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100ul
| 产品编号 | bs-0639R-AP |
| 英文名称 | GFP Rabbit pAb, AP conjugated |
| 中文名称 | AP标记的绿色荧光蛋白抗体 |
| 英文别名 | Green Fluorecent protein. |
| 中文别名 | 绿色荧光蛋白 GFP; 绿色荧光蛋白 (GFP); |
| 产品应用 | WB=1:20000-50000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from belt jellyfish GFP |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > GFP |
| 亚基 | Monomer. |
| 组织特异性 | Photocytes. |
| 翻译后修饰 | Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. |
| 相似性 | Belongs to the GFP family. |
| 功能 | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:20000-50000} |
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文献和实验Immunity:北大蒋争凡课题组发现 STING 后高尔基体囊泡转运的重要性及机制
: https://doi.org/10.1016/j.immuni.2023.02.004 参考文献: 1 Fang, R. et al. Recent advances in the activation and regulation of the cGAS-STING pathway. Advances in Immunology 156, 55-102, doi:10.1016 /bs.ai.2022.09.003 (2022). 2 Posor, Y. et al.
辨显微镜下,如果两种荧光出现在同一位置,那么就证明它们空间上较为接近,很有可能产生了相互作用。然而,就如上一句话里所说的,只是很有可能,并不能作为直接证据证明它们真的相互作用了。这个技术一般都是没办法时候的办法,就不举例子啦。【三】荧光双分子互补, bi-molecular fluorescence complementation(BiFC), 人们把绿色荧光蛋白 GFP 分子分割成两段,分别与接受测试的两个蛋白融合表达。如果两个蛋白相互作用,那么在细胞中 GFP 的两个片段就可以在空间上相互接近
给大家带来两篇CNS文章,为开发兼具安全性和有效性的CAR-T疗法提供思路。 c-Jun 提高CAR-T的有效性 2019年12月4号,斯坦福大学医学院Crystal L. Mackall教授课题组在《Nature》杂志发表的文章,揭示了CAR-T细胞中c-Jun过表达诱导T细胞耗竭抵抗[1]。研究人员构建了T细胞耗竭模型,通过ATAC-Seq技术,精确定位调节过表达或表达不足的基因组区域,找到了c-Jun基因。研究人员最终发现过表达经典AP-1因子c-Jun的CAR-T细胞具有增强扩增
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