IFN-γ细胞因子,重组小鼠干扰素-γ,重组蛋白,E.col

重组小鼠干扰素-γ (IFN-γ) 蛋白，N端His标签

产品货号: PRP1015
产品名称: IFN-γ细胞因子,重组小鼠干扰素-γ,重组蛋白,E.coli,N-His
英文名称: Mouse IFN-γ protein, His Tag

产品概述

本品为高纯度、具有生物活性的重组小鼠干扰素-γ (IFN-γ) 蛋白。该蛋白通过大肠杆菌系统重组表达，并在N端融合了6×His标签，便于纯化和检测。干扰素-γ是一种由淋巴细胞产生的关键细胞因子，在免疫调节、抗病毒反应和细胞生长调控中扮演着核心角色。本产品经过严格的质量控制，适用于各类细胞培养及生物医学研究。

关键特性

• 高纯度: 经SDS-PAGE检测，纯度大于95%，确保实验结果的一致性和可靠性。
• 确切的生物活性: 通过功能性ELISA验证，其与小鼠IFNGR1结合的EC₅₀为144 ng/mL。在TF-1细胞毒性测定中，显示出的ED₅₀为0.03279 ng/mL，证明了其强大的功能活性。
• 低内毒素水平: 采用LAL法检测，每1 μg蛋白质中的内毒素(EU)含量小于1.0，最大限度减少对细胞实验的干扰。
• 明确的分子量: 由154个氨基酸组成，理论分子量为16.9 kDa，与实际SDS-PAGE结果一致。

产品详情

种属来源: 小鼠
表达宿主: 大肠杆菌
蛋白序列: 氨基酸序列来源于鼠源γ-IFN (UniProt ID: P01580, 片段His23-Cys155)，N端含有6xHis标签。
产品形式: 白色或微黄色冻干粉。
冻干缓冲液: 无菌的150mM NaCl, 20mM Tris, pH 8.0溶液。
蛋白长度: 重组蛋白片段

使用说明

复溶建议: 开盖前请先离心。建议使用提供的复溶缓冲液进行复溶，初始浓度不低于100 µg/mL，后续可根据具体实验需求进行稀释。
保存条件: 冻干粉应保存于-20℃以下干燥环境。复溶后，于4℃可稳定保存2-7天；长期保存请置于-20℃以下。为保持最佳稳定性，建议添加载体蛋白（如0.1% HSA或BSA）并分装保存，避免反复冻融。
运输条件: 常温运输。

背景信息

干扰素-γ (IFN-γ) 是II型干扰素，主要由活化的T淋巴细胞和自然杀伤(NK)细胞产生。它不仅是重要的抗病毒细胞因子，更是强大的免疫调节剂。IFN-γ能够激活巨噬细胞，增强抗原呈递，调节Th1/Th2平衡，并在细胞凋亡和肿瘤免疫监视中发挥关键作用。其在自身免疫性疾病、感染和癌症研究模型中具有广泛应用。

规格与价格

规格	价格 (人民币)	货期
20 μg	588	现货（次日发货）
100 μg	1498	现货（次日发货）
500 μg	4516	现货（次日发货）
1 mg	请咨询	请咨询

警告: 本品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。

别名: IFG; IFI; IFN gamma; Interferon Gamma; IFNG; 重组小鼠干扰素-gamma; IFN-γ蛋白

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interferon-γ (IFN-γ) Protein, N-terminal His Tag

Product Code: PRP1015
Product Name: IFN-γ cytokine, recombinant mouse interferon-γ, recombinant protein, E.coli, N-His
English Name: Mouse IFN-γ protein, His Tag

Product Overview

This product is a high‑purity, biologically active recombinant mouse interferon‑γ (IFN‑γ) protein. The protein is expressed in an Escherichia coli system and fused at the N‑terminus with a 6×His tag for facile purification and detection. Interferon‑γ is a key cytokine produced by lymphocytes that plays a central role in immune modulation, antiviral responses, and regulation of cell growth. The product undergoes stringent quality control and is suitable for a wide range of cell‑culture and biomedical research applications.

Key Features

• High purity: SDS‑PAGE analysis shows purity >95%, ensuring consistency and reliability of experimental results.
• Defined biological activity: Functional ELISA demonstrates an EC₅₀ of 144 ng/mL for binding to mouse IFNGR1. In TF‑1 cytotoxicity assays, an ED₅₀ of 0.03279 ng/mL confirms potent functional activity.
• Low endotoxin level: LAL assay indicates endotoxin (EU) < 1.0 per µg protein, minimizing interference in cell‑based assays.
• Accurate molecular weight: Composed of 154 amino acids with a theoretical mass of 16.9 kDa, consistent with observed SDS‑PAGE migration.

Product Details

Species origin: Mouse
Expression host: Escherichia coli
Protein sequence: Amino‑acid sequence derived from mouse γ‑IFN (UniProt ID: P01580, fragment His23‑Cys155) with an N‑terminal 6×His tag.
Product form: White or slightly yellow lyophilized powder.
Lyophilization buffer: Sterile solution containing 150 mM NaCl, 20 mM Tris, pH 8.0.
Protein length: Recombinant protein fragment

Usage Instructions

Reconstitution recommendation: Centrifuge before opening. Reconstitute with the supplied buffer to achieve an initial concentration ≥100 µg/mL; subsequent dilutions can be made according to experimental needs.
Storage conditions: Store lyophilized powder in a dry environment at ≤ ‑20 °C. After reconstitution, keep at 4 °C for 2–7 days; for long‑term storage, keep at ≤ ‑20 °C. For optimal stability, add a carrier protein (e.g., 0.1 % HSA or BSA) and aliquot to avoid repeated freeze‑thaw cycles.
Shipping conditions: Ambient temperature shipping.

Background Information

Interferon‑γ (IFN‑γ) is a type II interferon primarily produced by activated T lymphocytes and natural killer (NK) cells. It is not only a critical antiviral cytokine but also a potent immunomodulator. IFN‑γ activates macrophages, enhances antigen presentation, regulates the Th1/Th2 balance, and plays vital roles in apoptosis and tumor immune surveillance. It is widely used in models of autoimmune disease, infection, and cancer research.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
20 μg	588	In stock (next‑day shipment)
100 μg	1498	In stock (next‑day shipment)
500 μg	4516	In stock (next‑day shipment)
1 mg	Please inquire	Please inquire

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licensing agreements.

Synonyms: IFG; IFI; IFN gamma; Interferon Gamma; IFNG; recombinant mouse interferon‑gamma; IFN‑γ protein

Note: The above information may be updated due to product optimization or other factors; please contact Yakoin Inc. for the latest details.