CXCL5细胞因子,重组人CXC趋化因子配体5,重组蛋白,E

PRP1921：Animal-Free 重组人 CXCL5 (ENA-78) · His-tag

产品快照

• 英文名称：Human CXCL5 Protein, His tag (Animal-Free)
• 别名：ENA-78、Epithelial Neutrophil Activating Peptide-78
• 货号：PRP1921
• 表达宿主：大肠杆菌 E. coli
• 分子量：≈12 kDa
• 纯度：>98%（SDS-PAGE）
• 内毒素：<0.1 EU/µg（LAL 法）
• 生物活性：ED₅₀ <10 ng/mL（CXCR2-BaF3 趋化实验）
• 规格状态：5 µg、20 µg 现货；100 µg、1 mg 咨询

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序列与结构

本品覆盖人 CXCL5 成熟功能区 Arg45-Asn114，N 端自带 6×His 标签，便于一步 IMAC 纯化或后续检测。大肠杆菌表达体系无动物源性成分，降低外源病毒与朊病毒风险。

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复溶与保存

• 外观：无菌冻干粉，含 PBS (pH 7.4) 保护缓冲。
• 复溶：开盖前 12 000 × g 离心 30 s；建议用配套复溶缓冲液调至 ≥100 µg/mL，避免剧烈吹打。
• 稳定性：
  • 冻干粉：-20 °C 避干燥 12 个月；
  • 复溶原液：2-8 °C ≤1 周；
  • 稀释工作液（含 0.1% BSA 或 5% HSA）：-20 °C 或 -80 °C 3-6 个月，≤3 次冻融。

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应用方向

• 细胞培养：作为单一或联合趋化因子，定向诱导 CXCR2⁺ 中性粒细胞、内皮前体细胞迁移。
• 体外成管：与 VEGF-A、FGF-2 联用，加速人脐静脉内皮细胞（HUVEC）管腔形成模型。
• 炎症建模：激活 NF-κB 信号，用于研究 IL-1β/TNF-α 下游通路。
• 蛋白互作：His 标签可直接用于 SPR、BLI 或下拉实验，筛选小分子拮抗剂。

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规格与货期

规格	目录价 (CNY)	发货周期
5 µg	588	现货（次日发）
20 µg	1 498	现货（次日发）
100 µg	2 289	请咨询
1 mg	请咨询	请咨询

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运输与警告

冰袋运输（蓝冰）。

科研专用，不得用于人类诊断或治疗。 使用本产品请遵守当地法规，若涉及第三方专利，恕不承担相关责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

PRP1921: Animal‑Free Recombinant Human CXCL5 (ENA‑78) · His‑tag

Product Snapshot

• English Name: Human CXCL5 Protein, His tag (Animal‑Free)
• Synonyms: ENA‑78, Epithelial Neutrophil Activating Peptide‑78
• Catalog No.: PRP1921
• Expression Host: Escherichia coli E. coli
• Molecular Weight: ≈12 kDa
• Purity: >98% (SDS‑PAGE)
• Endotoxin: <0.1 EU/µg (LAL assay)
• Bioactivity: ED₅₀ <10 ng/mL (CXCR2‑BaF3 chemotaxis assay)
• Availability: 5 µg, 20 µg in stock; 100 µg, 1 mg on request

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Sequence and Structure

This product encompasses the mature functional region of human CXCL5 (Arg45‑Asn114). An N‑terminal 6×His tag is included for one‑step IMAC purification or downstream detection. The E. coli expression system is free of animal‑derived components, reducing the risk of exogenous viral and prion contaminants.

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Reconstitution and Storage

• Appearance: Sterile lyophilized powder, containing PBS (pH 7.4) stabilizing buffer.
• Reconstitution: Centrifuge at 12 000 × g for 30 s before opening; reconstitute with the supplied buffer to a concentration of ≥100 µg/mL, avoiding vigorous vortexing.
• Stability:
  • Lyophilized powder: Store at –20 °C, protected from moisture for up to 12 months;
  • Reconstituted stock: 2‑8 °C, ≤1 week;
  • Diluted working solution (containing 0.1 % BSA or 5 % HSA): Store at –20 °C or –80 °C for 3‑6 months, ≤3 freeze‑thaw cycles.

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Application Areas

• Cell culture: Used as a single or combinatorial chemokine to direct migration of CXCR2⁺ neutrophils and endothelial progenitor cells.
• In vitro tube formation: Combined with VEGF‑A and FGF‑2 to accelerate lumen formation in human umbilical vein endothelial cell (HUVEC) models.
• Inflammation modeling: Activates NF‑κB signaling for studies of downstream pathways of IL‑1β/TNF‑α.
• Protein interaction: The His tag enables direct use in SPR, BLI, or pull‑down assays for screening small‑molecule antagonists.

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Specifications and Lead Times

Specification	Catalog Price (CNY)	Delivery Lead Time
5 µg	588	In stock (next‑day shipment)
20 µg	1 498	In stock (next‑day shipment)
100 µg	2 289	Please inquire
1 mg	Please inquire	Please inquire

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Shipping and Warning

Shipped on blue dry ice packs.

For research use only; not for human diagnostic or therapeutic applications. Use of this product must comply with local regulations; we assume no responsibility for third‑party patent issues.

Note: The above information may change with product optimization or upgrades; for definitive details, please contact Akorn Biotech.