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- 库存：
1
- 英文名：
Pax-6 Polyclonal Antibody, Purified
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- Pax-6 Polyclonal Antibody, Purified保存于运输说明：
- Store at -20°C.
- 详细信息：
-
Description Polyclonal Antibody against Pax-6 Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Purified Antibody (Stored in PBS + 0.03% Thimerosal) Host Rabbit Species Reactivity Extensive [Ab] Pax-6 Polyclonal Antibody, Purified 2 mg/mL Specificity This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein. The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a Protein A column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes. Uses This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunofluorescence (IF) and staining of tissue sections. Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:200-500*
- IHC: 1:300
- IF: 1:300
Notes *Predicted MW = 47-50 kDa Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Pax-6 Polyclonal Antibody, Purified Marquardt T, Ashery-Padan R, Andrejewski N, Scardigli R, Guillemot F, Gruss P. Pax6 is required for the multipotent state of retinal progenitor cells. Cell 105(1):43-55, 2001.
Yamamoto Y, Jeffery WR. Central role for the lens in cave fish eye degeneration.Science 289(5479):631-633, 2000.
Ashery-Padan R, Marquardt T, Zhou X, Gruss P. Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye. Genes Dev 14(21):2701-2711, 2000.
Osumi N, Hirota A, Ohuchi H, Nakafuku H, Iimura T, Kuratani S, Fujiwara M, Noji S, Eto K. Pax-6 is involved in the specification of hindbrain motor neuron subtype.Development 124:2961-2972, 1997.
Koroma BM, Yang JM, Sundin OH. The Pax-6 homeobox gene is expressed throughout the corneal and
conjunctival epithelia. Invest. Ophthalmol Vis Sci 38:108-120, 1997.
Davis JA, Reed RR. Role of Olf-1 and Pax-6 transcription factors in neurodevelopment. J Neurosci 16:5082-5094, 1996. [IHC]Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Pax-6 Polyclonal Antibody, Purified
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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