小鼠白介素18 ELISA定量试剂盒(IL-18)

产品概览

英文名称：Mouse IL-18 ELISA Kit  |  中文名称：小鼠白介素18 ELISA定量试剂盒（IL-18）

货号：KTE3028-1  |  检测物：IL-18（小鼠）

适用范围

• 应用领域：蛋白类研究，尤其是炎症因子与细胞因子分析
• 一级应用：ELISA（酶联免疫吸附测定）
• 核心产品类别：细胞分析 – ELISA试剂盒
• 样本类型：细胞培养上清、血清、血浆

技术概述

本套件基于 EliKine™ 双抗体夹心法，使用特异性包被抗体捕获样品中的小鼠IL-18，随后加入HRP‑标记检测抗体形成免疫复合物，TMB底物显色后在 450 nm 读取 OD 值，实现 62.5 pg/mL–4,000 pg/mL 线性定量。

关键性能指标

检测范围	62.5 pg/mL – 4,000 pg/mL
灵敏度	62.5 pg/mL
特异性	对IL-18同系物无明显交叉反应；背景干扰低

试剂盒组成（每套）

• Mouse IL-18 Microplate
• Mouse IL-18 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• HRP‑conjugated Mouse IL-18 Detect Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

操作建议

• 避免将不同批号或过期的试剂混用。
• 使用前请将试剂在室温平衡 30 分钟。
• 实验过程建议使用一次性吸头，防止交叉污染。
• 使用后未用的板条需密封回包装，置于 4 ℃保存。
• 充分混匀对结果影响显著，推荐低速振荡或每 10 分钟手动摇匀一次。
• 样品和标准建议做双孔或三孔复孔，以提高数据可靠性。

存储与运输

• 未开封保存：避光、2–8 ℃，有效期 24 个月。
• 启用后：按说明书分别保存各组分。
• 运输条件：使用蓝冰冷链（冰袋）配送。

警示声明

本产品仅限科研用途，不适用于人类或临床诊断。使用本试剂盒不应侵犯任何专利或许可证，若产生相关法律责任，本公司不承担。

产品背景

白介素‑18（IL‑18），又称干扰素‑γ诱导因子，是 IL‑1 超家族成员，由巨噬细胞等多种细胞分泌。IL‑18 能刺激 NK 细胞和特定 T 细胞产生 IFN‑γ，进而放大炎症反应；亦可在神经元中提升 β‑淀粉样蛋白的生成，关联阿尔茨海默病病理。准确测定小鼠体内 IL‑18 水平，对炎症机制、免疫调节及药物干预研究具有重要价值。

规格与报价

规格	单价 (￥)	交货期
48 条	1,598.0	期货（5 天内发货）
96 条	2,558.0	期货（5 天内发货）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Mouse IL-18 ELISA Kit  |  Chinese Name: Mouse Interleukin-18 ELISA Quantitation Kit (IL-18)

Catalog Number: KTE3028-1  |  Detection Target: IL-18 (Mouse)

Scope of Application

• Application Field: Protein research, especially inflammatory factor and cytokine analysis
• Primary Application: ELISA (Enzyme-Linked Immunosorbent Assay)
• Core Product Category: Cell Analysis – ELISA Kits
• Sample Types: Cell culture supernatants, serum, plasma

Technical Overview

This kit utilizes the EliKine™ Double Antibody Sandwich Method. A specific coated antibody captures mouse IL-18 in samples, followed by the addition of an HRP‑conjugated detection antibody to form an immune complex. Color development is achieved using TMB substrate, and OD values are read at 450 nm, enabling linear quantification within the range of 62.5 pg/mL to 4,000 pg/mL.

Key Performance Indicators

Detection Range	62.5 pg/mL – 4,000 pg/mL
Sensitivity	62.5 pg/mL
Specificity	No significant cross-reactivity with IL-18 homologs; low background interference

Kit Components (Per Kit)

• Mouse IL-18 Microplate
• Mouse IL-18 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• HRP‑conjugated Mouse IL-18 Detect Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Operational Recommendations

• Avoid mixing reagents from different lots or using expired reagents.
• Allow reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the procedure to prevent cross-contamination.
• Seal unused strips back into their packaging and store at 4 ℃ after use.
• Thorough mixing significantly impacts results; low-speed shaking or manual shaking every 10 minutes is recommended.
• It is advisable to run samples and standards in duplicate or triplicate to improve data reliability.

Storage and Shipping

• Unopened Storage: Store protected from light at 2–8 ℃. Shelf life is 24 months.
• After Opening: Store individual components as instructed in the manual.
• Shipping Conditions: Shipped using blue ice cold chain (ice packs).

Warning Statement

This product is intended for research use only and is not suitable for human or clinical diagnostic use. Use of this kit should not infringe upon any patents or licenses. The company assumes no liability for any related legal responsibilities arising from such use.

Product Background

Interleukin‑18 (IL‑18), also known as interferon‑γ inducing factor, is a member of the IL‑1 superfamily and is secreted by various cells including macrophages. IL‑18 stimulates NK cells and specific T cells to produce IFN‑γ, thereby amplifying inflammatory responses; it can also increase β‑amyloid production in neurons, linking it to Alzheimer's disease pathology. Accurate measurement of IL‑18 levels in mice is crucial for research on inflammatory mechanisms, immune regulation, and drug intervention.

Specifications and Pricing

Specification	Unit Price (￥)	Delivery Time
48 tests	1,598.0	Future goods (Ships within 5 days)
96 tests	2,558.0	Future goods (Ships within 5 days)

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult YaKeyin Company for specific details.