IFN-α2a细胞因子,重组人干扰素α2a,重组蛋白,E.c

Human IFN-α2a Protein (重组人干扰素α2a)

产品货号：PRP1037

产品规格与定价：

• 20 µg - ¥588 (现货，次日发货)
• 100 µg - ¥1,498 (现货，次日发货)
• 1 mg - ¥9,998 (货期请咨询)

产品概述

本品为高纯度、无标签的重组人干扰素α2a (Human IFN-α2a Protein)，通过E.coli表达系统重组表达，经严格质控验证，适用于细胞培养等前沿生物研究。

技术参数

• 基因来源：人源
• 基因ID：3440
• UniProt ID：P01563
• 表达宿主：大肠杆菌
• 蛋白长度：165个氨基酸
• 预测分子量：19.24 kDa
• 蛋白序列区域：Cys24-Glu188
• 标签：无标签

质量控制

• 纯度：经SDS-PAGE检测，纯度 > 99%
• 内毒素水平：采用LAL法检测，每1μg蛋白质中EU含量 < 1.0
• 活性测定 (ED₅₀)：使用TF-1细胞进行细胞毒性测定，其ED₅₀为0.8896 ng/mL

产品形式与复溶建议

本品为冻干粉形式，冻干缓冲液为无菌的150mM NaCl，20mM Tris，pH 8.0溶液。

复溶注意事项：开盖前请先离心。为获得最佳实验效果，推荐使用该产品配套的复溶缓冲液 (Reconstitution Buffer) 进行复溶，复溶浓度建议不低于100 µg/mL。复溶后可依据具体实验需求进行进一步稀释。

保存与运输

• 保存建议：冻干粉应在-20°C以下干燥环境保存。复溶后，蛋白溶液可在4°C下保存2-7天，或在-20°C下长期保存。为长期储存考虑，建议添加载体蛋白（如0.1% HSA或BSA）。请避免反复冻融。
• 运输条件：本品可常温运输。

背景信息

干扰素α (IFN-α) 是一个包含至少23种不同亚型的细胞因子家族。IFN-α2a是其中一种重要的亚型，由IFNA2基因编码。这些亚型的分子量通常在19-26 kDa之间，其氨基末端序列可变，但在115-151氨基酸位点之间存在一个高度保守的区域。本品重组表达了人IFN-α2a的功能蛋白片段。

使用警告

重要提示：本产品仅限于科研用途，不适用于人类、临床诊断或任何药物用途。用户应确保其应用不违反任何专利或许可证规定。对于因使用本产品而可能导致的专利侵权或其他违规行为，我方不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human IFN‑α2a Protein (Recombinant Human Interferon‑α2a)

Product Code: PRP1037

Product Specifications & Pricing:

• 20 µg – ¥588 (in stock, next‑day shipment)
• 100 µg – ¥1,498 (in stock, next‑day shipment)
• 1 mg – ¥9,998 (lead time: please inquire)

Product Overview

This is a high‑purity, tag‑free recombinant human interferon‑α2a (Human IFN‑α2a Protein) produced via an E. coli expression system. The product has undergone rigorous quality‑control verification and is suitable for cell‑culture and other cutting‑edge biomedical research.

Technical Parameters

• Gene Source: Human
• Gene ID: 3440
• UniProt ID: P01563
• Expression Host: Escherichia coli
• Protein Length: 165 amino acids
• Predicted Molecular Weight: 19.24 kDa
• Protein Sequence Region: Cys24‑Glu188
• Tag: None

Quality Control

• Purity: > 99 % as determined by SDS‑PAGE
• Endotoxin Level: Measured by LAL assay; < 1.0 EU per µg protein
• Activity Assay (ED₅₀): Determined by cytotoxicity assay in TF‑1 cells; ED₅₀ = 0.8896 ng/mL

Product Form & Reconstitution Recommendations

The product is supplied as a lyophilized powder; the lyophilization buffer consists of sterile 150 mM NaCl, 20 mM Tris, pH 8.0.

Reconstitution Guidance: Centrifuge before opening. For optimal experimental performance, use the accompanying Reconstitution Buffer. Reconstitute to a concentration of at least 100 µg/mL. After reconstitution, dilute further according to specific experimental requirements.

Storage & Shipping

• Storage Recommendations: Keep the lyophilized powder in a dry environment at ≤ ‑20 °C. After reconstitution, store the protein solution at 4 °C for 2–7 days or long‑term at ‑20 °C. For long‑term storage, consider adding a carrier protein (e.g., 0.1 % HSA or BSA). Avoid repeated freeze‑thaw cycles.
• Shipping Conditions: Can be shipped at ambient temperature.

Background Information

Interferon‑α (IFN‑α) comprises at least 23 distinct subtypes. IFN‑α2a is a key subtype encoded by the IFNA2 gene. These subtypes typically range from 19–26 kDa; the N‑terminal region varies, while a highly conserved segment exists between residues 115–151. This product expresses the functional fragment of human IFN‑α2a.

Usage Warning

Important Notice: This product is for research use only and is not intended for human, clinical diagnostic, or any therapeutic applications. Users must ensure that their use does not infringe any patents or license agreements. We assume no liability for any patent infringement or other violations arising from the use of this product.

Note: Information may change due to product optimization or upgrades. For the most current details, please contact Yakoin Co., Ltd.