤研究中，PDGF-BB信号通路与肿瘤血管生成和微环境调控密切相关。本试剂盒为深入研究PDGF-BB在小鼠模型中的功能提供了精准的定量工具。

保存与运输

保存建议： 未开封试剂盒请避光保存于2-8℃，有效期6个月。启用后，请参照说明书分别保存各组分。

运输条件： 全程采用蓝冰（冰袋）低温运输，确保试剂活性。

订购信息

规格	价格 (人民币)	货期
48 T	1,998.0	期货（预计3个工作日内发货）
96 T	3,198.0	期货（预计3个工作日内发货）

重要注意事项

• 请勿混合使用不同批号的试剂或过期试剂。
• 使用前，所有试剂应在室温平衡30分钟。
• 实验过程中请使用一次性吸头，避免交叉污染。
• 建议对样品和标准品进行双孔或三孔检测（复孔），以提高数据准确性。
• 充分混匀是保证结果准确的关键步骤，推荐使用低速振荡器。

警告： 本产品仅用于科学研究，不适用于人类或临床诊断。使用前请务必阅读完整说明书。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse PDGF-BB ELISA Kit (One Step Method)

Catalog Number: KTE4053-3 | Product Category: Core Product | Product Center: Cell Analysis

Product Overview

The EliKine™ Mouse Platelet-Derived Growth Factor-BB (PDGF-BB) ELISA Quantification Kit is a high-performance detection tool based on the dual-antibody sandwich assay principle, specifically designed for the precise quantification of PDGF-BB levels in liquid samples such as mouse serum, plasma, tissue homogenates, and cell culture supernatants. With its exceptional sensitivity (5 pg/mL) and broad detection range (25 pg/mL - 2000 pg/mL), this kit provides reliable data support for research in areas such as angiogenesis, tumor microenvironment, bone metabolism, and tissue repair.

Core Advantages and Features

• High Sensitivity and Specificity: EliKine™ proprietary technology ensures accurate recognition of the target protein. Rigorously validated, it shows no significant cross-reactivity or background interference with analogs.
• Optimized "One-Step" Procedure: Simplifies the traditional ELISA workflow, shortens incubation time, improves experimental efficiency, while ensuring result stability and reproducibility.
• Ready-to-Use Pre-formulated Components: The kit contains pre-coated microplates, standards, detection antibodies, and a complete set of buffers, ready for immediate use, reducing preparation errors.
• Broad Sample Compatibility: Suitable for various biological sample types including serum, plasma, tissue homogenates, and cell culture supernatants.

Technical Parameters

Assay Method	Dual-antibody sandwich ELISA
Reactivity	Mouse
Detection Range	25 pg/mL - 2000 pg/mL
Sensitivity	5 pg/mL
Sample Type	Serum, plasma, tissue homogenates, cell culture supernatants, and related liquid samples
Incubation Time	Significantly reduced total incubation time with the optimized "One-Step" procedure

Kit Components (Detailed List)

• Pre-coated Mouse PDGF-BB Microplate (48T or 96T)
• Mouse PDGF-BB Standard (4,000 pg/mL)
• Standard Diluent
• Mouse PDGF-BB Biotinylated Detection Antibody
• Streptavidin-HRP
• HRP Substrate Solution A & B (TMB)
• Stop Solution
• Wash Buffer (can be diluted as needed)
• Plate Covers

Note: All components undergo strict quality control to ensure batch-to-batch consistency.

Background Information

Platelet-Derived Growth Factor-BB (PDGF-BB) is a crucial member of the PDGF family. As a potent mitogen and chemotactic factor, it plays a key role in physiological and pathological processes such as angiogenesis, wound healing, inflammatory response, and skeletal development. Particularly in cancer research, the PDGF-BB signaling pathway is closely associated with tumor angiogenesis and microenvironment regulation. This kit provides a precise quantitative tool for in-depth study of PDGF-BB function in mouse models.

Storage and Shipping

Storage Recommendation: Store unopened kit protected from light at 2-8°C, valid for 6 months. After opening, store components separately as per the manual.

Shipping Conditions: Shipped with blue ice (ice packs) under low-temperature conditions throughout to ensure reagent activity.

Ordering Information

Specification	Price (RMB)	Lead Time
48 T	1,998.0	Stock-to-order (expected to ship within 3 business days)
96 T	3,198.0	Stock-to-order (expected to ship within 3 business days)

Important Notes

• Do not mix reagents from different lot numbers or expired reagents.
• Before use, allow all reagents to equilibrate to room temperature for 30 minutes.
• Use disposable pipette tips during the experiment to avoid cross-contamination.
• Duplicate or triplicate measurements (replicates) for samples and standards are recommended to improve data accuracy.
• Thorough mixing is a crucial step for accurate results; the use of a low-speed shaker is recommended.

Warning: For research use only. Not intended for human or clinical diagnostic use. Please read the complete manual before use.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yaken Company.