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- 详细信息
- 技术资料
- 库存：
1
- 英文名：
PI(3)P Mass ELISA Kit
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- PI(3)P Mass ELISA Kit详细信息：
-
Description: The PI(3)P Mass ELISA measures the amount of PI(3)P extracted from cells by means of a competitive ELISA, (96 well format).
The assay plate method developed by Echelon Biosciences, Inc. allows the user to measure the amount of PI(3)P extracted from cells by means of standard competitive ELISA format, eliminating the need for radioactivity and thin layer chromatography.
The PI(3)P Mass ELISA Kit allows for the determination of PI3-K activity by quantifying the amount of PI(3)P found in cells. After following a non-radioactive lipid extraction protocol, the PI(3)P samples obtained from millions of cells are detected on a competitive ELISA. The cellular PI(3)P samples are mixed and incubated with a PI(3)P Detector protein, then added to a PI(3)P-coated microplate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the PI(3)P protein binding to the plate. The assay is sensitive to 1.5 pmol PI(3)P. The colorimetric signal is inversely proportional to the amount of PI(3)P obtained.PI(3)P Mass ELISA KitFormats
- 96-well Competitive ELISA
Storage
- 2-8°C, and -20°C
Reference
- and
- PI(3)P Mass ELISA Kit
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