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产品信息以Bioss网站为准
- 规格:
100ul
| 产品编号 | bsm-33004M-HRP |
| 英文名称 | His tag Mouse mAb, HRP conjugated |
| 中文名称 | HRP标记的聚组氨酸抗体标签单克隆抗体 |
| 英文别名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
| 产品应用 | WB=1:5000-100000 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated HHHHHH peptide |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Antibodies > 6x his tag® Tags & Cell Markers > Epitope Tags > His Tag |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:5000-100000} |

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文献和实验. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
) HRP‐conjugated anti‐flag antibody Tyramide signal amplification (TSA) kit (Life Technologies, cat. no. T30953) containing
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