CXCL2细胞因子,重组小鼠CXC趋化因子配体2,重组蛋白,

产品描述

Mouse CXCL2 Protein, His tag (Animal-Free)是一种高纯度重组蛋白，通过大肠杆菌表达系统生产，专为细胞生物学研究设计。该产品对应小鼠C-X-C基序趋化因子2（CXCL2），其氨基酸序列来源于小鼠CXCL2（Ala28-Asn100），并在N端带有His标签，便于纯化和检测。

产品特性

• 高纯度: 经SDS-PAGE检测，纯度超过98%，确保实验的一致性和可重复性。
• 低内毒素: 通过LAL法测定，每微克蛋白内毒素含量<0.1 EU，最大限度减少对细胞实验的干扰。
• 高生物活性: 通过其对转染人CXCR2的BaF3细胞的化学吸引能力进行测定，ED50 < 0.5 ng/mL，证实其具有优异的受体结合与信号激活能力。
• 无动物源成分（Animal-Free）: 在生产过程中避免使用任何动物源性成分，降低了引入外源病原体的风险。

产品应用

本品主要应用于细胞培养研究领域，特别是：

• 趋化性（Chemotaxis）实验
• 炎症反应机制研究
• 肿瘤微环境与免疫细胞迁移研究
• 信号通路（如casepas1激活）研究

使用说明

• 复溶: 开盖使用前请先离心。推荐使用无菌PBS (pH 7.4) 进行复溶，初始浓度不低于100 µg/mL。用户可根据后续实验需求，使用含载体蛋白（如0.1% BSA, 10% FBS, 5% HSA）的缓冲液进行进一步稀释。
• 保存: 冻干粉在-20°C下可稳定保存1年。复溶后于2-8°C可保存1周。稀释分装后，在-20°C或-80°C下可保存3-6个月，请避免反复冻融。

背景信息

C-X-C基序趋化因子2（CXCL2），也被称为生长调节癌基因β（GROβ），是CXC趋化因子家族的成员。它主要由巨噬细胞和单核细胞等免疫细胞表达，在介导中性粒细胞迁移、血管生成、炎症反应及肿瘤进展中扮演关键角色。其功能主要通过与其受体（如CXCR2）结合来介导。

规格与价格

运输与警告

运输条件: 本品采用蓝冰（冰袋）运输，以确保蛋白稳定性。

警告: 此产品仅用于科研研究，不适用于人类或临床诊断。使用本产品可能受限于某些专利许可，请用户自行负责确保其应用不侵犯任何专利权或其他权利。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Description

Mouse CXCL2 Protein, His tag (Animal-Free) is a high-purity recombinant protein produced using an E. coli expression system, specifically designed for cell biology research. This product corresponds to mouse C-X-C motif chemokine 2 (CXCL2). The amino acid sequence is derived from mouse CXCL2 (Ala28-Asn100) and features an N-terminal His tag to facilitate purification and detection.

Product Features

• High Purity: Purity exceeds 98% as determined by SDS-PAGE analysis, ensuring experimental consistency and reproducibility.
• Low Endotoxin: Endotoxin level is <0.1 EU per µg of protein as measured by the LAL method, minimizing interference in cell-based assays.
• High Bioactivity: The chemotactic activity on BaF3 cells transfected with human CXCR2 demonstrates an ED50 < 0.5 ng/mL, confirming its excellent receptor binding and signaling activation capabilities.
• Animal-Free: The production process avoids the use of any animal-derived components, reducing the risk of introducing adventitious pathogens.

Product Applications

This product is primarily used in cell culture research, particularly for:

• Chemotaxis assays
• Studies on inflammatory response mechanisms
• Research on tumor microenvironment and immune cell migration
• Investigations of signaling pathways (e.g., caspase-1 activation)

Usage Instructions

• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein using sterile PBS (pH 7.4) to a concentration not less than 100 µg/mL. For subsequent experimental needs, further dilution can be performed using buffers containing carrier proteins (e.g., 0.1% BSA, 10% FBS, 5% HSA).
• Storage: The lyophilized powder is stable for 1 year when stored at -20°C. After reconstitution, it can be stored at 2-8°C for up to 1 week. For aliquoted and diluted samples, storage at -20°C or -80°C is recommended for 3-6 months; avoid repeated freeze-thaw cycles.

Background Information

C-X-C motif chemokine 2 (CXCL2), also known as growth-regulated oncogene beta (GROβ), is a member of the CXC chemokine family. It is primarily expressed by immune cells such as macrophages and monocytes, and plays a key role in mediating neutrophil migration, angiogenesis, inflammatory responses, and tumor progression. Its functions are mainly mediated through binding to its receptor, such as CXCR2.

Specifications & Pricing

Shipping & Caution

Shipping Conditions: This product is shipped with blue ice (ice packs) to ensure protein stability.

Caution: This product is intended for research use only and is not suitable for human use or clinical diagnosis. The use of this product may be subject to certain patent licenses; users are responsible for ensuring their applications do not infringe upon any patent or other rights.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.