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- 2025年11月21日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-48b(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2815y
质粒名称: pET-48b(+)
其他名称: pET48b, pET 48b
质粒大小（bp）: 5605
抗性: Kanamycin
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: 高
蛋白标签: N-Trx, N-His,N-HRV 3C, C-S, C-Thrombin
5′ 测序引物: T7或者Trx-F
5′ 测序引物序列: T7: 5′-TAATACGACTCACTATAGGG-3′; Trx-F: 5′-TTCCTCGACGCTAACCTG-3′
3′ 测序引物: T7t
3′ 测序引物序列: T7t: 5′-TGCTAGTTATTGCTCAGCGG-3′
GenBank号:
其他属性: Same as pET47 but also has Nterm Trx Tag; contains HRV 3C Protease cleavage site for fusion tag removal at low temperatures; Cterm thrombin cleavage site.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 120 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| S15 | 212 | 168 | |
| 6xHIS | 359 | 342 | |
| T7_transl_en_RBS | 792 | 776 | |
| lacO | 837 | 810 | |
| T7_promoter | 855 | 837 | |
| tet (300 – 563) | 891 | 1154 | |
| pBRrevBam_primer | 962 | 943 | |
| lacI | 1237 | 2328 | |
| ROP | 2900 | 3091 | |
| pGEX_3_primer | 3107 | 3085 | |
| pBR322_origin | 4125 | 3506 | |
| KanR2 | 4231 | 5046 | |
| f1_origin | 5577 | 5271 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 767 | 141 | |
| ORF frame 3 | 345 | 1496 | |
| ORF frame 1 | 1369 | 2328 | |
| ORF frame 1 | 4231 | 5046 |
| Enzyme Name | Cut |
|---|---|
| PacI | 141 |
| XhoI | 147 |
| BstBI | 187 |
| SacI | 238 |
| NotI | 242 |
| EagI | 242 |
| HindIII | 249 |
| SalI | 255 |
| AatII | 266 |
| AscI | 270 |
| StuI | 280 |
| EcoRI | 289 |
| BamHI | 295 |
| KpnI | 306 |
| XmaI | 306 |
| SmaI | 308 |
| SacII | 338 |
| SpeI | 417 |
| MscI | 426 |
| NdeI | 766 |
| XbaI | 804 |
| BclI | 1610 |
| ApaI | 1807 |
| HpaI | 2102 |
| NruI | 4319 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgtttagcag | cctaggttaa | ttaagcctcg | 150 |
| 151 | agagcagcag | aagtagagct | gtccatgtgc | tggcgttcga | atttagcagc | 200 |
| 201 | agcggtttct | ttactaccgc | gtggcaccag | agcgagctct | gcggccgcaa | 250 |
| 251 | gcttgtcgac | ggacgtcggg | cgcgccaagg | cctgtacaga | attcggatcc | 300 |
| 301 | tggtacccgg | gtccctgaaa | gaggacttca | agagccgcgg | agtgatggtg | 350 |
| 351 | atggtgatga | ccagaaccac | tagatggagt | aggagtagga | ggattgttat | 400 |
| 401 | tagaaccgcc | accaccacta | gtatggccag | aaccagaacc | ggccaggtta | 450 |
| 451 | gcgtcgagga | actctttcaa | ctgaccttta | gacagtgcac | ccactttggt | 500 |
| 501 | tgccgccact | tcaccgtttt | tgaacagcag | cagagtcggg | ataccacgga | 550 |
| 551 | tgccatattt | cggcgcagtg | ccagggtttt | gatcgatgtt | cagttttgca | 600 |
| 601 | acggtcagtt | tgccctgata | ttcgtcagcg | atttcatcca | gaatcggggc | 650 |
| 651 | gatcattttg | cacggaccgc | accactctgc | ccagaaatcg | acgaggatcg | 700 |
