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- 2025年11月20日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pBP108
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号:
质粒名称: pBP108
其他名称:
质粒大小（bp）: 4900
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Constructed from pBP103 (ATCC 77087) by inserting a 0.3 kb Alu-containing sequence from BLUR8 into the BamHI site. pBP108 (ATCC 77088) and pBP109 (ATCC 77089) differ in the orientation of the Alu sequence. [1] Acentric chromosome fragmentation vector targeting Alu sequences. Contains promoters for in vitro RNA synthesis. [1] Any unique restriction site between the targeting sequence and the telomere-adjacent sequence can be used to linearize the plasmid before transformation. [1] The EcoRV and ClaI sites between the SP6 promoter and the telomere sequence can be used for recovery from deletion derivatives of YAC insert sequences adjacent to the fragmentation site. [1] Restriction digests of the clone give the following sizes (kb): BamHI–4.6, 0.3; EcoRI–4.9; PstI–3.4, 1.15, 0.35. (ATCC staff) The order of the major features in this plasmid is: bla – HIS3 – lacZ'/MCS/3'Alu5' – HindIII – SphI – PstI – AccI – SalI – TEL – ClaI – EcoRV – SP6 promoter. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH252, Saccharomyces cerevisiae CGY2516 [ATCC 74013].
质粒图谱:
质粒序列:
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