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100ul
| 产品编号 | bsm-33264M-HRP |
| 英文名称 | RPS6KB1 Mouse mAb, HRP conjugated |
| 中文名称 | HRP标记的核糖体S6蛋白激酶单克隆抗体 |
| 英文别名 | RPS6KB1; p70(S6K)-alpha; ribosomal protein S6 kinase; p70 S6 Kinase; P70 beta1; KS6B1_HUMAN; Ribosomal protein S6 kinase beta-1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human RPS6KB1 Protein |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Cycle Inhibitors > Rb Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > DNA Damage Response > DNA Damage Recognition Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Ribosome Metabolism > Types of disease > Obesity Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > S6K Family |
| 亚基 | Interacts with PPP1R9A/neurabin-1. Interacts with RPTOR. Interacts with IRS1. Interacts with EIF3B and EIF3C. Interacts with POLDIP3 and TRAF4. |
| 亚细胞定位 | Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. |
| 组织特异性 | Widely expressed. |
| 翻译后修饰 | Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. |
| 相似性 | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. _x000D_ Contains 1 AGC-kinase C-terminal domain. _x000D_ Contains 1 protein kinase domain. |
| 功能 | Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
for 6 months without reducing binding activity. 2) This buffer can not be used for diluting HRP conjugated antibodies since sodium azide is an inhibitor of HRP. HRP-conjugated Primary Antibody Dilution Buffer: 1%BSA
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