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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
pFBOH-LIC
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号:
质粒名称: pFBOH-LIC
其他名称:
质粒大小（bp）: 6770
抗性: Ampicillin
质粒类型: Bacterial Expression
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平: high
蛋白标签: Hexahistidine tag with Thrombin cleavage
测序引物:
GenBank号:
其他属性: 5' sequencing primer: Polyhedrin forward, pFBOH-Fwd (CCGGATTATTCATACCGTCCCACCA) List of Sequencing Primers
Selectable markers: Gentamicin
质粒图谱:
质粒序列:
>Author sequence
gacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttg
ccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccg
tcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaa
cttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttgg
agtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattc
ttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaattt
aacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaa
cccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaat
gcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttttt
gcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagt
tgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccga
agaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgcc
gggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacag
aaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacac
tgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggg
gatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgaca
ccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttc
ccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccg
gctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactgg
ggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacg
aaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactca
tatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgata
atctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa
aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctacca
gcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgc
agataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcc
tacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccggg
ttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagc
ccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgct
tcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggag
cttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgat
ttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcct
ggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtatt
accgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgagg
aagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcagaccagc
cgcgtaacctggcaaaatcggttacggttgagtaataaatggatgccctgcgtaagcgggtgtgggcgga
caataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaactagacagaatagt
tgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaagcaaact
cttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggccaagggcatggtaaagacta
tattcgcggcgttgtgacaatttaccgaacaactccgcggccgggaagccgatctcggcttgaacgaatt
gttaggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagag
agccactgcgggatcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctca
tgcttgagcagattgatgagcgcggtggcaatgccctgcctccggtgctcgccggagactgcgagatcat
agatatagatctcactacgcggctgctcaaacctgggcagaacgtaagccgcgagagcgccaacaaccgc
ttcttggtcgaaggcagcaagcgcgatgaatgtcttactacggagcaagttcccgaggtaatcggagtcc
ggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaagatcaagagcagcccgcatgg
atttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccacctaactttgtttt
agggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaaca
tcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataa
caagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtg
agcgcatacgctacttgcattacagtttacgaaccgaacaggcttatgtcaactgggttcgtgccttcat
ccgtttccacggtgtgcgtcacccggcaaccttgggcagcagcgaagtcgaggcatttctgtcctggctg
gcgaacgagcgcaaggtttcggtctccacgcatcgtcaggcattggcggccttgctgttcttctacggca
aggtgctgtgcacggatctgccctggcttcaggagatcggaagacctcggccgtcgcggcgcttgccggt
ggtgctgaccccggatgaagtggttcgcatcctcggttttctggaaggcgagcatcgtttgttcgcccag
gactctagctatagttctagtggttggctacgtatactccggaatattaatagatcatggagataattaa
aatgataaccatctcgcaaataaataagtattttactgttttcgtaacagttttgtaataaaaaaaccta
taaatattccggattattcataccgtcccaccatcgggcgcggatctcggtccgaaaaccatgggcagca
gccatcatcatcatcatcacagcagcggcctggttccgcgtggtagtattatgagttctcctcctgaaag