| 701 | ccccgtccgc | tttgagtaca | tccgtgtcaa | aactgtcgtc | agtcaggtga | 750 |
| 751 | ataattttat | cgctcatatg | tatatctcct | tcttaaagtt | aaacaaaatt | 800 |
| 801 | atttctagag | gggaattgtt | atccgctcac | aattccccta | tagtgagtcg | 850 |
| 851 | tattaatttc | gcgggatcga | gatcgatctc | gatcctctac | gccggacgca | 900 |
| 901 | tcgtggccgg | catcaccggc | gccacaggtg | cggttgctgg | cgcctatatc | 950 |
| 951 | gccgacatca | ccgatgggga | agatcgggct | cgccacttcg | ggctcatgag | 1000 |
| 1001 | cgcttgtttc | ggcgtgggta | tggtggcagg | ccccgtggcc | gggggactgt | 1050 |
| 1051 | tgggcgccat | ctccttgcat | gcaccattcc | ttgcggcggc | ggtgctcaac | 1100 |
| 1101 | ggcctcaacc | tactactggg | ctgcttccta | atgcaggagt | cgcataaggg | 1150 |
| 1151 | agagcgtcga | gatcccggac | accatcgaat | ggcgcaaaac | ctttcgcggt | 1200 |
| 1201 | atggcatgat | agcgcccgga | agagagtcaa | ttcagggtgg | tgaatgtgaa | 1250 |
| 1251 | accagtaacg | ttatacgatg | tcgcagagta | tgccggtgtc | tcttatcaga | 1300 |
| 1301 | ccgtttcccg | cgtggtgaac | caggccagcc | acgtttctgc | gaaaacgcgg | 1350 |
| 1351 | gaaaaagtgg | aagcggcgat | ggcggagctg | aattacattc | ccaaccgcgt | 1400 |
| 1401 | ggcacaacaa | ctggcgggca | aacagtcgtt | gctgattggc | gttgccacct | 1450 |
| 1451 | ccagtctggc | cctgcacgcg | ccgtcgcaaa | ttgtcgcggc | gattaaatct | 1500 |
| 1501 | cgcgccgatc | aactgggtgc | cagcgtggtg | gtgtcgatgg | tagaacgaag | 1550 |
| 1551 | cggcgtcgaa | gcctgtaaag | cggcggtgca | caatcttctc | gcgcaacgcg | 1600 |
| 1601 | tcagtgggct | gatcattaac | tatccgctgg | atgaccagga | tgccattgct | 1650 |
| 1651 | gtggaagctg | cctgcactaa | tgttccggcg | ttatttcttg | atgtctctga | 1700 |
| 1701 | ccagacaccc | atcaacagta | ttattttctc | ccatgaagac | ggtacgcgac | 1750 |
| 1751 | tgggcgtgga | gcatctggtc | gcattgggtc | accagcaaat | cgcgctgtta | 1800 |
| 1801 | gcgggcccat | taagttctgt | ctcggcgcgt | ctgcgtctgg | ctggctggca | 1850 |
| 1851 | taaatatctc | actcgcaatc | aaattcagcc | gatagcggaa | cgggaaggcg | 1900 |
| 1901 | actggagtgc | catgtccggt | tttcaacaaa | ccatgcaaat | gctgaatgag | 1950 |
| 1951 | ggcatcgttc | ccactgcgat | gctggttgcc | aacgatcaga | tggcgctggg | 2000 |
| 2001 | cgcaatgcgc | gccattaccg | agtccgggct | gcgcgttggt | gcggacatct | 2050 |
| 2051 | cggtagtggg | atacgacgat | accgaagaca | gctcatgtta | tatcccgccg | 2100 |
| 2101 | ttaaccacca | tcaaacagga | ttttcgcctg | ctggggcaaa | ccagcgtgga | 2150 |
| 2151 | ccgcttgctg | caactctctc | agggccaggc | ggtgaagggc | aatcagctgt | 2200 |
| 2201 | tgcccgtctc | actggtgaaa | agaaaaacca | ccctggcgcc | caatacgcaa | 2250 |
| 2251 | accgcctctc | cccgcgcgtt | ggccgattca | ttaatgcagc | tggcacgaca | 2300 |
| 2301 | ggtttcccga | ctggaaagcg | ggcagtgagc | gcaacgcaat | taatgtaagt | 2350 |
