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文献和实验.This is an easy assay format for those with access to multiple channel pipettors and microtiter plate spectrophotometers. Materials Bradford Reagent - Bio-Rad sells a ready-to-use reagent (cat#500-0006)that can be stored at 4℃
Considerations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay.It is fairly accurate and samples that are out of range can be retested within minutes.The Bradford is recommended for general use
in room 4.278 General Overview of Procedure: Dilute 5x Bradford Reagent (BioRad) 4:1 with water and run through a gravity filter. Get a 96-well ELISA plate (Limbro microtitration plate). Turn on machine to allow bulb to warm
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