- 询价
- Miltenyi Biotec
- 德国
- 130-124-884
- 2025年12月09日
- Intracellular flow cytometry
- human cell line
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Notch1
- 亚型：
recombinant human IgG1
- 形态：
Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
- 保存条件：
避光，2-8℃
- 克隆性：
REA357
- 标记物：
PE
- 适应物种：
human, mouse
- 保质期：
24个月
- 供应商：
Miltenyi Biotec
- 宿主：
human cell line
- 应用范围：
Intracellular flow cytometry
- 浓度：
1:50
- 抗体英文名：
Notch1 Antibody, anti-human/mouse, REAfinity™
- 抗体名：
Notch1 Antibody, anti-human/mouse, REAfinity™
- 规格：
100 tests in 200 µL
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
with deionized water, and mix Mouse osteocalcin standard, one vial, 200 ng, lyophilized: reconstitute standard with exactly 2.0 ml of sample buffer (yields a 100 ng/ml solution); use
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