- ￥3200
- Bioss
- IHC0363
- 2025年10月15日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 规格：
50T
| 产品编号 | IHC0363 |
| 英文名称 | CD3E Ready-To-Use IHC Kit |
| 中文名称 | CD3E即用型免疫组化试剂盒 |
| 英文别名 | 4930549J05Rik, A430104F18Rik, AI504783, AW552088, CD247, CD3, CD3-DELTA, Cd3-eta, CD3-GAMMA, CD3-ZETA, CD3D, CD3E, CD3epsilon, CD3G, CD3H, CD3Q, CD3Z, Cd3zeta, Ctg-3, Ctg3, IMD17, IMD18, IMD19, IMD25, T3D, T3E, T3G, T3Z, TCRE, Tcrk, TCRZ, CD3 epsilon, CD3-epsilon |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 亚基 | The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with CD6 (PubMed:15294938). Interacts (via Proline-rich sequence) with NCK1; the interaction is ligand dependent but independent of tyrosine kinase activation (PubMed:12110186, PubMed:15972658, PubMed:38614099). Interacts with NUMB; this interaction is important for TCR-CD3 internalization and subsequent degradation (PubMed:26507128). |
| 亚细胞定位 | #Cell membrane #Membrane |
| 翻译后修饰 | Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. |
| 功能 | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099). |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 背景资料 | The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency. |
| 应用 | 推荐稀释比例 |
Immunohistochemical analysis of paraffin embedded mouse spleen tissue slide using IHC0363 (CD3E Kit).
Immunohistochemical analysis of paraffin embedded rat spleen tissue slide using IHC0363 (CD3E Kit).
Immunohistochemical analysis of paraffin embedded human spleen tissue slide using IHC0363 (CD3E Kit).
Immunohistochemical analysis of paraffin embedded human small intestine tissue slide using IHC0363 (CD3E Kit).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
New double-positivity tool for multi-color IHC IF
by and reflected in local microenvironments, as well as how diseased cells respond to therapies. These kinds of questions can be answered in a limited way by methods that disaggregate or homogenize tissue, or that utilize conventional single-stain IHC
Imaging Techniques in Signal Transduction IHC
values within the dynamic range of the detector, frame averaging to eliminate noise (typically in fluorescence imaging), use of the highest bit depth when a choice is available, flatfield correction, and archiving of the image in a nonlossy format
的染色强度。获得合适的染色强度后，将载玻片置于蒸馏水 (dH2O) 中以阻止进一步显色。抗体：Phospho–Stat3(Tyr705)(D3A7) XP® Rabbit mAb #9145样本：石蜡包埋的人类乳腺肿瘤显色检测：利用 SignalStain® DAB Substrate Kit #8059（左），竞争者提供的 DAB（中和右）CST 的 IHC/IF 明星产品推荐：生化试剂：Catalog NumberProduct NameProduct
技术资料暂无技术资料 索取技术资料
CD3E Ready-To-Use IHC Kit(IHC0363)-50T
￥3200
