小鼠肿瘤坏死因子相关凋亡诱导配体 ELISA定量试剂盒(TR

产品概览

英文名称：Mouse TRAIL/TNFSF10 ELISA Kit
中文名称：小鼠肿瘤坏死因子相关凋亡诱导配体 ELISA 定量试剂盒（TRAIL）
货号：KTE3034-1
检测类别：ELISA（夹心法）
应用领域：蛋白类 • ELISA 应用 • 细胞分析（核心产品）

适用范围

本试剂盒专为小鼠来源的样本设计，可用于细胞培养上清、血清及血浆中 TRAIL（TNFSF10）的定量检测，帮助研究凋亡信号通路、死亡受体交互以及 NF‑κB 相关的炎症调控。

关键特点 & 优势

• 高灵敏度：检测下限 15.63 pg/mL。
• 宽检测范围：15.63 pg/mL – 1 000 pg/mL，满足低至中等浓度的样本分析需求。
• 特异性强：采用双抗体夹心结构，未观察到显著交叉反应或背景干扰。
• EliKine™ 技术平台：高亲和力预包被捕获抗体，确保稳固的固相捕获。
• 多孔板规格可选：48 条或 96 条，适配不同实验规模。

试剂盒组成

• Mouse TRAIL Microplate（预包被高亲和力抗体）
• Mouse TRAIL Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse TRAIL Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

性能规格

检测范围	15.63 pg/mL – 1 000 pg/mL
灵敏度	15.63 pg/mL
样本类型	细胞培养上清、血清、血浆
检测方法	夹心 ELISA（双抗体）

使用指南要点

• 试剂仅限同一批号使用，勿混用不同批次或超过有效期的试剂。
• 使用前请将所有试剂在室温（≈25 °C）平衡 30 分钟。
• 实验过程中建议使用一次性吸头，防止交叉污染。
• 未使用的板条需立即放回包装并在 4 °C 密封保存。
• 混匀是关键：推荐低速振荡或每 10 分钟手动轻摇一次。
• 样品和标准建议设置双孔或三孔复孔，以提升数据可靠性。

保存与运输

• 未开封试剂盒：避光保存于 2‑8 °C，保质期 24 个月。
• 启用后：请依据说明书对各组分分别保存。
• 运输方式：使用蓝色冰袋冷链运输，确保全程温度受控。

安全与合规声明

本产品仅限科研用途，不适用于人类或临床诊断。使用本试剂盒进行的任何研究均应遵守相关专利、许可证及伦理规定。因使用本产品可能产生的专利侵权或其他违规行为，供货方不承担任何责任。

技术背景

TRAIL（又名 CD253，TNFSF10）是由多种正常组织细胞分泌的凋亡诱导配体。它通过结合死亡受体 DR4（TRAIL‑RI）和 DR5（TRAIL‑RII）激活 caspase‑8，进而触发下游 caspase‑3/6/7 级联，引发细胞凋亡。TRAIL 亦可与无胞浆区的 DcR1（中和受体）及含截短死亡结构域的 DcR2（可激活 NF‑κB）相互作用，在特定细胞环境中调节死亡与存活信号的平衡。
EliKine™ 小鼠 TRAIL ELISA 采用双抗体夹心酶联免疫吸附技术：预包被捕获抗体先固定于高亲和力酶标板；待测样本中的 TRAIL 与之结合后，加入生物素化检测抗体，再通过链霉亲和素‑HRP 复合物实现信号放大；最终以 TMB 底物显色，在 450 nm（参考 570‑630 nm）测定吸光度，浓度与光密度呈正比。

订购信息

规格	单价（人民币）	备注
48 条	1 598.0	请咨询具体交货时间
96 条	2 558.0	期货（5 天内发货）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Mouse TRAIL/TNFSF10 ELISA Kit
Chinese Name: Mouse Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand ELISA Quantitative Kit (TRAIL)
Catalog Number: KTE3034-1
Detection Category: ELISA (Sandwich Method)
Application Fields: Protein Category • ELISA Application • Cell Analysis (Core Product)

Scope of Application

This kit is specifically designed for samples derived from mice and can be used for the quantitative detection of TRAIL (TNFSF10) in cell culture supernatants, serum, and plasma, aiding in the study of apoptotic signaling pathways, death receptor interactions, and NF-κB-related inflammatory regulation.

Key Features & Advantages

• High Sensitivity: Lower detection limit of 15.63 pg/mL.
• Wide Detection Range: 15.63 pg/mL – 1,000 pg/mL, meeting the analysis needs for low to medium concentration samples.
• Strong Specificity: Utilizes a dual-antibody sandwich structure, with no significant cross-reactivity or background interference observed.
• EliKine™ Technology Platform: High-affinity pre-coated capture antibodies ensure stable solid-phase capture.
• Optional Microplate Specifications: 48 wells or 96 wells, adaptable to different experimental scales.

Kit Components

• Mouse TRAIL Microplate (Pre-coated with high-affinity antibody)
• Mouse TRAIL Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse TRAIL Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Performance Specifications

Detection Range	15.63 pg/mL – 1,000 pg/mL
Sensitivity	15.63 pg/mL
Sample Type	Cell Culture Supernatant, Serum, Plasma
Detection Method	Sandwich ELISA (Dual Antibody)

Key Usage Guidelines

• Use reagents only from the same lot number; do not mix reagents from different lots or those past their expiration date.
• Allow all reagents to equilibrate to room temperature (≈25 °C) for 30 minutes before use.
• Use disposable pipette tips during the experiment to prevent cross-contamination.
• Unused strips should be immediately returned to the pouch and stored sealed at 4 °C.
• Mixing is crucial: recommend low-speed shaking or gentle manual shaking every 10 minutes.
• It is advised to set up duplicate or triplicate wells for samples and standards to enhance data reliability.

Storage and Shipping

• Unopened kit: Store protected from light at 2-8 °C, shelf life 24 months.
• After opening: Store individual components separately according to the instructions.
• Shipping method: Shipped with blue ice packs under controlled temperature conditions.

Safety and Compliance Statement

This product is intended for research use only and is not suitable for human or clinical diagnosis. Any research conducted using this kit must comply with relevant patents, licenses, and ethical regulations. The supplier assumes no responsibility for any patent infringement or other violations that may arise from the use of this product.

Technical Background

TRAIL (also known as CD253, TNFSF10) is an apoptosis-inducing ligand secreted by various normal tissue cells. It activates caspase-8 by binding to death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII), subsequently triggering the downstream caspase-3/6/7 cascade and inducing apoptosis. TRAIL can also interact with DcR1 (a decoy receptor lacking a cytoplasmic domain) and DcR2 (which contains a truncated death domain and can activate NF-κB), modulating the balance between death and survival signals in specific cellular contexts.
The EliKine™ Mouse TRAIL ELISA employs a sandwich enzyme-linked immunosorbent assay technique: the pre-coated capture antibody is first immobilized on a high-affinity microplate; TRAIL from the sample binds to it, followed by the addition of a biotinylated detection antibody, and signal amplification is achieved via a streptavidin-HRP complex; finally, color development with TMB substrate is measured at 450 nm (reference 570-630 nm), with concentration being proportional to the optical density.

Ordering Information

Specification	Unit Price (RMB)	Remarks
48 wells	1,598.0	Please inquire for specific delivery time
96 wells	2,558.0	Future product (ships within 5 days)

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Arkmicro for specific details.