小鼠胸腺基质淋巴细胞生成素 ELISA定量试剂盒(TSLP)

产品概述

EliKine™ 小鼠胸腺基质淋巴细胞生成素(TSLP) ELISA定量试剂盒 (货号: KTE3035-1) 是一款基于经典双抗体夹心法原理开发的高性能检测试剂盒，专为精准定量小鼠血清、血浆或细胞培养上清液中的TSLP蛋白浓度而设计。本产品归类为核心产品，是细胞因子研究领域的理想工具。

核心特性与优势

• 高灵敏度：最低检测浓度可达31.25 pg/mL，能够有效检测低丰度样本。
• 优异特异性：经严格验证，与目标蛋白的类似物无明显交叉反应，背景干扰低，确保结果的准确性。
• 宽线性范围：检测范围为31.25 pg/mL - 2,000 pg/mL，满足不同浓度样本的检测需求。
• 操作便捷：试剂盒提供预包被板条和优化好的缓冲液体系，实验流程标准化，节省优化时间。
• 批次稳定性：严格的生产质量控制，确保不同批次间结果的可比性。

检测原理

本试剂盒采用双抗体夹心ELISA法。预包被在小鼠TSLP特异性抗体的微孔板中，依次加入标准品或样本、生物素标记的检测抗体。经过孵育和洗涤后，加入链霉亲和素-HRP结合物。再次洗涤后，加入TMB底物进行显色反应。颜色的深度与样本中小鼠TSLP的浓度成正比，通过在450 nm波长下测定吸光度值即可进行定量分析。

产品组分

• 小鼠TSLP微孔板
• 小鼠TSLP标准品（冻干）
• 标准品稀释液
• 检测缓冲液 (10×)
• 小鼠TSLP检测抗体 (100×)
• 链霉亲和素-HRP (100×)
• HRP底物 (TMB)
• 终止液
• 洗涤缓冲液 (20×)
• 板盖

应用与背景

胸腺基质淋巴细胞生成素(TSLP)是一种主要由上皮细胞和成纤维细胞产生的细胞因子，在免疫调节，特别是在哮喘、特应性皮炎、过敏反应等Th2型免疫相关疾病的研究中具有重要意义。它通过激活树突状细胞，影响T细胞的分化和功能。本试剂盒为深入研究TSLP在小鼠模型中的生物学作用提供了可靠的工具。

技术参数

反应性	小鼠
样本类型	细胞培养上清、血清、血浆
检测范围	31.25 - 2,000 pg/mL
灵敏度	31.25 pg/mL
保存条件	未开封试剂盒请避光保存于2-8℃，有效期24个月
运输条件	冰袋运输（蓝冰）

规格与价格

规格	价格 (人民币)	货期
48 T	1,598.0	请咨询
96 T	2,558.0	请咨询

重要注意事项

• 请勿将不同批次的试剂混合使用，或使用已过有效期的试剂。
• 所有试剂使用前请在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 为保证反应充分，建议在孵育过程中使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对标准品和样品进行复孔检测。

警告：本品仅供科研使用，不适用于人类或临床诊断。使用本产品应确保不侵犯任何第三方专利权或遵守相关许可证规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

EliKine™ Mouse Thymic Stromal Lymphopoietin (TSLP) ELISA Quantitation Kit (Catalog No.: KTE3035-1) is a high‑performance assay kit developed on the classic sandwich ELISA principle, specifically designed for precise quantification of TSLP protein concentrations in mouse serum, plasma, or cell‑culture supernatants. This product is classified as a core product and serves as an ideal tool in cytokine research.

Key Features and Advantages

• High Sensitivity: Lower limit of detection reaches 31.25 pg/mL, enabling reliable detection of low‑abundance samples.
• Excellent Specificity: Rigorously validated with no significant cross‑reactivity to homologous proteins, low background interference, ensuring accurate results.
• Broad Linear Range: Quantitation range from 31.25 pg/mL to 2,000 pg/mL, accommodating samples with varying concentrations.
• Ease of Use: Kit includes pre‑coated plates and optimized buffer system, standardizing workflow and saving assay development time.
• Batch Consistency: Stringent manufacturing quality control guarantees comparability of results across different lots.

Assay Principle

This kit employs a sandwich ELISA format. Mouse TSLP‑specific capture antibody is pre‑coated on microplate wells. Standards or samples are added, followed by a biotin‑labeled detection antibody. After incubation and washing, streptavidin‑HRP conjugate is introduced. Following a second wash, TMB substrate is added for color development. The intensity of the color is proportional to the concentration of mouse TSLP in the sample and is quantified by measuring absorbance at 450 nm.

Kit Components

• Mouse TSLP microplate
• Mouse TSLP standard (lyophilized)
• Standard diluent
• Detection buffer (10×)
• Mouse TSLP detection antibody (100×)
• Streptavidin‑HRP (100×)
• HRP substrate (TMB)
• Stop solution
• Wash buffer (20×)
• Plate seal

Applications and Background

Thymic stromal lymphopoietin (TSLP) is a cytokine predominantly produced by epithelial and fibroblast cells. It plays a pivotal role in immune regulation, especially in Th2‑type immune‑related diseases such as asthma, atopic dermatitis, and allergic responses. TSLP activates dendritic cells, influencing T‑cell differentiation and function. This kit provides a reliable tool for investigating the biological role of TSLP in mouse models.

Technical Specifications

Species Reactivity	Mouse
Sample Types	Cell culture supernatant, serum, plasma
Detection Range	31.25 – 2,000 pg/mL
Sensitivity	31.25 pg/mL
Storage Conditions	Unopened kits should be stored protected from light at 2–8 °C; shelf life 24 months
Shipping Conditions	Transport on ice packs (blue ice)

Specifications and Pricing

Format	Price (CNY)	Lead Time
48 T	1,598.0	Please inquire
96 T	2,558.0	Please inquire

Important Precautions

• Do not mix reagents from different lots or use reagents past their expiration date.
• Allow all reagents to equilibrate to room temperature (~30 min) before use.
• Use disposable pipette tips throughout the assay to avoid cross‑contamination.
• Unused plate strips should be returned immediately to their original packaging, sealed, and stored at 4 °C.
• To ensure sufficient reaction, use a low‑speed shaker or manually agitate periodically during incubations.
• For more reliable data, assay standards and samples in duplicate wells.

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Use of this product must not infringe any third‑party patents or violate applicable licensing agreements.

Note: The information above is subject to change due to product optimization or upgrades; please contact Yakein Company for the most current details.