| 2351 | tagctcactc | attaggcacc | gggatctcga | ccgatgccct | tgagagcctt | 2400 |
| 2401 | caacccagtc | agctccttcc | ggtgggcgcg | gggcatgact | agcatgatcg | 2450 |
| 2451 | tgctcctgtc | gttgaggacc | cggctaggct | ggcggggttg | ccttactggt | 2500 |
| 2501 | tagcagaatg | aatcaccgat | acgcgagcga | acgtgaagcg | actgctgctg | 2550 |
| 2551 | caaaacgtct | gcgacctgag | caacaacatg | aatggtcttc | ggtttccgtg | 2600 |
| 2601 | tttcgtaaag | tctggaaacg | cggaagtcag | cgccctgcac | cattatgttc | 2650 |
| 2651 | cggatctgca | tcgcaggatg | ctgctggcta | ccctgtggaa | cacctacatc | 2700 |
| 2701 | tgtattaacg | aagcgctggc | attgaccctg | agtgattttt | ctctggtccc | 2750 |
| 2751 | gccgcatcca | taccgccagt | tgtttaccct | cacaacgttc | cagtaaccgg | 2800 |
| 2801 | gcatgttcat | catcagtaac | ccgtatcgtg | agcatcctct | ctcgtttcat | 2850 |
| 2851 | cggtatcatt | acccccatga | acagaaatcc | cccttacacg | gaggcatcag | 2900 |
| 2901 | tgaccaaaca | ggaaaaaacc | gcccttaaca | tggcccgctt | tatcagaagc | 2950 |
| 2951 | cagacattaa | cgcttctgga | gaaactcaac | gagctggacg | cggatgaaca | 3000 |
| 3001 | ggcagacatc | tgtgaatcgc | ttcacgacca | cgctgatgag | ctttaccgca | 3050 |
| 3051 | gctgcctcgc | gcgtttcggt | gatgacggtg | aaaacctctg | acacatgcag | 3100 |
| 3101 | ctcccggaga | cggtcacagc | ttgtctgtaa | gcggatgccg | ggagcagaca | 3150 |
| 3151 | agcccgtcag | ggcgcgtcag | cgggtgttgg | cgggtgtcgg | ggcgcagcca | 3200 |
| 3201 | tgacccagtc | acgtagcgat | agcggagtgt | atactggctt | aactatgcgg | 3250 |
| 3251 | catcagagca | gattgtactg | agagtgcacc | atatatgcgg | tgtgaaatac | 3300 |
| 3301 | cgcacagatg | cgtaaggaga | aaataccgca | tcaggcgctc | ttccgcttcc | 3350 |
| 3351 | tcgctcactg | actcgctgcg | ctcggtcgtt | cggctgcggc | gagcggtatc | 3400 |
| 3401 | agctcactca | aaggcggtaa | tacggttatc | cacagaatca | ggggataacg | 3450 |
| 3451 | caggaaagaa | catgtgagca | aaaggccagc | aaaaggccag | gaaccgtaaa | 3500 |
| 3501 | aaggccgcgt | tgctggcgtt | tttccatagg | ctccgccccc | ctgacgagca | 3550 |
| 3551 | tcacaaaaat | cgacgctcaa | gtcagaggtg | gcgaaacccg | acaggactat | 3600 |
| 3601 | aaagatacca | ggcgtttccc | cctggaagct | ccctcgtgcg | ctctcctgtt | 3650 |
| 3651 | ccgaccctgc | cgcttaccgg | atacctgtcc | gcctttctcc | cttcgggaag | 3700 |
| 3701 | cgtggcgctt | tctcatagct | cacgctgtag | gtatctcagt | tcggtgtagg | 3750 |
| 3751 | tcgttcgctc | caagctgggc | tgtgtgcacg | aaccccccgt | tcagcccgac | 3800 |
| 3801 | cgctgcgcct | tatccggtaa | ctatcgtctt | gagtccaacc | cggtaagaca | 3850 |
| 3851 | cgacttatcg | ccactggcag | cagccactgg | taacaggatt | agcagagcga | 3900 |
| 3901 | ggtatgtagg | cggtgctaca | gagttcttga | agtggtggcc | taactacggc | 3950 |
| 3951 | tacactagaa | ggacagtatt | tggtatctgc | gctctgctga | agccagttac | 4000 |
| 4001 | cttcggaaaa | agagttggta | gctcttgatc | cggcaaacaa | accaccgctg | 4050 |
