pFL46S

质粒编号:
质粒名称: pFL46S
其他名称:
质粒大小（bp）: 6078
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: X70270
其他属性: pFL46S was constructed from pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site and the 2 micron 0.5 Kb FspI fragment containing its replication origin has been cloned at the ClaI site. The order of the major features in this plasmid is:EcoRI/MCS/HindIII – LEU2 – 2 micron ori – pMB1 ori – ampR. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 15.5. Restriction digests of the clone give the following sizes (kb): BamHI–6.1; BglII–3.3, 2.8; ClaI–3.7, 1.9, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 397135 Hosts: E.coli HB101, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pUC19, yeast 2-micron plasmid.
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