- 询价
- R&D
- ZK-0002213
- 美国/中国
- 2025年11月20日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 靶点：
来电咨询
- 克隆性：
多克隆
- 标记物：
见 说 明 书
- 宿主：
Goat，Rabbit，Mouse
- 适应物种：
人，大鼠，小鼠，兔
- 形态：
液态/粉状
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 保存条件：
-20℃保存
- HMB-45 Monoclonal Antibody, Purified (SIGNET)详细信息：
-
Description Purified Monoclonal Antibody against Melanosome
(Formerly Signet Catalog Nos: 116-01, 116-16 L1 Predilute, 116-26 L2 Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Clone HMB-45 Form Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse Species Reactivity Human IsoType HMB-45 Monoclonal Antibody, Purified (SIGNET)IgG1 Specificity The HMB-45 antibody reacts with an intracytoplasmic antigen expressed by a majority of melanomas (melanotic and amelanotic) and other tumors expressing melanocytic differentiation. In general, epithelioid melanoma cells display more uniform staining than more sarcomatous spindle-shaped melanoma cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are negative. In normal cells, the antibody reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes (variable). Intradermal nevi, non-melanocytic cells and normal adult melanocytes are negative.
This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.
Concentrated Format:
SIG-3116-1000 [1 mL]
Prediluted Formats:
SIG-3116-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems such as Signet’s PAP (SIG-32140).
SIG-3116-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).Uses HMB-45 Monoclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:20 (concentrated format) with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250)
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20 minutes at room temperature
Notes Positive tissue (human): Melanoma - IHC: ≥1:20 (concentrated format) with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250)
- HMB-45 Monoclonal Antibody, Purified (SIGNET)
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HMB-45 Monoclonal Antibody, Purified (SIGNET)
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