| 4051 | gtagcggtgg | tttttttgtt | tgcaagcagc | agattacgcg | cagaaaaaaa | 4100 |
| 4101 | ggatctcaag | aagatccttt | gatcttttct | acggggtctg | acgctcagtg | 4150 |
| 4151 | gaacgaaaac | tcacgttaag | ggattttggt | catgaacaat | aaaactgtct | 4200 |
| 4201 | gcttacataa | acagtaatac | aaggggtgtt | atgagccata | ttcaacggga | 4250 |
| 4251 | aacgtcttgc | tctaggccgc | gattaaattc | caacatggat | gctgatttat | 4300 |
| 4301 | atgggtataa | atgggctcgc | gataatgtcg | ggcaatcagg | tgcgacaatc | 4350 |
| 4351 | tatcgattgt | atgggaagcc | cgatgcgcca | gagttgtttc | tgaaacatgg | 4400 |
| 4401 | caaaggtagc | gttgccaatg | atgttacaga | tgagatggtc | agactaaact | 4450 |
| 4451 | ggctgacgga | atttatgcct | cttccgacca | tcaagcattt | tatccgtact | 4500 |
| 4501 | cctgatgatg | catggttact | caccactgcg | atccccggca | aaacagcatt | 4550 |
| 4551 | ccaggtatta | gaagaatatc | ctgattcagg | tgaaaatatt | gttgatgcgc | 4600 |
| 4601 | tggcagtgtt | cctgcgccgg | ttgcattcga | ttcctgtttg | taattgtcct | 4650 |
| 4651 | tttaacagtg | atcgcgtatt | tcgtctcgct | caggcgcaat | cacgaatgaa | 4700 |
| 4701 | taacggtttg | gttgatgcga | gtgattttga | tgacgagcgt | aatggctggc | 4750 |
| 4751 | ctgttgaaca | agtctggaaa | gaaatgcata | aacttttgcc | attctcaccg | 4800 |
| 4801 | gattcagtcg | tcactcatgg | tgatttctca | cttgataacc | ttatttttga | 4850 |
| 4851 | cgaggggaaa | ttaataggtt | gtattgatgt | tggacgagtc | ggaatcgcag | 4900 |
| 4901 | accgatacca | ggatcttgcc | atcctatgga | actgcctcgg | tgagttttct | 4950 |
| 4951 | ccttcattac | agaaacggct | ttttcaaaaa | tatggtattg | ataatcctga | 5000 |
| 5001 | tatgaataaa | ttgcagtttc | atttgatgct | cgatgagttt | ttctaagaat | 5050 |
| 5051 | taattcatga | gcggatacat | atttgaatgt | atttagaaaa | ataaacaaat | 5100 |
| 5101 | aggggttccg | cgcacatttc | cccgaaaagt | gccacctgaa | attgtaaacg | 5150 |
| 5151 | ttaatatttt | gttaaaattc | gcgttaaatt | tttgttaaat | cagctcattt | 5200 |
| 5201 | tttaaccaat | aggccgaaat | cggcaaaatc | ccttataaat | caaaagaata | 5250 |
| 5251 | gaccgagata | gggttgagtg | ttgttccagt | ttggaacaag | agtccactat | 5300 |
| 5301 | taaagaacgt | ggactccaac | gtcaaagggc | gaaaaaccgt | ctatcagggc | 5350 |
| 5351 | gatggcccac | tacgtgaacc | atcaccctaa | tcaagttttt | tggggtcgag | 5400 |
| 5401 | gtgccgtaaa | gcactaaatc | ggaaccctaa | agggagcccc | cgatttagag | 5450 |
| 5451 | cttgacgggg | aaagccggcg | aacgtggcga | gaaaggaagg | gaagaaagcg | 5500 |
| 5501 | aaaggagcgg | gcgctagggc | gctggcaagt | gtagcggtca | cgctgcgcgt | 5550 |
| 5551 | aaccaccaca | cccgccgcgc | ttaatgcgcc | gctacagggc | gcgtcccatt | 5600 |
| 5601 | cgcca | 5605 |
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pET-48b(+)
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