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Notch1 Antibody, anti-human, P

E, REAfinity™, 100 tests in 200 µL
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  • 询价
  • Miltenyi Biotec已认证
  • 德国
  • 130-112-453
  • 2025年12月09日
  • Flow cytometry
  • human cell line
  • human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Notch1

    • 亚型

      recombinant human IgG1

    • 形态

      Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.

    • 保存条件

      避光,2-8℃

    • 克隆性

      REA849

    • 标记物

      PE

    • 适应物种

      human

    • 保质期

      24个月

    • 供应商

      Miltenyi Biotec

    • 宿主

      human cell line

    • 应用范围

      Flow cytometry

    • 浓度

      1:50

    • 抗体英文名

      Notch1 Antibody, anti-human, REAfinity™

    • 抗体名

      Notch1 Antibody, anti-human, REAfinity™

    • 规格

      100 tests in 200 µL

    Identification and enumeration of Notch1+ cells by flow cytometryClone REA849 recognizes the human neurogenic locus notch homolog protein 1 (Notch1) antigen, a 300 kDa single-pass type I membrane protein also known as translocation-associated notch protein (TAN-1). Following cleavage in the Golgi, notch forms a heterodimer composed of a 180–200 kDa disulfide-linked extracellular domain (Notch extracellular truncation (NEXT)), and a 120 kDa membrane-bound intracellular portion (Notch intracellular domain (NICD)). Upon ligand binding the latter is cleaved to form the notch intracellular domain transcription factor. Notch signaling controls cell fate decisions and is involved in multiple cellular differentiation steps during embryonic development and in adult organisms. In the immune system, Notch1 plays a non-redundant role for thymic T cell development. Notch1 is also expressed by peripheral T cells and upregulated upon T cell activation and also found on bone marrow hematopoietic stem cells, NK cells, B cells, and monocytes . | Additional information: Clone REA849 displays negligible binding to Fc receptors.

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    图标文献和实验
    该产品被引用文献
    Gray, G. E. et al. (1999) Human ligands of the Notch receptor. Am. J. Pathol. 154 (3): 785–794. | Weng, A. P. et al. (2004) Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia. Science 306 (5694): 269–271. | Arruga, F. et al. (2014) Functional impact of NOTCH1 mutations in chronic lymphocytic leukemia. Leukemia 28 (5): 1060–1070.
    相关实验
    • T-cell dependent antigen specific in situ staining (Pe)

      -conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining

    • AbC Anti-Mouse Bead Kit

      suspension. 5)        Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)        Add a pre-titrated

    • AbC Anti-Rat/Hamster Bead Kit

      1 μL   5)      Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC capture beads (Component A in the AbC Anti-Mouse Bead Kit) to each tube. 6)     

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    文献支持
    Notch1 Antibody, anti-human, PE, REAfinity™, 100 tests in 200 µL
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