atccataacttcgtatagcatacattatacgaagttatgcggccgcgacgtccacatatacctgccgttc
actattatttagtgaaatgagatattatgatattttctgaattgtgattaaaaaggcaactttatgccca
tgcaacagaaactataaaaaatacagagaatgaaaagaaacagatagattttttagttctttaggcccgt
agtctgcaaatccttttatgattttctatcaaacaaaagaggaaaatagaccagttgcaatccaaacgag
agtctaatagaatgaggtcgaaaagtaaatcgcgcgggtttgttactgataaagcaggcaagacctaaaa
tgtgtaaagggcaaagtgtatactttggcgtcaccccttacatattttaggtctttttttattgtgcgta
actaacttgccatcttcaaacaggagggctggaagaagcagaccgctaacacagtacataaaaaaggaga
catgaacgatgaacatcaaaaagtttgcaaaacaagcaacagtattaacctttactaccgcactgctggc
aggaggcgcaactcaagcgtttgcgaaagaaacgaaccaaaagccatataaggaaacatacggcatttcc
catattacacgccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaagttcctgagt
tcgattcgtccacaattaaaaatatctcttctgcaaaaggcctggacgtttgggacagctggccattaca
aaacgctgacggcactgtcgcaaactatcacggctaccacatcgtctttgcattagccggagatcctaaa
aatgcggatgacacatcgatttacatgttctatcaaaaagtcggcgaaacttctattgacagctggaaaa
acgctggccgcgtctttaaagacagcgacaaattcgatgcaaatgattctatcctaaaagaccaaacaca
agaatggtcaggttcagccacatttacatctgacggaaaaatccgtttattctacactgatttctccggt
aaacattacggcaaacaaacactgacaactgcacaagttaacgtatcagcatcagacagctctttgaaca
tcaacggtgtagaggattataaatcaatctttgacggtgacggaaaaacgtatcaaaatgtacagcagtt
catcgatgaaggcaactacagctcaggcgacaaccatacgctgagagatcctcactacgtagaagataaa
ggccacaaatacttagtatttgaagcaaacactggaactgaagatggctaccaaggcgaagaatctttat
ttaacaaagcatactatggcaaaagcacatcattcttccgtcaagaaagtcaaaaacttctgcaaagcga
taaaaaacgcacggctgagttagcaaacggcgctctcggtatgattgagctaaacgatgattacacactg
aaaaaagtgatgaaaccgctgattgcatctaacacagtaacagatgaaattgaacgcgcgaacgtcttta
aaatgaacggcaaatggtacctgttcactgactcccgcggatcaaaaatgacgattgacggcattacgtc
taacgatatttacatgcttggttatgtttctaattctttaactggcccatacaagccgctgaacaaaact
ggccttgtgttaaaaatggatcttgatcctaacgatgtaacctttacttactcacacttcgctgtacctc
aagcgaaaggaaacaatgtcgtgattacaagctatatgacaaacagaggattctacgcagacaaacaatc
aacgtttgcgcctagcttcctgctgaacatcaaaggcaagaaaacatctgttgtcaaagacagcatcctt
gaacaaggacaattaacagttaacaaataaaaacgcaaaagaaaatgccgatatcctattggcattgacg
tcaggtggcacttttcgaggagatcatgcacatgatgacgaagcttgtcgagaagtactagaggatcata
atcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctga
aacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaa
tagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatc
aatgtatcttatcatgtctggatctgatcactgatatcgcctaggagatccgaaccagataagtgaaatc
tagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctat
tttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtc
cgcccacagcggggcatttttcttcctgttatgtttttaatcaaacatcctgccaactccatgtgacaaa
ccgtcatcttcggctactttttctctgtcacagaatgaaaatttttctgtcatctcttcgttattaatgt
ttgtaattgactgaatatcaacgcttatttgcagcctgaatggcgaatgg
>26099 with polyhedrin-fwd
TTTCGTACAGTTTTGTAATAAAAAAACCTATAAATATTCCGGATTATTCATACCGTCCCACCATCGGGCG
CGGATCTCGGTCCGAAAACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTTCCGCG
TGGTAGTATTATGAGTTCTCCTCCTGAAAGATCCATAACTTCGTATAGCATACATTATACGAAGTTATGC
GGCCGCGACGTCCACATATACCTGCCGTTCACTATTATTTAGTGAAATGAGATATTATGATATTTTCTGA
ATTGTGATTAAAAAGGCAACTTTATGCCCATGCAACAGAAACTATAAAAAATACAGAGAATGAAAAGAAA
CAGATAGATTTTTTAGTTCTTTAGGCCCGTAGTCTGCAAATCCTTTTATGATTTTCTATCAAACAAAAGA
GGAAAATAGACCAGTTGCAATCCAAACGAGAGTCTAATAGAATGAGGTCGAAAAGTAAATCGCGCGGGTT
TGTTACTGATAAAGCAGGCAAGACCTAAAATGTGTAAAGGGCAAAGTGTATACTTTGGCGTCACCCCTTA
CATATTTTAGGTCTTTTTTTATTGTGCGTAACTAACTTGCCATCTTCAAACAGGAGGGCTGGAAGAAGCA
GACCGCTAACACAGTACATAAAAAAGGAGACATGAACGATGAACATCAAAAAGTTTGCAAAACAAGCAAC
AGTATTAACCTTTACTACCGCACTGCTGGCAGGAGGCGCAACTCAAGCGTTTGCGAAAGAAACGAACCAA
AAGCCATATAAGGAAACATACGGCATTTCCCATATTACACGCCATGATATGCTGCAAATCCCTGAACAGC
AAAAAAATGAAAA
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文献和实验The protocol for LIC by Exonuclease III
The protocol for LIC by Exonuclease III 梁耀极 1. Design the primers with 15-bp overlap; 2. Digest the vector by proper restriction enzyme; For getting high quality vectors, there are some good advices as follows: 1) The restriction
（ doisyno-lic acid）系物质，或雌烷的 D环变为六环的同型雌酮等。有关这些结构不同而作用相同的原因目前尚不清楚。雌激素的鉴定法有 Allen-Doisy试验和子宫重量法。由于试验方法和给予激素方法的差异，所得结果不一定相同。都是以 0.1微克纯雌酮所引起的动作用（阴道上皮角质化）作为一国际单位（ IU）。而对苯甲酸盐的制剂，则从雌二醇的苯甲酸盐 0.1微克作为标准。
High-Throughput Cloning for Proteomics Research
Ligation-independent cloning (LIC) is a simple, rapid, and efficient method for high-throughput cloning. In this system, linear plasmid vector and insert DNA are treated to generate complementary single-stranded overhangs that anneal
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