IL-TIF, Cytokine ZCYTO18, 重组蛋白

Human IL-22 Protein, His tag (Animal-Free) – 高纯度重组细胞因子

货号：PRP1901

产品概述

本品为重组人源白细胞介素-22（IL-22）蛋白，C端带有6×His标签，采用无动物源表达系统（E. coli）生产，具备高纯度（>98%）与极低内毒素水平（<0.1 EU/μg），适用于细胞培养及相关功能研究。

产品特性

• 表达系统：大肠杆菌（无动物源表达）
• 纯度：> 98%（SDS-PAGE 验证）
• 蛋白长度：147个氨基酸（Ala 33-Ile 179），预测分子量17.83 kDa
• 实际迁移分子量：约17 kDa（还原性SDS-PAGE）
• 标签：C端6×His标签
• 生物活性：经功能验证，可诱导A549细胞增殖，ED₅₀ < 0.5 ng/mL
• 内毒素：< 0.1 EU/μg（LAL法测定）

应用领域

• 细胞增殖与分化研究
• 先天免疫及炎症反应机制研究
• 抗菌肽及再生相关基因表达分析

使用说明

• 复溶建议：推荐使用配套复溶缓冲液，浓度不低于100 µg/mL
• 保存条件：冻干粉-20℃可保存1年；复溶后2-8℃可保存1周，稀释后分装-20℃/-80℃可保存3-6个月
• 注意事项：使用前请离心，避免反复冻融

背景信息

人IL-22（UniProt: Q9GZX6）是一种由Th17细胞、γδ T细胞及先天性淋巴细胞（ILCs）产生的α螺旋细胞因子，具有促进细胞存活、增殖及抗菌肽（如S100A、Reg3家族蛋白）合成的作用，在黏膜免疫与组织修复中发挥关键功能。

规格与价格

运输条件：冰袋运输（蓝冰）

注意：本品仅供科研使用，不适用于临床诊断或人体用途。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human IL-22 Protein, His tag (Animal-Free) – High-Purity Recombinant Cytokine

Catalog Number: PRP1901

Product Overview

This product is recombinant human Interleukin-22 (IL-22) protein with a C-terminal 6×His tag. It is produced using an animal component-free expression system (E. coli), featuring high purity (>98%) and an ultra-low endotoxin level (<0.1 EU/μg). It is suitable for cell culture and related functional studies.

Characteristics

• Expression System: E. coli (Animal Component-Free)
• Purity: > 98% (verified by SDS-PAGE)
• Protein Length: 147 amino acids (Ala 33-Ile 179), predicted molecular weight 17.83 kDa
• Apparent Molecular Weight: ~17 kDa (reducing SDS-PAGE)
• Tag: C-terminal 6×His tag
• Bioactivity: Functionally verified to induce A549 cell proliferation with an ED₅₀ < 0.5 ng/mL
• Endotoxin: < 0.1 EU/μg (determined by LAL assay)

Applications

• Research on cell proliferation and differentiation
• Study of innate immunity and inflammatory response mechanisms
• Analysis of antimicrobial peptide and regeneration-related gene expression

Usage Instructions

• Reconstitution Recommendation: Reconstitute with the provided buffer to a concentration not less than 100 µg/mL.
• Storage Conditions: Lyophilized powder is stable for 1 year at -20°C; after reconstitution, store at 2-8°C for 1 week. For diluted aliquots, store at -20°C/-80°C for 3-6 months.
• Note: Centrifuge the vial before opening and avoid repeated freeze-thaw cycles.

Background

Human IL-22 (UniProt: Q9GZX6) is an α-helical cytokine produced by Th17 cells, γδ T cells, and innate lymphoid cells (ILCs). It functions to promote cell survival, proliferation, and the synthesis of antimicrobial peptides (e.g., S100A, Reg3 family proteins), playing a key role in mucosal immunity and tissue repair.

Specifications & Pricing

Shipping Conditions: Shipped with ice packs (blue ice)

Note: This product is for research use only. Not intended for clinical diagnosis or human use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakyn for specific details.