IFN-α1a细胞因子,重组小鼠干扰素-α1a,重组蛋白,E

重组小鼠IFN-α1a细胞因子（His标签，无动物源）

货号：PRP1161

英文全称：Mouse IFN alpha 1a Protein, His tag (Animal-Free)

产品概述

本产品为高纯度、高活性重组小鼠干扰素-α1a (IFN-α1a) 蛋白，采用无动物源的大肠杆菌表达系统制备，并融合N端6×His标签，便于纯化与检测。该细胞因子在先天免疫和抗病毒反应研究中具有关键作用，适用于细胞培养、信号转导研究及相关生物制药研发环节。

产品特点与优势

• 高纯度：经SDS-PAGE验证，纯度 > 98%，确保实验结果的可靠性。
• 动物源游离：生产过程中避免使用动物源性原料，降低外源因子污染风险。
• 低内毒素：每微克蛋白质中内毒素含量 < 0.1 EU（LAL法测定），适用于敏感细胞实验。
• 高比活性：比活性 > 4 × 10⁷ IU/mg，在抗EMC病毒感染的L929细胞模型中，ED₅₀ < 10 pg/mL。
• 现货供应：多规格可选，大部分规格可实现次日发货，助您加速科研进程。

技术参数

产品形式与保存

本品为经0.2 µm过滤后冻干的白色粉末，溶于1X PBS（pH 7.4）。建议复溶时使用推荐复溶缓冲液，浓度不低于100 µg/mL，复溶后可根据实验需求进一步稀释。

保存条件：冻干粉-20°C保存一年；复溶后2-8°C可保存一周；稀释后等分样品在含稳定剂（如0.1% BSA）的缓冲液中-20°C或-80°C可保存3-6个月，请避免反复冻融。

规格与价格

使用注意事项

• 使用前请离心处理冻干粉，确保完全溶解。
• 本品仅供科研使用，不适用于人体或临床诊断。
• 建议用户根据实际实验体系优化使用浓度与条件。

运输方式：蓝冰冰链运输，保障产品活性。

如需获取更多技术资料，包括蛋白序列详情，请访问UniProt链接：Q9Y275。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse IFN-α1a Cytokine (His Tag, Animal-Free)

Catalog Number: PRP1161

Full Name: Mouse IFN alpha 1a Protein, His tag (Animal-Free)

Product Overview

This product is a highly pure and highly active recombinant mouse interferon-alpha 1a (IFN-α1a) protein, produced using an animal-free E. coli expression system and fused with an N-terminal 6×His tag for simplified purification and detection. This cytokine plays a key role in innate immunity and antiviral response research, and is suitable for cell culture, signal transduction studies, and related biopharmaceutical R&D applications.

Features & Advantages

• High Purity: > 98% purity as verified by SDS-PAGE, ensuring reliable experimental results.
• Animal-Free: The production process avoids the use of animal-derived materials, reducing the risk of contamination by adventitious agents.
• Low Endotoxin: Endotoxin level < 0.1 EU per μg of protein (determined by LAL assay), suitable for sensitive cell-based assays.
• High Specific Activity: Specific activity > 4 × 10⁷ IU/mg; ED₅₀ < 10 pg/mL in an L929 cell model protected from EMC virus infection.
• Available from Stock: Multiple sizes available, with most sizes shipped the next day to accelerate your research.

Technical Specifications

Formulation & Storage

This product is supplied as a white lyophilized powder after filtration through a 0.2 µm filter, dissolved in 1X PBS (pH 7.4). It is recommended to reconstitute using the suggested reconstitution buffer to a concentration not less than 100 μg/mL. After reconstitution, it can be further diluted according to experimental needs.

Storage Conditions: Lyophilized powder can be stored at -20°C for one year. After reconstitution, store at 2-8°C for up to one week. Aliquot diluted samples in buffer containing a carrier protein (e.g., 0.1% BSA) and store at -20°C or -80°C for 3-6 months. Avoid repeated freeze-thaw cycles.

Size & Price

Precautions for Use

• Centrifuge the lyophilized powder briefly before use to ensure complete dissolution.
• This product is for research use only and is not intended for human or diagnostic use.
• Users are advised to optimize the concentration and conditions for their specific experimental system.

Shipping Method: Shipped on blue ice to ensure product activity.

For more technical data, including detailed protein sequence information, please visit the UniProt link: Q9Y275.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact AcroGene for specific details.