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1mg/1mL x 10mM (in DMSO)
| 规格: | 1mg | 产品价格: | ¥443.0 |
|---|---|---|---|
| 规格: | 1mL x 10mM (in DMSO) | 产品价格: | ¥1230.0 |
| 产品编号 | D55175 |
| 英文名称 | GSK872 HCl(1346546-69-7 free base) |
| 英文别名 | GSK2399872A |
| 理论分子量 | 419.94 |
| 保存条件 | Powder: -20℃ for 3 years | In solvent: -80℃ for 1 year |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GSK872 HCl is an effective and specific RIP3 kinase inhibitor. It binds RIP3 kinase domain with high affinity (IC50: 1.8 nM) and inhibits kinase activity (IC50: 1.3 nM). |
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文献和实验the gel: dissolve 0.8g agarose in 100mL of 10mM NaPO4 pH 7.0 (5mL of 0.2M NaP04 pH 7.0 + 95mL "DEPC" H2 O). Make sure gel box and comb are RNase-free by treatment with 3% H2 O2 or commericial RNase removal product (e.g. RNase Zap from Ambion).
Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
‐3′‐oxime (BIO, a GSK‐3 inhibitor; Sigma, cat. no. B1686) in DMSO (store up to 1 year at −80°C) 10 mM inhibitor of Wnt response‐1 (IWR, an Axin inhibitor; Sigma, cat. no. I0161) in DMSO
E-Z 96 X-press Plasmid Vacuum Protocol
collection plate as a support to give the collection plate a proper position. 15. Add 100-150 μl Elution Buffer (10mM Tris-HCl, pH 8.5) or sterile water to each well of the E-Z 96® X-press Plate, let stand for 2 minutes. Apply maximum vacuum for 5-10
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